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公开(公告)号：KR1020040047334A
公开(公告)日：2004-06-05
申请号：KR1020020075495
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Specific primers for detecting watermelon mosaic virus 2 are provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, detecting all kinds of watermelon mosaic virus 2, and improving the detection accuracy by removing nonspecific reaction with other related viruses. CONSTITUTION: The specific primers for detecting watermelon mosaic virus 2 comprise a nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2 which species specifically binds with watermelon mosaic virus 2, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof; and another nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 7 which is combined with the selected nucleic acid and species specifically binds with watermelon mosaic virus 2, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.
Abstract translation: 目的：提供检测西瓜花叶病毒2的特异性引物，提高检测限和灵敏度，比使用抗血清高出约1000倍，检测各种西瓜花叶病毒2，通过去除与其他相关病毒的非特异性反应提高检测准确度 。 构成：用于检测西瓜花叶病毒2的特异性引物包含选自SEQ ID NO：1或SEQ ID NO：2所示核苷酸序列的核酸片段，该核苷酸序列与西瓜花叶病毒2特异性结合，其互补核苷酸序列， 及其突变核苷酸序列; 和选自SEQ ID NO：7所示的核苷酸序列的另一个核酸序列，其与所选择的核酸和物种组合，与西瓜花叶病毒2，其互补核苷酸序列及其突变核苷酸序列特异性结合，其中突变 选自碱基的缺失，碱的加入，碱的取代或其组合。
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公开(公告)号：KR101650551B1
公开(公告)日：2016-08-24
申请号：KR1020110117664
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 1 내지 12의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 AMV (Alfalfa mosaic virus) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 AMV 진단또는검출용유전자칩, AMV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 AMV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 AMV 또는검출용키트에관한것이다.
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公开(公告)号：KR101650550B1
公开(公告)日：2016-08-24
申请号：KR1020110117658
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 2122 내지 2125 및서열번호 2131의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 PSTVd (Potato spindle tuber viroid) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 PSTVd 진단또는검출용유전자칩, PSTVd에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 PSTVd를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 PSTVd 또는검출용키트에관한것이다.
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公开(公告)号：KR101650547B1
公开(公告)日：2016-08-24
申请号：KR1020110117624
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 894 내지 909의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 SqMV (Squash mosaic virus) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 SqMV 진단또는검출용유전자칩, SqMV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 SqMV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 SqMV 진단또는검출용키트에관한것이다.
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公开(公告)号：KR101407237B1
公开(公告)日：2014-07-01
申请号：KR1020110117611
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본 발명은 카모바이러스 속 (Genus Carmovirus) 바이러스에 특이적인 41개의 올리고뉴클레오티드 및 이의 상보적 올리고뉴클레오티드로 구성되는 군으로부터 선택되는 하나 이상의 올리고뉴클레오티드를 포함하는 카모바이러스 속 바이러스 진단 또는 검출용 올리고뉴클레오티드 프로브 세트, 상기 올리고뉴클레오티드 프로브 세트를 포함하는 카모바이러스 속 바이러스 진단 또는 검출용 유전자 칩, 카모바이러스 속 바이러스에 감염된 것으로 추정되는 시료로부터 RNA를 분리하는 단계, 상기 분리된 RNA를 주형으로 하여 cDNA를 합성하는 단계, 상기 합성된 cDNA를 상기 올리고뉴클레오티드 프로브 세트와 접촉시켜 상기 cDNA와 프로브 세트를 혼성화시키는 단계 및 상기 프로브와 cDNA 사이의 혼성화 정도를 검출하는 단계를 포함하는 카모바이러스 속 바이러스를 진단 또는 검출하는 방법 및 상기 올리고뉴클레오티드 프로브 세트 및 혼성화 용액을 포함하는 카모바이러스 속 바이러스는 검출용 키트에 관한 것이다.
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Patent Agency Ranking
公开(公告)号：KR1020140030344A
公开(公告)日：2014-03-12
申请号：KR1020110117668
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: The present invention relates to an Arabis mosaic virus (ArMV) oligonucleotide probe set which includes one or more oligonucleotides selected from a group including oligonucleotides represented by sequence numbers of 1481 to 1485, 1659 to 1664, 1720 to 1721, and 1801 to 1808, and complementary oligonucleotide thereof, to a DNA chip for diagnosing or detecting the ArMV which includes the oligonucleotide probe set, to a method for diagnosing or detecting the ArMV which comprises the steps of: isolating RNA from samples which are assumed to be infected by the ArMV; synthesizing cDNA using the isolated RNA as a template; hybridizing the cDNA with the probe set by touching the synthesized cDNA with the oligonucleotide probe set; and detecting the degree of hybridization between the probe and cDNA, and to a kit for detecting the ArMV which includes the oligonucleotide probe set and a hybridization solution. [Reference numerals] (AA) Kidney bean
Abstract translation: 本发明涉及一种含有一种或多种寡核苷酸探针组的阿拉维氏花叶病毒（ArMV）寡核苷酸探针组，所述寡核苷酸探针组包含一个或多个选自由序列号1481至1485,1659至1664,1720至1721和1801至1808所代表的寡核苷酸的寡核苷酸，以及 涉及用于诊断或检测包含寡核苷酸探针组的ArMV的DNA芯片，涉及用于诊断或检测ArMV的方法，其包括以下步骤：从假定被ArMV感染的样品中分离RNA; 使用分离的RNA作为模板合成cDNA; 通过用寡核苷酸探针组接触合成的cDNA将cDNA与探针组杂交; 并检测探针和cDNA之间的杂交程度，以及检测包含寡核苷酸探针组和杂交溶液的ArMV的试剂盒。 （附图标记）（AA）芸豆
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公开(公告)号：KR1020130054501A
公开(公告)日：2013-05-27
申请号：KR1020110117791
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting TSWV(tomato spotted wilt virus) is provided to minimize damage due to viral diseases and to enhance production. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting TSWV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 3449-3476 and 3513-3520, and complementary oligonucleotides thereof. A method for diagnosing or detecting TSWV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using the RNA as a template; a step of hybridizing the cDNA with the probe set; and a step of detecting hybridization. [Reference numerals] (AA) Capsicum spp. pochungchun; (BB) Host: Capsicum spp. pochungchun
Abstract translation: 目的：提供用于诊断或检测TSWV（番茄斑点枯萎病毒）的探针组，以尽量减少由于病毒性疾病引起的损伤并提高产量。 构成：用于诊断或检测TSWV的寡核苷酸探针组含有选自序列号3449-3476和3513-3520的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测TSWV的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 和检测杂交的步骤。 （AA）辣椒属（AA） pochungchun; （BB）主持人：辣椒属 pochungchun
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公开(公告)号：KR1020130054499A
公开(公告)日：2013-05-27
申请号：KR1020110117789
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting LRSV(lychnis ring-spot virus) is provided to minimize damage due to viral diseases in crops and to increase product yield. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting LRSV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1307-1311 and complementary oligonucleotides thereof. A method for diagnosing or detecting LRSV comprises: a step of isolating RNA from a sample and synthesizing cDNA; a step of hybridizing cDNA with the probe set; and a step of detecting hybridization.
Abstract translation: 目的：提供用于诊断或检测LRSV（lychnis环斑病毒）的探针，以尽量减少作物病毒性疾病引起的损伤，并提高产品产量。 构成：用于诊断或检测LRSV的寡核苷酸探针组含有一种或多种选自序列号1307-1311的寡核苷酸和其互补寡核苷酸的寡核苷酸。 用于诊断或检测LRSV的方法包括：从样品中分离RNA并合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 和检测杂交的步骤。
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公开(公告)号：KR1020130054485A
公开(公告)日：2013-05-27
申请号：KR1020110117615
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting TBSV(Tomato bushy stunt virus) is provided to minimize damage to viral diseases in crops and to enhance the yield of crops. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting TBSV contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence number 3432-3438 and complementary oligonucleotides thereof. A method for diagnosing or detecting TBSV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting hybridization. [Reference numerals] (AA) Koko tomato; (BB) Host: Tenten tomato
Abstract translation: 目的：提供用于诊断或检测TBSV（番茄丛生特技病毒）的探针，以尽量减少作物病毒性疾病的损害，并提高作物的产量。 构成：用于诊断或检测TBSV的寡核苷酸探针组含有一种或多种选自序列号3432-3438的寡核苷酸及其互补寡核苷酸的寡核苷酸。 一种用于诊断或检测TBSV的方法包括：从样品中分离RNA的步骤; 合成cDNA的步骤; cDNA和探针组杂交的步骤; 和检测杂交的步骤。 （AA）科科番茄; （BB）主持人：Tenten番茄
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公开(公告)号：KR1020120119457A
公开(公告)日：2012-10-31
申请号：KR1020110037395
申请日：2011-04-21
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6851 , C12Q1/706 , C12Q2600/16
Abstract: PURPOSE: A specific primer for soybean virus multiplex diagnosis is provided to reduce diagnosing cost and efforts by propagation of diagnosing method for various viruses caused in the soybean. CONSTITUTION: A specific primer for soybean virus multiplex diagnosis comprises A pair of primers represented by the sequence number 1(SEQ ID NO:1) and the sequence number 2, a pair of primers represented by the sequence number 3, 4, 5, and 6, a pair of primers represented by the sequence number 7 and 8, and a pair of primers represented by the sequence number 9 and 10. The specific primer for soybean virus multiplex diagnosis additionally includes a pair of primers represented by 11 and 12. The specific primer can concurrently diagnose AMV, SYMMV and USV3.
Abstract translation: 目的：提供大豆病毒多重诊断的具体引物，以减少大豆中引起的各种病毒诊断方法的诊断成本和努力。 构成：用于大豆病毒多重诊断的具体引物包括由序列号1（SEQ ID NO：1）和序列号2表示的一对引物，由序列号3,4,5和 6，由序列号7和8表示的一对引物和由序列号9和10表示的一对引物。大豆病毒多重诊断的特异性引物另外包括由11和12表示的一对引物。 特异性引物可同时诊断AMV，SYMMV和USV3。
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