Abstract:
An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
Abstract:
Apparatus and process for facilitating a full-field microscopic imaging of at least one anatomical structure can be provided. For example, with an interferometric arrangement, it is possible (i) receive a first radiation from a reference arm and a second radiation from the anatomical structure(s), and (ii) generate a third radiation. For example, the first radiation and/or the second radiation can be modulated, and the third radiation can be a combination of the first and second radiations. Further, a single -pixel photodetector or multiple photodetectors arrangement can be used to receive and detect the third radiation for facilitating the full-field microscopic imaging of the anatomical structure(s).
Abstract:
An optical analysis system and process are disclosed. The optical analysis system includes one or more optical filter mechanisms disposed to receive light from a light source and a detector mechanism configured for operative communication with the one or more optical filter mechanisms, the operative communication permitting measurement of properties of filtered light, filtered by the one or more optical filter mechanisms followed by optical filtering by the mosaic optical filter mechanism from the light received. The one or more optical filter mechanisms are configured so that the magnitude of the properties measured by the detector mechanism is proportional to information carried by the filtered light. The process uses the optical analysis system.