公开(公告)号：CA2521171A1

公开(公告)日：2004-10-21

申请号：CA2521171

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： NASSEF HANY RAMEZ ,  UNGER MARC ,  FACER GEOFFREY ,  MCBRIDE LINCOLN ,  LUCERO MICHAEL

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10 ,  B01L11/00

Abstract: A variety of elastomeric-based microfluidic devices and methods for using an d manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagent s have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and ge ne expression analyses.

公开(公告)号：AU2004228678A1

公开(公告)日：2004-10-21

申请号：AU2004228678

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： UNGER MARC ,  MCBRIDE LINCOLN ,  FACER GEOFFREY ,  LUCERO MICHAEL ,  NASSEF HANY RAMEZ

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.