公开(公告)号：US11578322B2

公开(公告)日：2023-02-14

申请号：US16867256

申请日：2020-05-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US20210283608A1

公开(公告)日：2021-09-16

申请号：US17333142

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US12152980B2

公开(公告)日：2024-11-26

申请号：US18633224

申请日：2024-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/02 ,  G01N15/01 ,  G01N15/10 ,  G01N15/149

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US12044614B2

公开(公告)日：2024-07-23

申请号：US17589507

申请日：2022-01-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  G01N15/00 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20230015223A1

公开(公告)日：2023-01-19

申请号：US17951719

申请日：2022-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US12066373B2

公开(公告)日：2024-08-20

申请号：US17951719

申请日：2022-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/02 ,  G01N15/01 ,  G01N15/10 ,  G01N15/149

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N15/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20230037836A1

公开(公告)日：2023-02-09

申请号：US17965495

申请日：2022-10-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20220155215A1

公开(公告)日：2022-05-19

申请号：US17589507

申请日：2022-01-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240255412A1

公开(公告)日：2024-08-01

申请号：US18633224

申请日：2024-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  William Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/149 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N15/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240253038A1

公开(公告)日：2024-08-01

申请号：US18233819

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/149 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC classification number: B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N15/149

Abstract: A system and method, wherein the method includes: (a) receiving a fluid comprising a process reagent for an assay and nucleic acid material from a sample into a fluid pathway coupled to an array of wells, wherein the array of wells is defined within a substrate and each well in the array of wells extends vertically into a planar surface of the substrate; (b) distributing the fluid along the fluid pathway and into wells of the array of wells; and (c) distributing a liquid immiscible with the fluid comprising the process reagent and nucleic acid material along the fluid pathway upon positive pressure generation by a pump of a flow control system in communication with an inlet to the fluid pathway, thereby displacing said fluid along the fluid pathway and into wells of the array of wells and preventing contents of each well from transferring to adjacent wells.