살모넬라 혈청형의 분자적 동정을 위한 프라이머 및 이를 이용한 살모넬라 혈청형 동정 방법
    34.
    发明公开
    살모넬라 혈청형의 분자적 동정을 위한 프라이머 및 이를 이용한 살모넬라 혈청형 동정 방법 有权
    用于分子鉴定SALMONELLA SEROTYPE的引物和使用其鉴定SALMONELLA SEROTYPE的方法

    公开(公告)号:KR1020130076069A

    公开(公告)日:2013-07-08

    申请号:KR1020110144486

    申请日:2011-12-28

    CPC classification number: C12Q1/689 C12Q1/6869 Y02A50/451 C12R1/42

    Abstract: PURPOSE: A method of identifying salmonella serotype is provided to obtain more objective and correct result than that of existing method which uses an antiserum, apply to all Salmonella strains, conduct a simple process and reduce time and cost. CONSTITUTION: A method of identifying salmonella serotype comprises a step which amplifies the whole base sequence of the beta subunit (rpoB) gene of RNA polymerase. A method of identifying salmonella serotype comprises; (1) a step which amplifies the whole base sequence of the rpoB gene of the Salmonella microorganism; (2) a step which determines the whole base sequence of the amplified rpoB gene; (3) a step which determines serotype by comparing the base sequence obtained in the previous step (2) with the base sequence of the rpoB gene of ordinary strain.

    Abstract translation: 目的:提供一种识别沙门氏菌血清型的方法,以获得比使用抗血清的现有方法更客观和更正确的结果,适用于所有沙门氏菌菌株,进行简单的过程并缩短时间和成本。 构成:识别沙门氏菌血清型的方法包括扩增RNA聚合酶的β亚单位(rpoB)基因的整个碱基序列的步骤。 识别沙门氏菌血清型的方法包括: (1)扩增沙门氏菌微生物的rpoB基因的整个碱基序列的步骤; (2)确定扩增的rpoB基因的整个碱基序列的步骤; (3)通过将前一步骤(2)获得的碱基序列与普通菌株的rpoB基因的碱基序列进行比较来确定血清型的步骤。

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