公开(公告)号：JPS5855455A

公开(公告)日：1983-04-01

申请号：JP15302581

申请日：1981-09-29

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  FUJIMURA SUSUMU ,  YOKOO YASUHIKO

IPC: A01N37/32 ,  A01N37/34 ,  C07D207/456

Abstract: NEW MATERIAL:The aminomaleimide of formulaI[R is H, 1-4C alkyl, or group of formula II (R is 1-4C alkyl or halogen; m is 0-3); X is 2-5C alkoxycarbonyl, 2-5C aliphatic acyl, benzoyl, or cyano]. EXAMPLE:3-Amino-4-cyanomaleimide. USE:Useful as pharmaceuticals, pesticides and their intermediates. PROCESS:The compound of formulaIcan be prepared by reacting a cyanoformic acid ester of formula III (R is 1-4C alkyl) with an amide of formula IV in the presence of zinc chloride and a tert-amine preferably in a solvent such as benzene, methylene chloride, etc. at 20-100 deg.C, and treating the resultant reaction product with an acidic aqueous solution such as hydrochloric acid, p-toleunesulfonic acid, etc.

公开(公告)号：JPS5846059A

公开(公告)日：1983-03-17

申请号：JP14376981

申请日：1981-09-14

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  OKIMOTO KIYOMI

IPC: C07C257/14 ,  C07C67/00 ,  C07C239/00

Abstract: NEW MATERIAL:A compound of formulaI[R is 1-4C alkyl, 1-4C fluorinated alkyl, halogen, etc.; R is 1-10C alkyl, 2-4C alkenyl, 5-7C cycloalkyl, etc.; (n) is 0, 1, 2 or 3]. EXAMPLE:N-phenylamidino-N-cyclohexylcarboxamide. USE:Agricultural chemicals, medicines and intermediates therefor. PROCESS:An alkoxyiminoacetic ester of formula II (R and R are 1-4C alkyl) is reacted with an aromatic amine in the presence or absence of a reaction solvent at 20-150 deg.C to give a compound of formula V, which is then reacted with an amine of formula IV to afford the compound of formulaI.

公开(公告)号：JPS53141290A

公开(公告)日：1978-12-08

申请号：JP5439477

申请日：1977-05-13

Applicant: UBE INDUSTRIES

Inventor： MAZAKI MITSUO ,  FUKUI KIYOSHI ,  KITA JIYUNICHIROU

IPC: C07D201/02

Abstract: PURPOSE:To prepare ?-caprolactam in high purity and high yield without formation of ammonium sulfate by hydrogenation of 1,3-dihydro-2H-azepin-2-ones in the presence of a cheap nickel catalyst.

公开(公告)号：JPH06217768A

公开(公告)日：1994-08-09

申请号：JP1097993

申请日：1993-01-26

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KUBO FUMIO ,  KUDO ISAO ,  UMEDA KAZUHIRO ,  IWAMOTO YOSHITADA ,  OKA YURIO

IPC: C12N9/02 ,  C12R1/19

Abstract: PURPOSE:To produce human copper, zinc type super oxide dismutase containing no copper or deficient in copper and useful as a medicine by culturing a microbial cell having human copper, zinc type super oxide dismutase-producing ability. CONSTITUTION:Yield of human copper and zinc type super oxide dismutase containing no copper or deficient in copper to microbial cell amount can remarkably be enhanced by adding Escherichia coli having human copper, zinc type super oxide dismutase producing ability to 90,000ml medium containing K2HPO4, KH2PO4, Na2HPO4, soybean protein hydrolyzate, methionine, tryptophan, aspartic acid, glucose, MgSO4, CaCl2.2H2O, MnSO4, AlCl3, H3BO3, FeSO4, CuCl2.2H2O, ZnSO4.7H2O and tetracycline and culturing the bacterium while adding 10.71g ZnSO4.7H2O thereto in three portions during culture.

公开(公告)号：JPH01235591A

公开(公告)日：1989-09-20

申请号：JP5952788

申请日：1988-03-15

Applicant: UBE INDUSTRIES ,  NIPPON SEKIJUJISHA

Inventor： SEKIGUCHI SADAMI ,  ITO KEIZO ,  FUKUI KIYOSHI ,  WATANABE MASAYUKI

IPC: C12N9/08

Abstract: PURPOSE:To mass produce catalase in high yield and in high purity, by treating an aqueous solution containing both catalase and accompanying protein by chromatography of hydrophobic mutual action. CONSTITUTION:A blood cell, etc., is mixed with equivalent distilled water, hemolyzed and hemoglobin is removed to give an aqueous solution (A) containing catalase and protein. Then a functional group such as phenyl Sepharose CL-4B is supported on a carrier such as cellulose to give a chromatography resin (B) of hydrophobic mutual action. Then the resin B is equilibrated with 5-10 times as much a buffer solution (C) having a given pH as the resin, the solution A is adjusted in such a way that total protein in the solution A is

公开(公告)号：JPH01235590A

公开(公告)日：1989-09-20

申请号：JP5952888

申请日：1988-03-15

Applicant: UBE INDUSTRIES ,  NIPPON SEKIJUJISHA

Inventor： SEKIGUCHI SADAMI ,  ITO KEIZO ,  FUKUI KIYOSHI ,  WATANABE MASAYUKI

IPC: C12N9/02 ,  C12N9/08

Abstract: PURPOSE:To separate and to purify a large amount of the title superoxide dismutase and catalase simply and in high purity, by treating an hemolyzed solution with an anion exchanger equilibrated with a phosphoric acid buffer solution at a given pH. CONSTITUTION:A hemolyzed solution of human, etc. is treated with an anion exchanger equilibrated with a phosphoric acid buffer solution at pH 7.2-7.5 and both Cu, Zn type superoxide dismutase (SOD) and catalase are recovered to give a recovered material (A). Then the component A is concentrated, desalted, SOD and catalase as the same fraction are purified by anion exchange chromatography to give a purified fraction (B). Then the component B is passed through a metallic chelating affinity chromatography resin and separated into SOD fraction (C) and a catalase fraction (D). Then the fraction C is purified in the presence of a thiosulfate by chromatography carrying copper, the fraction D is purified chromatography of hydrophobic mutual action to recover erythrocyte derived Cu, Zn type superoxide dismutase and catalase.

公开(公告)号：JPH01235588A

公开(公告)日：1989-09-20

申请号：JP5952588

申请日：1988-03-15

Applicant: UBE INDUSTRIES ,  NIPPON SEKIJUJISHA

Inventor： SEKIGUCHI SADAMI ,  ITO KEIZO ,  FUKUI KIYOSHI ,  WATANABE MASAYUKI

IPC: C12N9/02 ,  C12N9/08

Abstract: PURPOSE:To recover the title superoxide dismutase and catalase in high purity, in high yield and in a short time, by treating hemolyzed solution with an anion exchanger in the presence of a nonionic surfactant. CONSTITUTION:A blood cell fraction obtained from human blood, etc. is hemolyzed with an equal volume of distilled water to give a hemolyzed solution (A). Then an exchange group such as tertiary amine of diethylamino ethyl group, etc. is bonded to a carrier such as cellulose, etc. to give an anion exchanger (B). Then 0.1-1l component B is added to 1l solution A, which is mixed with 0.05-0.5W/V% nonionic surfactant (C) (e.g. polyoxyethylene decyl ether) equilibratged with a buffer solution at pH 6.5-7 stirred for 1-6hr, filtered under suction to give an exchanger (D) having adsorbed SOD (superoxide dismutase) and catalase. Then the component D is eluted with a buffer solution at pH 6-7 to recover erythrocyte derived Cu, Zn type speroxide dismutase and catalase.

公开(公告)号：JPS60218365A

公开(公告)日：1985-11-01

申请号：JP7290784

申请日：1984-04-13

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU

IPC: C07C257/14 ,  C07C67/00 ,  C07C239/00

Abstract: NEW MATERIAL:A compound of formula I (R is 1-6C alkyl; R is 1-6C alkyl or benzyl; R and R may mutually link or through O form a ring when R is alkyl). EXAMPLE:N,N-Oxydiethyleneamidinecarboxamide. USE:Useful as an agricultural chemical, medicine and intermediate therefor. PREPARATION:An alkoxyiminoacetamide of formula II (R is lower alkyl) is reacted with a secondary amine of formula III in the presence of a solvent, e.g. benzene, at 20-100 deg.C for 1-100hr to give the aimed compound of formula I .

公开(公告)号：JPS60181073A

公开(公告)日：1985-09-14

申请号：JP3535884

申请日：1984-02-28

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KAKEYA NOBORU ,  MATSUO FUMIO

IPC: C07D233/96

Abstract: NEW MATERIAL:The 5-carbamoylfluoromethylidenehydantoin of formula I (R is H, alkyl or allyl). EXAMPLE:5-Carbamoylfluoromethylidenehydantoin. USE:Pharmaceuticals, agricultural chemicals or their raw materials. PREPARATION:The compound of formula I can be prepared by reacting 1mol of 5-(alkyoxycarbonyl)fluoromethylidenehydantoin of formula II (R is lower alkyl) with 3-20mol of the amine of formula R -NH2 such as ammonia, methylamine, etc. in the presence of water at room temperature for

公开(公告)号：JPS5865273A

公开(公告)日：1983-04-18

申请号：JP16098581

申请日：1981-10-12

Applicant: UBE INDUSTRIES

Inventor： FUKUI KIYOSHI ,  KITA JIYUNICHIROU ,  FUJIMURA SUSUMU

IPC: A01N47/36 ,  A01N37/32 ,  C07D207/456

Abstract: NEW MATERIAL:The compound of formulaI[R is 1-4C alkyl or halogen; m is 0-3; R is 2-5C alkoxycarbonyl or benzoyl; X is amino, -NHR (R is 1-4C alkyl, 2-4C alkenyl, 5-7C cycloalkyl, 7-10C aralkyl, etc.), or -N(CH2)p (p is 4-6)]. EXAMPLE:3-Ethoxycarbonyl-1-phenyl-4-(phenylcarbamoyl)aminomaleimide. USE:Pharmaceuticals, pesticides and their intermediate. PROCESS:The compound of formulaIcan be prepared by reacting the amino- maleimide of formula II with phosgene or trichloromethyl chloroformate preferably in an organic solvent in the presence of pyridine at -20-+30 deg.C for 0.5- 5hr, and reacting the resultant compound with the amine of formula X-H at -20-+100 deg.C for 1-20hrs.