Abstract:
PURPOSE: A gene analysis apparatus is provided to increase intensity of a fluorescence light by bioreaction. CONSTITUTION: A gene analysis apparatus comprises: an illuminating optical system for irradiating a sample solution with an excitation light; a microfluidic device(110) comprising one or more microchambers(115) with a reflection pattern; a detection optical system comprising a photodetector for detecting a fluorescence light generated by bioreaction in the microchambers. The microfluidic device is formed of a silicon substrate material or a polymer material.
Abstract:
PURPOSE: A method for enhancing PCR sensitivity and speed and a composition for the same are provided to quickly detect target microorganisms, cells or genes in a sample. CONSTITUTION: A method for enhancing PCR efficiency and speed comprises: a step of preparing a biological sample and PCR mixture and adding alkali to one or both; and a step of mixing the biological sample and PCR mixture and performing PCR. The biological sample includes cells, body fluid, or nucleic acids. The alkali is sodium hydroxide or potassium hydroxide. A composition for efficient PCR contains the PCR mixture and alkali.
Abstract:
본 발명의 예시적 구체예는, 제1 pH에서 양전하를 띠고 제2 pH에서 음전하를 띠는 화합물 및 제1 pH에서 상기 화합물에 핵산을 결합시키고, 제2 pH에서 핵산을 상기 화합물로부터 분리하는 단계를 포함하는 핵산을 분리하는 방법을 제공한다. 핵산, 분리
Abstract:
PURPOSE: A dielectric heating method using microwaves and anions is provided to reduce heating time of objects by increasing a heating rate through the addition of negative ions with high charge density promoting polarization of molecule. CONSTITUTION: A dielectric heating method using microwaves and anions comprises the steps of: adding negative ions with high charge density, which interacts with a medium molecule strongly than a hydrogen bond between medium molecules of dielectric, to a dielectric for dielectric heating; and irradiating microwaves to the dielectric to heat the dielectric.
Abstract:
본 발명은 비평면 형상의 고체 지지체와, 미생물을 포함하는 혈액 배양물을 pH 3.0 내지 6.0의 범위에서 접촉시켜 상기 미생물을 상기 고체 지지체에 부착시키는 단계, 상기 고체 지지체에 부착되지 않은 물질을 세척하여 제거하는 단계, 및 상기 고체 지지체에 부착된 상기 미생물에 대하여 PCR을 수행하여 핵산을 증폭하는 단계를 포함하는, 미생물로부터 핵산을 증폭하는 방법으로서, 상기 접촉단계, 세척 단계, 및 증폭 단계는 동일한 용기 내에서 수행되는 것을 특징으로 하는 방법을 제공한다. 비평면 형상, 고체 지지체, 핵산, 증폭
Abstract:
A method for amplifying a nucleic acid from a microorganism is provided to perform a separation of the microorganism, a cell lysis and a nucleic acid amplification in one container, thereby capable of amplifying the nucleic acid conveniently and rapidly with high sensitivity. A method for amplifying a nucleic acid from a microorganism such as bacteria, fungi or virus comprises the steps of: (a) contacting a non-planar solid substrate with a blood culture material including the microorganism under pH of 3.0-6.0 to attach the microorganism to the solid substrate; (b) washing materials not attached to the solid substrate to remove the materials; and (c) subjecting the microorganism attached to the solid substrate to PCR, wherein all the steps are performed in a same container, the blood culture material includes polyanethole sulfonate, the sample is diluted by a phosphate buffer or an acetate buffer, and the solid substrate is selected from the group consisting of a solid substrate having a pillar structure where a plurality of pillar is formed, a bead-shaped solid substrate and a sieve-structured solid substrate, has a water contact angle of 70-95° and includes at least one amine-based functional group on the surface thereof.
Abstract:
A method for amplifying a nucleic acid from a microorganism is provided to amplify conveniently, rapidly and highly sensitively a nucleic acid by performing separation of the microorganism, cell lysis and amplification of a nucleic acid in one container and use a large amount of an initial sample due to contact the microorganism with high fluid speed with a solid substrate. A method for amplifying a nucleic acid from a microorganism such as bacteria, fungi or virus comprises the steps of: (a) contacting a non-planar solid substrate with a biological sample including the microorganism under pH of 3.0-6.0 to attach the microorganism to the solid substrate; (b) washing materials not attached to the solid substrate to remove the materials; and (c) subjecting the microorganism attached to the solid substrate to PCR, wherein all the steps are performed in a same container and the solid substrate is selected from the group consisting of a solid substrate having a pillar structure where a plurality of pillar is formed, a bead-shaped solid substrate and a sieve-structured solid substrate, has a water contact angle of 70-95° and includes at least one amine-based functional group on the surface thereof.
Abstract:
본 발명은 삼차원 지지체에 관한 것으로, 보다 상세하게는 반도체 제조 공정에서 사용되는 포토레지스트 노광공정을 사용하여 용이하게 제작할 수 있는 삼차원 구조를 가지는 삼차원 지지체에 관한 것이다. 본 발명에 따른 삼차원 지지체는 입체적인 구조를 가지는 삼차원 지지체로서, 그 재질은 포토레지스트이고 유체가 상, 하부로 통과하는 구조로 된 삼차원 구조가 일정한 간격으로 반복하여 배치된 것을 특징으로 한다. 삼차원, 지지체, 포토레지스트, 노광공정
Abstract:
본 발명은 결착 수지, 착색제, 대전제어제 및 이형제를 포함하는 토너 입자; 및 상기 토너 입자의 표면에 첨가되는 외첨제를 포함하는 전자사진용 현상제에 있어서, 상기 대전제어제는 경금속계 착체 화합물이고, 상기 이형제는 고체 지방산 에스테르계 물질이고, 이형제의 함량이 토너 입자를 기준으로 하여 1.5 내지 4.0 중량%인 전자사진용 현상제를 제공한다. 또한 상기 전자사진 현상제를 채용하는 전자사진 화상형성용 장치를 제공한다. 본 발명에 따른 현상제는 토너 제조시에 대전제어제로서 경금속 착제를 사용하고 이형제로서 고체 지방산 에스테르계 물질 1.5 내지 4.0중량%를 사용함으로써 장수명에 걸쳐 안정적인 대전량을 분포하게 할 수 있고, 우수한 토너 유동성 및 현상성을 유지할 수 있다. 이에 따라 오염, 비산 발생 억제, 및 현상 효율에 의하여 토너 내구성 향상을 도모할 수 있어서 고화질의 화상을 얻을 수 있다.
Abstract:
A microfluidic device is provided to allow a sample having the designed volume to be flown in a chamber by preventing the expansion of a chamber division membrane, and promote the electric current flowing by distributing an anode electrode to a trapezoid shape, thereby efficiently performing the pH regulation. And a method for regulating the pH of a fluid in the microfluidic device through electrolysis using the same device is provided. The microfluidic device comprises an ion exchange membrane(105), an anode chamber(109), one side of which is composed of one side of the ion exchange membrane, including a trapezoid-shaped anode electrode(103) and an anode electrode supporting portion(115), and a cathode chamber, one side of which is composed of the other side of the ion exchange membrane, including a cathode electrode(101), wherein the anode electrode supporting portion includes a trapezoid-shaped anode electrode on one side thereof, in which an opening portion(125) is formed along the trapezoid-shape, and supports the ion exchange membrane by the other side thereof contacted with the ion exchange membrane. Each of the anode and the cathode chambers further comprises a gas outlet. In the device, the electrode supporting portion comprises a printed circuit board, the cathode electrode is Pd and the anode electrode is selected from the group consisting of Cu, Pb, Ag, Cr, Ti, Ni, Zn, and Sn. The method for regulating the pH of a fluid comprises the steps of: (a) allowing a solution including ions having higher or lower standard oxidation potential than that of water to be flown in an anode chamber; (b) allowing a solution including ions having lower standard oxidation potential than that of water to be flown in a cathode chamber; and (c) applying electric current to the anode and cathode chambers through the anode and cathode electrodes to cause electrolysis at the anode and cathode chambers and regulate the pH of the solution flown in either the anode chamber or the cathode chamber.