公开(公告)号：KR1020030075163A

公开(公告)日：2003-09-22

申请号：KR1020037009544

申请日：2002-01-18

Applicant: 바스프 에스이

Inventor： 요쿰,알.,로저스 ,  패터슨,토마스,에이. ,  페로,제니스,쥐. ,  헤르만,테론

IPC: C12P13/02

CPC classification number: C12N9/0006 ,  C12N9/1014 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/93 ,  C12P13/02 ,  C12Y201/02011 ,  C12Y401/01011 ,  C12Y603/02001

Abstract: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

Abstract translation: 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加关键中间体水平，通过有助于其合成的酶的酮托品来提高所需产物的生产的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

公开(公告)号：KR100832740B1

公开(公告)日：2008-05-27

申请号：KR1020070005268

申请日：2007-01-17

Applicant: 한국과학기술원

Inventor： 이상엽 ,  박진환 ,  이광호 ,  김태용

IPC: C12N1/16 ,  C12N1/14 ,  C12P13/08

CPC classification number: C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

Abstract: A mutant microorganism obtained by mutation and metabolic flux operation is provided to produce a branched amino acid, particularly L-valine with high efficiency, thereby being industrially useful for generating the L-valine. A method for preparing a mutant microorganism with high L-valine productivity is characterized in that a gene encoding an enzyme involving with L-isoleucine bio-synthesis such as ilvA(a gene encoding threonine dehydratase), a gene encoding an enzyme involving with L-leucine bio-synthesis such as leuA(a gene encoding 2-isopropylmalate synthase), and a gene encoding an enzyme involving with D-pantothenic acid bio-synthesis such as panB(a gene encoding 3-methyl-2-oxobutanoate hydroxymethyl transferase) are attenuated or deleted and a gene encoding an enzyme involving with L-valine bio-synthesis is mutated by deleting lacI(a gene encoding lac operon repressor), removing feedback inhibition of ilvH(acetohydroxy acid synthase isozyme III) gene, substituting a native promoter including an attenuator of ilvGMEDA(acetohydroxy acid synthase isozyme I) and ilvBN(acetohydroxy acid synthase isozyme II) with a strong promoter, or introducing an expression vector including a strong promoter. A method for preparing L-valine comprises the steps of: (a) culturing the mutant microorganism having high L-valine productivity; and (b) recovering the L-valine from a culture solution of the microorganism.

Abstract translation: 提供通过突变和代谢通量操作获得的突变体微生物以高效率产生支链氨基酸，特别是L-缬氨酸，从而在工业上可用于产生L-缬氨酸。 制备具有高L-缬氨酸生产力的突变型微生物的方法的特征在于编码涉及L-异亮氨酸生物合成的酶如ilvA（编码苏氨酸脱水酶的基因）的基因，编码涉及L-缬氨酸的酶的基因， 亮氨酸生物合成如leuA（编码2-异丙基苹果酸合酶的基因）和编码涉及D-泛酸生物合成的酶如panB（编码3-甲基-2-氧代丁酸羟甲酯转移酶的基因）的基因的基因是 减毒或缺失，并且通过缺失lacI（编码lac操纵子阻遏物的基因）来突变编码涉及L-缬氨酸生物合成的酶的基因，去除ilvH（乙酰羟酸合酶同功酶III）基因的反馈抑制，用天然启动子取代，包括 具有强启动子的ilvGMEDA（乙酰羟酸合酶同功酶I）和ilvBN（乙酰羟酸合酶同功酶II）的衰减剂，或引入包含强启动子的表达载体。 制备L-缬氨酸的方法包括以下步骤：（a）培养具有高L-缬氨酸生产力的突变微生物; 和（b）从微生物的培养液中回收L-缬氨酸。