公开(公告)号：US09964515B2

公开(公告)日：2018-05-08

申请号：US14862930

申请日：2015-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  James Bustillo ,  Mark James Milgrew ,  Jonathan Schultz ,  David Marran ,  Todd Rearick ,  Kim L. Johnson

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

公开(公告)号：US09891190B2

公开(公告)日：2018-02-13

申请号：US15194321

申请日：2016-06-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Matthew Mauro ,  Shifeng Li ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/68 ,  G01N27/26 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

公开(公告)号：US09528962B2

公开(公告)日：2016-12-27

申请号：US14863173

申请日：2015-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shifeng Li ,  James Bustillo ,  Wolfgang Hinz

IPC: B01L3/00 ,  G01N27/414 ,  G01N27/27 ,  H01L21/302 ,  H01L21/31 ,  G01N27/26

CPC classification number: G01N27/4148 ,  B01L3/5027 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0893 ,  G01N27/26 ,  G01N27/27 ,  G01N27/4145 ,  H01L21/302 ,  H01L21/31 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/143333

Abstract: A system includes a sensor including a sensor pad and includes a well wall structure defining a well operatively connected to the sensor pad. The sensor pad is associated with a lower surface of the well. The well wall structure defines an upper surface and a wall surface extending between the upper surface and the lower surface. The upper surface is defined by an upper buffer material having an intrinsic buffer capacity of at least 2×1017 groups/m2. The wall surface is defined by a wall material having an intrinsic buffer capacity of not greater than 1.7×1017 groups/m2.

公开(公告)号：US08841217B1

公开(公告)日：2014-09-23

申请号：US13801243

申请日：2013-03-13

Applicant: Life Technologies Corporation

Inventor： Keith Fife ,  James Bustillo ,  Jordan Owens

IPC: H01L21/311 ,  G01N27/414

CPC classification number: G01N27/414

Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive element on a sidewall of the opening and extending over an upper surface of the dielectric material.

Abstract translation: 在一个实施方式中，描述了化学传感器。 化学传感器包括具有上表面的浮栅导体的化学敏感场效应晶体管。 介电材料限定了延伸到浮动栅极导体的上表面的开口。 在开口的侧壁上并在电介质材料的上表面上延伸的导电元件。

公开(公告)号：US08647577B2

公开(公告)日：2014-02-11

申请号：US13648663

申请日：2012-10-10

Applicant: Life Technologies Corporation

Inventor： Wolfgang Hinz ,  John Matthew Mauro ,  Shifeng Li ,  James Bustillo

IPC: G01N27/00 ,  G01N27/30 ,  B05D5/12 ,  B28B19/00 ,  B29B15/10 ,  C23C18/00 ,  C23C20/00 ,  C23C24/00 ,  C23C26/00 ,  C23C28/00 ,  C23C30/00 ,  H01C17/06 ,  H05K3/00 ,  H01L21/00 ,  G01N30/96

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

Abstract translation: 所描述的实施例可以提供一种制造化学检测装置的方法。 该方法可以包括在CMOS器件上形成微孔。 微孔可以包括底表面和侧壁。 该方法可以进一步包括施加第一化学物质以选择性地连接到微孔的底表面，在微孔的侧壁上形成金属氧化物层，以及施加第二化学物质以选择性地连接到微孔的侧壁。 第二种化学物质可能与第一种化学物质不具有亲和力。

公开(公告)号：US12066399B2

公开(公告)日：2024-08-20

申请号：US18353017

申请日：2023-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/423 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/78 ,  H01L29/42324 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US11874250B2

公开(公告)日：2024-01-16

申请号：US17823696

申请日：2022-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber ,  Mark James Milgrew ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q1/6874 ,  C12Q2565/607

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11448613B2

公开(公告)日：2022-09-20

申请号：US16101337

申请日：2018-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  John F. Davidson ,  Antoine M. van Oijen ,  John Leamon ,  Martin Huber ,  Mark James Milgrew ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L21/82 ,  C12Q1/6869

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11339430B2

公开(公告)日：2022-05-24

申请号：US16846113

申请日：2020-04-10

Applicant: Life Technologies Corporation

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: C12Q1/6869 ,  H01L23/00 ,  H01L29/78 ,  G01N27/414 ,  C12Q1/6874 ,  H01L21/306 ,  H01L27/088

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US20200378919A1

公开(公告)日：2020-12-03

申请号：US16924055

申请日：2020-07-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark James MILGREW ,  Jonathan M. Rothberg ,  James Bustillo

IPC: G01N27/414 ,  G01N27/27 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.