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    Expression control using antibody expression optimisation sequences
    32.
    发明专利
    Expression control using antibody expression optimisation sequences 未知

    公开(公告)号：AU2006298590A1

    公开(公告)日：2007-04-12

    申请号：AU2006298590

    申请日：2006-09-29

    Applicant: UCB PHARMA SA

    Inventor： HUMPHREYS DAVID PAUL ,  ELLIS MARK

    IPC: C07K16/00 ,  C07K16/24 ,  C07K16/28 ,  C12N15/72

    Abstract: The present invention provides a dicistronic message for producing an antibody molecule, in which the upstream cistron contains DNA coding for the light chain of the antibody and the downstream cistron contains DNA coding for the corresponding heavy chain, characterised in that the dicistronic message comprises a sequence selected from AEOS1 (SEQ ID NO:1), AEOS2 (SEQ ID NO:2), AEOS3 (SEQ ID NO:3), AEOS4 (SEQ ID NO: 4), AEOS5 (SEQ ID NO:5), AEOS6 (SEQ ID NO:6), AEOS7 (SEQ ID NO:7), AEOS8 (SEQ ID NO:8), AEOS9 (SEQ ID NO:9), AEOS1O (SEQ ID NO:10) and AEOS11 (SEQ ID NO:11).

    BACTERIAL HOST STRAIN EXPRESSING RECOMBINANT DSBC
    33.
    发明专利
    BACTERIAL HOST STRAIN EXPRESSING RECOMBINANT DSBC 未知

    公开(公告)号：CA2841824C

    公开(公告)日：2021-07-13

    申请号：CA2841824

    申请日：2012-07-13

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    IPC: C07K16/28 ,  C12N15/70

    Abstract: The present invention relates to the development of a bacterial periplasmic system for expressing antibodies. Specifically there is provided a recombinant gram-negative bacterial cell comprising: a mutated Tsp gene which is a knockout mutated Tsp gene or which encodes a Tsp protein having reduced protease activity compared to a wild type Tsp gene, a mutated spr gene encoding an spr protein capable of suppressing the growth phenotype of a cell comprising a mutated Tsp phenotype, an expression vector comprising a recombinant polynucleotide encoding DsbC; and one or more polynucleotides encoding an antibody or an antigen binding fragment thereof specifically binding to CD154.

    BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES
    34.
    发明专利
    BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES 未知

    公开(公告)号：CA2774692C

    公开(公告)日：2017-12-19

    申请号：CA2774692

    申请日：2010-09-23

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    IPC: C12N9/50 ,  C12P21/00

    Abstract: A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.

    cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida
    37.
    发明专利
    cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida 未知

    公开(公告)号：BR112012017425A2

    公开(公告)日：2015-09-15

    申请号：BR112012017425

    申请日：2011-01-13

    Applicant: UCB PHARMA SA

    Inventor： HUMPHREYS DAVID PAUL ,  ELLIS MARK

    IPC: C07K14/245 ,  C12N15/70

    Abstract: cepa hospedeira bacteriana compreendendo um gene spr mutante e tendo atividade tsp reduzida. a presente invenção fornece uma célula bacteriana gram negativa recombinante que compreende um gene spr mutante que codifica uma proteína spr tendo uma mutação em um ou mais aminoácidos selecionados de d133, h145, h157, n31, r62, i70, q73, c94,s95, v98, r100, l108, y115, v135, l136, g140, r144 e g147 e em que a célula possui atividade de proteína tsp reduzida comparada com uma célula do tipo selvagem.

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    38.
    发明专利
    "BACTERIAL HOST STRAIN" 未知

    公开(公告)号：IN2401DEN2012A

    公开(公告)日：2015-08-21

    申请号：IN2401DEN2012

    申请日：2012-03-20

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    IPC: C12N9/50

    Abstract: A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity ; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.

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