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公开(公告)号：DE10108240A1
公开(公告)日：2002-08-22
申请号：DE10108240
申请日：2001-02-21
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK , NICKEL JOCHEN
Abstract: Method for imaging and measuring microscopic 3-D structures has the following steps: representation of a data set in 3-D form on a microscope display (27); entry of one or more intersection points and a chosen rotation angle; rotation of the 3-D representation of the display until a 3-D structure is displayed as desired by a user; and subsequent analysis of the structure. Windows (40, 42, 44) on the display are used for setting rotation view, coordinates, rotational angle and intersection point, while cross hairs (70) are to interactively change the position of the intersection point. An Independent claim is made for an arrangement for imaging and measurement of microscopic 3-D structures, using a microscope, microscope display (27), a PC and a PC input device.
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公开(公告)号：DE10065783A1
公开(公告)日：2002-07-11
申请号：DE10065783
申请日：2000-12-30
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: Method for determining process variables has a number of steps. Accordingly multiple intensities are captured and determined by detectors. Said intensities are derived from different spectral ranges of the measurement process and formed together in an intensity vector. A normalized vector is calculated from the intensity vector that is smaller than a given threshold. The individual intensity vectors are then normalized and are processed in a vector quantizer (58). Lastly code-book vectors are read out from the quantizer. The invention also relates to a corresponding system and arrangement for implementation of the method.
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公开(公告)号：DE10234404B4
公开(公告)日：2021-10-14
申请号：DE10234404
申请日：2002-07-29
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: Verfahren zur Überwachung und Kontrolle eines Mikroskops (100, 102), gekennzeichnet durch die folgenden Schritte:a) Ermitteln des Informationsinhaltes mindestens eines Bildes;b) Analyse des Informationsinhalts mit einem vorgegebenen Sollinformationsinhalt und einer vorgegebenen Variation des Informationsinhalts als Toleranzmaß;c) Bestimmen einer Stellgröße aus der Analyse des Informationsinhalts mit einem vorbestimmten Sollwert zur Beeinflussung des Informationsinhalts, um den Informationsinhalt während der Bildaufnahme konstant zu halten;d) Übergeben der Stellgröße an mindestens einen Aktor des Mikroskops; unde) Ausgeben eines Warnsignals bei Variationen des Informationsinhalts außerhalb des Toleranzmaßes.
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公开(公告)号：DE10355150B4
公开(公告)日：2021-01-14
申请号：DE10355150
申请日：2003-11-26
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: Verfahren zur Analyse von Co-Lokalisationen von in einem Objekt (15) vorhandenen Farbstoffen, gekennzeichnet durch die folgenden Schritte:- Bestimmen der Fluoreszenzspektren der aller im Objekt (15) vorhandenen Farbstoffe;- Angabe eines Toleranzbereichs um das Fluoreszenzspektrum des jeweils einzelnen Farbstoffs;- pixelweises Aufnehmen von Spektren des Objekts, in dem sich mindestens zwei Farbstoffe befinden;- Berechnen der Menge der aufgenommenen Spektren innerhalb des Toleranzbereichs um das Fluoreszenzspektrum;- Ermitteln eines Lambda-Vektors für jedes aufgenommene Pixel;- Bestimmen der Zuordnung eines jeden Pixels zum Spektrum;- Ausgabe eines aus den einzelnen Pixeln zusammengesetzten Bilds für jedes Spektrum; und- Ausgabe mindestens eines Bildes für Co-Lokalisationen.
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公开(公告)号：DE10134328B4
公开(公告)日：2012-10-04
申请号：DE10134328
申请日：2001-07-14
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: Verfahren zur Einstellung der Bilderfassung eines Mikroskops (2) mit einer einem Bilddatenerfassungselement (4) zugeordneten Steuerelektronik (6) und einem Computer (14) zum Steuern der Bilderfassung des Mikroskops (2) und zum Verarbeiten der Bilddaten vom Mikroskop (2), gekennzeichnet durch die folgenden Schritte: • Übertragung der Bilddaten vom Bilddatenerfassungselement (4) an ein Speicherelement (10); • Übergabe von Mikroskop-Steuerparametern an einen Kodierer (20), • Kodieren der Bilddaten aus dem Speicherelement (10) vor der Übertragung der Bilddaten an den Computer (14), wobei der Kodierer (20) in Abhängigkeit von den Mikroskop-Steuerparametern zwischen verschiedenen Kodierungen wechselt, und • Verarbeiten der Bilddaten durch den Computer (14).
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Patent Agency Ranking
公开(公告)号：DE10065783B4
公开(公告)日：2007-05-03
申请号：DE10065783
申请日：2000-12-30
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: Method for determining process variables has a number of steps. Accordingly multiple intensities are captured and determined by detectors. Said intensities are derived from different spectral ranges of the measurement process and formed together in an intensity vector. A normalized vector is calculated from the intensity vector that is smaller than a given threshold. The individual intensity vectors are then normalized and are processed in a vector quantizer (58). Lastly code-book vectors are read out from the quantizer. The invention also relates to a corresponding system and arrangement for implementation of the method.
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公开(公告)号：DE10339311B4
公开(公告)日：2006-04-27
申请号：DE10339311
申请日：2003-08-27
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: A system and a method for setting a fluorescence spectrum measurement system for microscopy is disclosed. Using illuminating light ( 3 ) from at least one laser that emits light of one wavelength, a continuous wavelength region is generated. Dyes are stored, with the pertinent excitation and emission spectra, in a database of a computer system ( 23 ). For each dye present in the specimen ( 15 ), a band of the illuminating light ( 3 ) and a band of the detected light ( 17 ) are calculated, the excitation and emission spectra read out from the database being employed. Setting of the calculated band in the illuminating light and in the detected light is performed on the basis of the calculation. Lastly, data acquisition is accomplished with the spectral microscope ( 100 ).
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公开(公告)号：DE102004004115A1
公开(公告)日：2005-08-11
申请号：DE102004004115
申请日：2004-01-28
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK , NICKEL JOCHEN , HAY WILLIAM C
Abstract: A microscope system includes at least one lens that defines an illumination field and at least one light source that emits an illuminating light beam for illuminating a specimen through the lens. At least one detector is provided for, pixel-by-pixel, detecting a detection light beam coming from the specimen. An electronic circuit is connected downstream from the detector, the electronic circuit including a memory unit for storing a wavelength-dependent brightness distribution of an illumination field of the at least one lens. The electronic circuit employs, pixel-by-pixel, the stored wavelength-dependent brightness distribution so as to form a homogeneously illuminated image field. An actuatable element is provided for controlling, pixel-by-pixel, an intensity of the illuminating light beam as a function of the stored wavelength-dependent brightness distribution so as to homogeneously illuminate the illumination field.
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公开(公告)号：DE10339311A1
公开(公告)日：2005-04-14
申请号：DE10339311
申请日：2003-08-27
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: A system and a method for setting a fluorescence spectrum measurement system for microscopy is disclosed. Using illuminating light ( 3 ) from at least one laser that emits light of one wavelength, a continuous wavelength region is generated. Dyes are stored, with the pertinent excitation and emission spectra, in a database of a computer system ( 23 ). For each dye present in the specimen ( 15 ), a band of the illuminating light ( 3 ) and a band of the detected light ( 17 ) are calculated, the excitation and emission spectra read out from the database being employed. Setting of the calculated band in the illuminating light and in the detected light is performed on the basis of the calculation. Lastly, data acquisition is accomplished with the spectral microscope ( 100 ).
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公开(公告)号：DE10339312A1
公开(公告)日：2005-03-31
申请号：DE10339312
申请日：2003-08-27
Applicant: LEICA MICROSYSTEMS
Inventor： OLSCHEWSKI FRANK
Abstract: A method for separating fluorescence spectra of dyes present in a specimen ( 15 ) is disclosed. Firstly a spectral scan of the fluorescence spectrum of all the dyes present in the specimen ( 15 ) is performed. The fluorescence spectra associated with the respective dyes are stored in a database of the computer system. After spectral deconvolution of the acquired mixed fluorescence spectrum, a comparison is made between the measured individual spectra ascertained by spectral deconvolution and the fluorescence spectra associated with the respective dyes. Lastly, a linear deconvolution of the acquired mixed fluorescence spectrum is performed.
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