Abstract:
A primer pair for certificating the species of Chinese cabbage, a method for certificating the species of Chinese cabbage by using the primer pair, and a kit containing the primer pair are provided to allow the species of the transformed Chinese cabbage to be certificated by PCR. A primer pair for certificating the species of Chinese cabbage is selected from the group consisting of a primer pair represented by the sequences 1 and 2; a primer pair represented by the sequences 1 and 3; and a primer pair represented by the sequences 1 and 4. A method for certificating the species of Chinese cabbage comprises the steps of extracting genome DNA from Chinese cabbage; carrying out PCR by using the primer pair with a template of the extracted genome DNA; and separating the PCR product by size.
Abstract:
The present invention relates to transgenic rice producing capsanthin, which is obtained by breeding rice transformed with a plant transformation expression vector containing a capsanthin-capsorubin synthase gene and zeaxanthin-producing rice transformed with a plant transformation expression vector containing a β-carotene hydroxylase gene. The transgenic rice according to the present invention has a high content of zeaxanthin and exhibits red color while producing capsanthin, a red pigment of red pepper, and thus can be used as functional special crops.
Abstract:
본 발명은 지아산틴 생성능력이 우수한 재조합 벡터 및 상기 재조합 벡터로 형질전환된 형질전환 세포 및 식물에 관한 것이다. 본 발명에 따른 재조합 벡터 및 형질전환 세포를 이용하면 카로티노이드 물질 중 특히 지아산틴 함량이 높은 형질전환 식물을 제조할 수 있는 바, 시력개선 효과 등을 기대할 수 있는 기능성 특수 작물을 제공할 수 있다. 본 발명에 따른 형질전환 식물은 지아산틴 함량이 증가되어 황금색을 나타내므로, 이를 이용하여 항산화성 천연색소 생산을 통한 새로운 컬러작물 개발 기반을 확립하고, 새로운 기능성 컬러 GM 작물 소재 개발을 위한 고유 유전자 및 운반체 IP를 확보할 수 있다. 또한 자연계에 없는 새로운 특수 작물 개발로 장기적으로 기능성 작물의 다양성 확대 및 소비 촉진 기대되며 지아산틴 생산작물의 사료화 등을 통한 농업의 신규한 산업 소재 개발에 도움이 될 수 있다.
Abstract:
PURPOSE: A novel polynucleotide which generates keto-carotenoid such as astaxanthin is provided to produce a transgenic plant which generates two kinds of keto-carotenoid(astaxanthin and canthaxanthin). CONSTITUTION: A novel polynucleotide which generates keto-carotenoid such as astaxanthin contains a nucleic acid of sequence number 1 or 2. A recombinant vector containing the polynucleotide of sequence number 1 or 2 additionally contains a polynucleotide with a nucleic acid of sequence number 3 or 4. The recombinant vector is pGlb:BAC, pGlb:stBAC, pGlb:BAC-PAC, or pGlb:stBAC-PAC vector. A cell which is transformed with the recombinant vector is an Agrobacterium sp. microorganism or a plant cell. A transgenic plant containing the recombinant vector is Oryza sativa, corn, or barley. [Reference numerals] (AA) Normal rice; (BB) Beta-carotene rice; (CC) Astaxanthin rice; (DD) Recombinant gene name; (EE) Patent no. 10-0905219; (FF) Reference
Abstract:
본 발명은 무 자가불화합성 유전자의 유전자형 판별 프라이머 세트 및 무 자가불화합성 유전자의 유전자형 판별방법에 관한 것으로, 상세하게는 무의 자가불화합성 유전자인 SLG 및 SRK의 유전자형을 판별함으로써 동일한 유전자형의 자가불화합성 유전자를 가진 무 계통간의 교배로 인한 수분 및 수정 실패를 미연에 방지할 수 있는 무 자가불화합성 유전자형 판별방법에 관한 것이다. 무, 자가불화합성, 프라이머, 육종
Abstract:
PURPOSE: A promoter which petal-specifically induces expression of a foreign gene in a plant is provided to be used for transformation of various crops in a genetic engineering method. CONSTITUTION: A petal-specific promoter is a promoter of Nicotiana tabacum ANS(anthocyanidin synthase) gene of sequence number 1 or 2. The promoter induces the expression of petal-specific gene. The promoter has a base sequence of sequence number 3. A primer set for amplifying the promoter contains a primer of sequence number 4 and a primer of sequence number 5. A pBGWFS7-PNtANS expression vector contains the promoter. A method for petal-specifically expressing a foreign gene comprises: a step of preparing an expression vector containing the promoter and the foreign gene; and a step of transducing the expression vector to a plant.