公开(公告)号：US20190267228A1

公开(公告)日：2019-08-29

申请号：US16348381

申请日：2017-11-02

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/42 ,  H01J49/06

Abstract: In one aspect, an ion trap is disclosed, which includes a curved linear ion trap having a plurality of electrodes arranged around a central curved axis so as to provide a volume for trapping ions, said plurality of electrodes comprising at least one inner electrode and at least one outer electrode radially separated from said inner electrode. The ion trap further includes a pair of inner and outer ion guide electrodes providing a volume therebetween for receiving ions ejected from said curved ion trap and guiding the ejected ions to one or more spatial locations along a focal line, said inner and outer ion guide electrodes being positioned external to said ion trapping volume and in proximity of said at least inner and outer electrodes of the curved ion trap, respectively, wherein a DC voltage is applied between said ion guide electrodes to provide an electric filed therebetween for guiding the ejected ions to said spatial locations.

公开(公告)号：US10032614B2

公开(公告)日：2018-07-24

申请号：US15316915

申请日：2015-06-04

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba ,  John Lawrence Campbell

IPC: G01N33/92 ,  H01J49/00 ,  H01J49/42

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds of sample molecules. The fragments may include radical fragments and non-radical fragments. The apparatus may also include an oxygen gas source configured to react with the radical fragments to produce oxygen-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules.

公开(公告)号：US09837256B2

公开(公告)日：2017-12-05

申请号：US15105130

申请日：2014-11-18

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba

IPC: H01J49/26 ,  H01J3/00 ,  H01J49/00 ,  H01J49/06 ,  H01J49/42

CPC classification number: H01J49/0095 ,  H01J49/0072 ,  H01J49/062 ,  H01J49/4225 ,  H01J49/4295

Abstract: A mass spectrometer ion reaction device, useful for performing ion-ion reactions (eg. ETD, PTR) is described. The device includes a plurality of non-linear rods, that form a pair of quadrupole rod sets. The device includes an axial passageway, that allows injections of ions of both polarities into the device, and a three dimensional trapping region. Anions and cations that are injected into the device are spatially separated into different trapping regions by a DC dipole electric field generated by a DC voltage source. The device also includes a plurality of lenses to confine, transmit or receive ions in/from the device.

公开(公告)号：US20170250064A1

公开(公告)日：2017-08-31

申请号：US15513271

申请日：2015-10-14

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/26 ,  B01D59/48

CPC classification number: H01J49/26 ,  B01D59/48 ,  H01J37/317 ,  H01J49/02 ,  H01J49/288 ,  H01J49/48

Abstract: A static magnetic field is applied in the same direction using a first magnet and a second magnet. The magnetic field is applied to a gap of uniform thickness between the first and second magnets. Voltages are applied to three or more electrodes located in the gap using a switchable voltage source. The voltages are applied to create a static electric field perpendicular to the static magnetic field in an input channel and at least one channel of two or more output channels. The channels are defined by gaps between the three or more electrodes. The simultaneous application of the static magnetic field and the static electric field causes ions from an ion beam of a mass spectrometer to move into and through the input channel and through at least one channel of the two or more output channels to another location in the mass spectrometer.

公开(公告)号：US20160217987A1

公开(公告)日：2016-07-28

申请号：US15089527

申请日：2016-04-02

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0036 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/04 ,  H01J49/067 ,  H01J49/4285

Abstract: Systems and methods for identifying precursor ions of product ions from combined product ion spectra are provided. N precursor ions are selected. N groups of the N precursor ions are created. The tandem mass spectrometer is instructed to perform multiplexed precursor ion selection on the continuous beam of ions, fragment each of the N−1 precursor ions, and measure the intensities of the product ions, producing N product ion spectra. A heat map is plotted, producing N heat maps. The N product ion spectra are combined into a combined product ion spectrum. A corresponding precursor ion of a peak is identified by finding a heat map of the N heat maps that does not have data for the mass of the peak and determining that a precursor ion of the N precursor ions that is not included in a group that produced the heat map is the corresponding precursor ion.

Abstract translation: 提供了用于从组合产物离子光谱中鉴定产物离子的前体离子的系统和方法。 选择N前体离子。 产生N组N前体离子。 指示串联质谱仪在连续的离子束上进行多重前体离子选择，分割每个N-1前体离子，并测量产物离子的强度，产生N个产物离子谱。 绘制热图，产生N个热图。 将N产物离子光谱合并成合成的产物离子光谱。 通过找到没有峰的质量的数据的N个热图的热图，并且确定未包括在产生的组中的N个前体离子的前体离子来识别峰的对应的前体离子 热图是相应的前体离子。

公开(公告)号：US11004669B2

公开(公告)日：2021-05-11

申请号：US16063104

申请日：2016-12-15

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba ,  Paul Baker ,  John Lawrence Campbell ,  Yves Le Blanc

IPC: H01J49/00 ,  G01N33/92

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds and/or the orientation of those double bonds within the sample molecules. The fragments may include radical fragments and non-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules as confirmation of the presence of those radical fragments.

公开(公告)号：US20210104299A1

公开(公告)日：2021-04-08

申请号：US17042108

申请日：2019-03-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba

IPC: G16B40/10 ,  G01N33/68 ,  G16C20/20 ,  H01J49/00

Abstract: A mass isolation device selects a precursor ion of a sample that has been digested using a protease. A first fragmentation device fragments the precursor ion using collision-induced dissociation (CID), and the resulting product ions are analyzed using a mass analyzer producing a CID spectrum. A list of theoretical candidate glycopeptide sequences is determined from CID spectrum. The mass isolation device again selects the precursor ion of the sample. A second fragmentation device fragments the precursor ion using electron-based dissociation (ExD), and the resulting product ions are analyzed using the mass analyzer producing a CID spectrum. For each sequence of the list, the sequence is computationally fragmented, producing theoretical fragments, mass-to-charge ratio (m/z) values are calculated for the theoretical fragments, and the sequence is scored using c and z fragment matching rules. The highest scoring sequence is identified as a peptide sequence of a glycopeptide of the sample.

公开(公告)号：US20210082678A1

公开(公告)日：2021-03-18

申请号：US17041324

申请日：2019-04-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi Baba ,  Pavel Ryumin ,  William M. Loyd

IPC: H01J49/40 ,  G01N33/68 ,  G16B40/10

Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. M An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.

公开(公告)号：US10256087B2

公开(公告)日：2019-04-09

申请号：US15329376

申请日：2015-07-24

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/40 ,  H01J49/42 ,  H01J49/06

Abstract: A multipole rod set of an ion guide is adapted to receive a radial RF trapping voltage and a radial dipole direct current DC voltage. A lens electrode of the ion guide is positioned at one end of the multipole rod set to extract ions from the multipole rod set and adapted to receive an axial trapping AC voltage and a DC voltage. A radial dipole DC voltage is applied to the multipole rod set and an axial trapping AC voltage is simultaneously applied to a lens electrode in order to extract a bandpass mass range of ions trapped in the multipole rod set. Alternatively, a radial RF trapping voltage amplitude is applied to the multipole rod set and an axial trapping AC voltage is simultaneously applied to the lens electrode in order to extract a bandpass mass range of ions trapped in the multipole rod set.

公开(公告)号：US10256083B2

公开(公告)日：2019-04-09

申请号：US15089527

申请日：2016-04-02

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/06 ,  H01J49/04 ,  H01J49/42

Abstract: Systems and methods for identifying precursor ions of product ions from combined product ion spectra are provided. N precursor ions are selected. N groups of the N precursor ions are created. The tandem mass spectrometer is instructed to perform multiplexed precursor ion selection on the continuous beam of ions, fragment each of the N−1 precursor ions, and measure the intensities of the product ions, producing N product ion spectra. A heat map is plotted, producing N heat maps. The N product ion spectra are combined into a combined product ion spectrum. A corresponding precursor ion of a peak is identified by finding a heat map of the N heat maps that does not have data for the mass of the peak and determining that a precursor ion of the N precursor ions that is not included in a group that produced the heat map is the corresponding precursor ion.