Abstract:
A manufacturing method of the transformed reproduction goat producing hgm-csf and transformation reproduction goat produced by the method are provided. A transformation reproduction goat manufacturing method for producing the CSF protein comprises: a step for separating the somatic cell from the fetus of goat or the female goat; a step for introducing into the somatic cell after preparing expression vector including the polynucleotide coding CSF; a step for cultivating after selecting the clone somatic cell in which the expression vector is introduced; a step for removing the nucleus of the ovum collected from the mother animal goat and fusing with the clone somatic cell; and a step for transplanting the fused clone egg into the goat.
Abstract:
A production method of a transgenic cloned embryo having a goat somatic cell nucleus transfected with hGM-CSF(human granulocyte macrophage-colony stimulating factor) gene is provided to produce a cloned goat expressing the hGM-CSF from the mammary gland, thereby improving the production yield of hGM-CSF. A production method of a transgenic cloned goat embryo comprises the steps of: (1) transforming a somatic cell of goat with a recombinant expression vector containing an hGM-CSF gene to prepare a nucleus donor cell; (2) collecting an ovum from the goat to prepare a nucleus recipient ovum; and (3) denucleating the recipient ovum, transplanting the nucleus donor cell into the recipient ovum and fusing them to prepare the transgenic cloned embryo.
Abstract:
A method for producing transgenic pig capable of expressing colony stimulating factor(CSF) gene in the mammary gland is provided to be able to easily obtain the transgenic pig, which is able to solve the problems of post-translational modification or mass-production and is used as a supplemental agent of treating leukemia and an immuno-potentiating agent, by expressing hGM-CSF in mammary gland. The method comprises the steps of: (a) isolating somatic cell from pig fetus on days 40-80 pregnancy; (b) after preparing an expression vector including a promoter specific to mammary gland such as alphas1-casein, beta-casein, beta-lactoglobulin and whey acidic protein; and a polynucleotide encoding the CSF such as hGM-CSF, introducing it into the somatic cell; (c) after selecting the expression vector-introduced clone somatic cell, culturing it; (d) removing nucleus of ovum collected from sow and fusing it with the clone somatic cell; and (e) transplanting the fused nuclear transfer embryos to surrogate mother pig and delivering piglets.
Abstract:
프라이버시 보호 서비스를 제공하는 메시지 서버의 동작 방법이 개시된다. 일 실시예는 전자 메시지의 송신 요청에 포함된 데이터 필드 중 적어도 하나의 원소를 추출하고, 추출된 적어도 하나의 원소에 기초하여 프라이버시 데이터를 생성하며, 프라이버시 데이터를 미리 구비된 데이터베이스에 저장하고, 프라이버시 데이터에 접근하기 위한 링크를 생성하는 단계들을 포함한다.
Abstract:
Provided is a method for providing an exercise prescription based on a network comprising a step for receiving a set of information related to a gesture and an operation of a user measured from a measuring device; a step for extracting at least one exercise prescription from a database or an expert pool system storing a plurality of predetermined exercise prescriptions based on the information related to the gesture and the operation of the user; a step for receiving feedback for a performance result of the extracted exercise prescription; and a step for updating the database by using the performance result. [Reference numerals] (150) Expert pool system
Abstract:
PURPOSE: A method for developing, verifying and providing robot contents and application using a cloud platform and a system thereof are provided to provide optimum application and robot contents to a target robot, by selecting and providing the application and the robot contents corresponding to the robot characteristics and hardware characteristics of the target robot. CONSTITUTION: An editor (210) manufactures application and contents for a target robot (240), and a verification tool and simulator (230) verifies the manufactured application and the manufactured robot contents. A cloud platform (220) provides library related with the manufactured application to the verification tool, and provides operation environment for the manufactured application and the manufactured contents to the simulator. The target robot downloads the selected application and the selected robot contents from an app store (250) on the basis of the robot characteristics and the hardware characteristics of the target robot. [Reference numerals] (210) Editor; (220) Cloud platform; (233) Testing and verifying tool; (236) Simulator; (250) App Store; (AA) Upload
Abstract:
PURPOSE: A foot image photographing device is provided to produce balance information about people based on pressure on the front and back sides or the left and right sides of a foot. CONSTITUTION: A foot (10) is located on a transparent footstool (100). A first reflection plate (101) is installed on the upper surface of the transparent footstool at a preset angle. The first reflection plate reflects the rear image of the foot which is located on the upper surface of the transparent footstool to the transparent footstool. A second reflection plate (102) is installed on the lower surface of the transparent footstool at the preset angle and reflects an image projected through the footstool. A camera (103) photographs the image which is reflected by the second reflection plate. The image which is photographed by the camera includes a bottom image and the rear image.