公开(公告)号：US20230182127A1

公开(公告)日：2023-06-15

申请号：US18076667

申请日：2022-12-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal Sharma ,  Ronald Lebofsky ,  Man Cheng ,  Adam McCoy ,  Kalyan Handique ,  Eric Pomaranski

IPC: B01L3/00 ,  C12N13/00

CPC classification number: B01L3/502 ,  C12N13/00 ,  B01L2200/026 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/0893

Abstract: Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

公开(公告)号：US11578322B2

公开(公告)日：2023-02-14

申请号：US16867256

申请日：2020-05-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US11547995B2

公开(公告)日：2023-01-10

申请号：US17499529

申请日：2021-10-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686

Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

公开(公告)号：US11358147B2

公开(公告)日：2022-06-14

申请号：US17333337

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Javdani Sepehri ,  Kyle Gleason

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  C12M1/00 ,  C12Q1/6834 ,  C12Q1/6841 ,  G01N33/574 ,  G01N15/00

Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US11237096B2

公开(公告)日：2022-02-01

申请号：US17387716

申请日：2021-07-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20 ,  G06K9/00

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20210283608A1

公开(公告)日：2021-09-16

申请号：US17333142

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20210171939A1

公开(公告)日：2021-06-10

申请号：US17109704

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam McCoy ,  Kalyan Handique ,  Jonathan Bell ,  Vishal Sharma ,  Sida Wang

IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

Abstract: An embodiment of a composition for target material separation includes: a body and one or more molecules coupled to the body and structured for functionalization of the composition. In embodiments, each of the one or more molecules can include one or more of: a linker region; a polymerase chain reaction (PCR) segment or oligo binding region; one or more barcode region(s); a unique molecule identifier; a preparation-facilitating segment; an active segment; and a molecular scissor or cleavage region, wherein various regions can be coupled together in order to provide functionality to the composition. The invention(s) also cover manufacturing of the composition and various applications of use, in the context of target material capture (e.g., from single cells or other biological material).

公开(公告)号：US10794817B1

公开(公告)日：2020-10-06

申请号：US16924492

申请日：2020-07-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: B01L3/00 ,  G01N1/20 ,  G01N15/10 ,  C12M3/00 ,  G01N1/28 ,  G01N1/40 ,  G01N15/14 ,  B01L3/02 ,  C12M1/00 ,  G01N35/00 ,  G06K9/00 ,  G01N15/00

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US12290813B2

公开(公告)日：2025-05-06

申请号：US17330663

申请日：2021-05-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky ,  Josh Shinoff

IPC: C12Q1/00 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6806

Abstract: Methods and systems for sample target molecules are provided.

公开(公告)号：US20250050346A1

公开(公告)日：2025-02-13

申请号：US18927689

申请日：2024-10-25

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Kalyan Handique ,  Ronald Lebofsky ,  Emilie Celine Claudie Macke ,  Rebecca Dievart ,  Christophe Quiring ,  Sophie Pierre

IPC: B01L3/00

Abstract: A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.