公开(公告)号：EP1446669A1

公开(公告)日：2004-08-18

申请号：EP02786552.6

申请日：2002-10-28

Applicant: Advion BioSciences, Inc.

Inventor： SCHULTZ, Gary, A. ,  SHELDON, Gary, S. ,  JUNG, David, R. ,  ALPHA, Christopher, G. ,  RULE, Geoffrey, S. ,  HUANG, Xian ,  PROSSER, Simon, J.

IPC: G01N35/02 ,  G01N1/00 ,  G01N1/16 ,  G01N1/04 ,  G01N1/10 ,  G01N21/00 ,  G01N31/22 ,  G01N33/00 ,  G01N15/06 ,  G01N30/02 ,  B01L3/00 ,  B01L3/02 ,  B01L11/00 ,  B01L9/00

CPC classification number: G01N30/6034 ,  B01L3/0275 ,  B01L9/06 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/142 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0832 ,  G01N30/24 ,  G01N30/463 ,  G01N30/466 ,  G01N30/468 ,  G01N30/6004 ,  G01N30/6039 ,  G01N30/6043 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6091 ,  G01N30/7233 ,  G01N2030/8881 ,  G01N2035/00237 ,  G01N2035/00326 ,  Y10T436/25 ,  Y10T436/255 ,  Y10T436/2575

Abstract: A disposable tube (80) includes an inlet end (81) and an outlet end (82), wherein the inlet end of a first tube is self-locking, self-aligning, self-mating, self-sealing and adapted to detachably engage an outlet end of a second tube. The tube may be filled with separation material. The tubes may be used for micro fluidic separation and fluid transfer. Also included is a tube array having a plurality of tube holders adjacent to one another. Each tube holder has a passageway configured to receive a tube. Each passageway constrains the movement of a tube in the array: allowing free movement of the tube along the tube axis, while allowing limited sideways movement of the tube, so that the tube is held in alignment with a corresponding input port of, for example, a sample transfer device.

公开(公告)号：EP1876443A3

公开(公告)日：2008-03-12

申请号：EP07019029.3

申请日：1999-09-01

Applicant: Advion BioSciences, Inc. ,  KIONIX, INC.

Inventor： Moon, James, E. ,  Schultz, Gary, A. ,  Corso, Thomas, N. ,  Davis, Timothy, J. ,  Galvin, Gregory, J. ,  Lowes, Stephen

IPC: G01N30/60

CPC classification number: G01N30/6095 ,  G01N30/7266 ,  G01N2030/6013 ,  H01J49/0018 ,  H01J49/167 ,  Y10T436/24 ,  Y10T436/2575

Abstract: An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate; and, optionally, electrode(s) for application of a electric potential to the fluid. The exit orifice of the liquid chromatography device may be homogeneously interfaced with the entrance orifice of the electrospray device to form an integrated single system. An array of multiple systems may be fabricated in a single monolithic chip for rapid sequential fluid processing and generation of electrospray for subsequent analysis, such as by positioning the exit orifices of the electrospray devices near the sampling orifice of a mass spectrometer.

Abstract translation: 公开了电喷雾装置，液相色谱装置和电喷雾液相色谱系统。 该电喷射装置包括基板，该基板在注射表面上的入口孔与喷射表面上的出口孔之间限定通道，由从围绕出口孔的喷射表面凹进的部分限定的喷嘴和用于施加电的电极 对底物进行优化和产生电喷雾的潜力; 并且可选地附加电极以进一步修改电喷雾。 所述液相色谱装置包括分离基底，所述分离基底限定入口孔与储存器之间的引入通道以及所述储存器与出口孔之间的分离通道，所述分离通道填充有垂直于所述流体流的分离柱; 粘合到所述分离基板上以覆盖所述储存器和所述分离通道并邻近所述盖基板的盖基板; 以及可选的用于向流体施加电势的电极。 液相色谱装置的出口孔可以与电喷雾装置的入口孔均匀地接合以形成集成的单一系统。 可以在单个单片芯片中制造多个系统的阵列，用于快速顺序流体处理和产生电喷雾用于随后的分析，例如通过将电喷射装置的出口孔定位在质谱仪的取样孔口附近。

公开(公告)号：EP1876444A2

公开(公告)日：2008-01-09

申请号：EP07019028.5

申请日：1999-09-01

Applicant: Advion BioSciences, Inc. ,  KIONIX, INC.

Inventor： Moon, James, E. ,  Schultz, Gary, A ,  Corso, Thomas, N. ,  Davis, Tmothy, J. ,  Galvin, Gregory, J. ,  Lowes, Stephen

IPC: G01N30/72

CPC classification number: G01N30/6095 ,  G01N30/7266 ,  G01N2030/6013 ,  H01J49/0018 ,  H01J49/167 ,  Y10T436/24 ,  Y10T436/2575

Abstract: An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate; and, optionally, electrode(s) for application of a electric potential to the fluid. The exit orifice of the liquid chromatography device may be homogeneously interfaced with the entrance orifice of the electrospray device to form an integrated single system. An array of multiple systems may be fabricated in a single monolithic chip for rapid sequential fluid processing and generation of electrospray for subsequent analysis, such as by positioning the exit orifices of the electrospray devices near the sampling orifice of a mass spectrometer.

公开(公告)号：EP1876443A2

公开(公告)日：2008-01-09

申请号：EP07019029.3

申请日：1999-09-01

Applicant: Advion BioSciences, Inc. ,  KIONIX, INC.

Inventor： Moon, James, E. ,  Schultz, Gary, A. ,  Corso, Thomas, N. ,  Davis, Timothy, J. ,  Galvin, Gregory, J. ,  Lowes, Stephen

IPC: G01N30/60

CPC classification number: G01N30/6095 ,  G01N30/7266 ,  G01N2030/6013 ,  H01J49/0018 ,  H01J49/167 ,  Y10T436/24 ,  Y10T436/2575

Abstract: An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate; and, optionally, electrode(s) for application of a electric potential to the fluid. The exit orifice of the liquid chromatography device may be homogeneously interfaced with the entrance orifice of the electrospray device to form an integrated single system. An array of multiple systems may be fabricated in a single monolithic chip for rapid sequential fluid processing and generation of electrospray for subsequent analysis, such as by positioning the exit orifices of the electrospray devices near the sampling orifice of a mass spectrometer.

Abstract translation: 电喷射设备，液相色谱设备和电喷雾 - 液相色谱系统中游离缺失光盘。 电喷雾装置包括在入口孔的喷射表面上的底物限定的通道之间和到喷射表面的出口孔，通过从围绕出口孔喷射表面凹入的部分限定的喷嘴，并且在电极的电气的应用 电位施加到基片，以优化和产生到电喷雾; 和，任选地，另外的（多个）电极つweiterer修改电。 液相色谱装置包括分离的基板限定之间的入口孔和储存器之间和出口孔的分离通道中的引入通道的贮存器，并且分离通道被填充有分离的帖子垂直于所述流体流动; 结合到分离的基板以封闭所述容器和所述分离通道邻近所述盖基板的盖基板; 和，任选地，（多个）电极为一个电位的流体的应用。 液相色谱装置的出口孔可与电装置的入口孔被均匀地接口到形成在集成的单个系统。多个系统的阵列可在用于电喷射的快速连续的流体处理和生成一个单芯片来制造 随后的分析，颜色：如通过定位附近的质谱仪的取样孔的电设备的出口孔。

公开(公告)号：EP1163512A4

公开(公告)日：2007-08-08

申请号：EP00913655

申请日：2000-02-29

Applicant: ADVION BIOSCIENCES INC ,  KIONIX INC

Inventor： SCHULTZ GARY A ,  CORSO THOMAS N ,  MOON JAMES E ,  DAVIS TIMOTHY J ,  GALVIN GREGORY J

IPC: G01N30/60 ,  G01N30/72 ,  H01J49/04 ,  H01J49/10

CPC classification number: H01J49/167 ,  G01N30/6095 ,  G01N30/7266 ,  H01J49/0018

Abstract: A droplet/electrospray device and a liquid chromatography-electrospray system are disclosed. The droplet/electrospray device (100) comprises a substrate (102) defining a channel (104) between an entrance orifice (106) on an injection surface (108) and an exit orifice on an ejection surface (112), a nozzle (110) defined by a portion recessed from the ejection surface (112) surrounding the exit orifice, and an electrode (122) for application of an electric potential to the substrate (102) to optimize and generate droplets or an electrospray (62). A plurality of these electrospray devices (100) can be used in the form of an array of miniaturized nozzles. The liquid chromatography-electrospray device (160) comprises a separation substrate (162) defining an introduction channel (164) between an entrance orifice and a reservoir (166) and a separation channel (168) between the reservoir (166) and an exit orifice (170), the separation channel (168) being populated with separation posts (174) perpendicular to the fluid flow.

公开(公告)号：EP1113850A4

公开(公告)日：2006-02-01

申请号：EP99945401

申请日：1999-09-01

Applicant: ADVION BIOSCIENCES INC ,  KIONIX INC

Inventor： MOON JAMES E ,  SCHULTZ GARY A ,  CORSO THOMAS N ,  DAVIS TIMOTHY J ,  GALVIN GREGORY J ,  LOWES STEPHEN

IPC: G01N27/62 ,  B01D15/08 ,  G01N30/60 ,  G01N30/72 ,  G01N37/00 ,  H01J49/04

CPC classification number: G01N30/6095 ,  G01N30/7266 ,  G01N2030/6013 ,  H01J49/0018 ,  H01J49/167 ,  Y10T436/24 ,  Y10T436/2575

公开(公告)号：EP1309381A1

公开(公告)日：2003-05-14

申请号：EP01948306.4

申请日：2001-06-11

Applicant: Advion BioSciences, Inc.

Inventor： HUANG, Xian

IPC: B01D15/08

CPC classification number: B01J20/287 ,  B01D15/325 ,  B01J20/26 ,  B01J20/261 ,  B01J20/28097 ,  B01J20/285 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3278 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/82 ,  Y10T428/249987 ,  Y10T428/249988

Abstract: A process is provided for modifying a porous polystyrene monolith to render its internal pore surfaces grafted with alkyl groups, preferably of at least four carbon atoms, which includes the step of alkylating the monolith with a uniform liquid solution containing a Friedel-Crafts catalyst and an alkyl halide. A surface-modified monolith produced thereby is an efficient separation medium in reversed-phase liquid chromatography for both small and large biomolecules.

公开(公告)号：EP1252336A1

公开(公告)日：2002-10-30

申请号：EP01910423.1

申请日：2001-02-02

Applicant: Advion BioSciences, Inc.

Inventor： SCHULTZ, Gary, A. ,  ZHANG, Sheng ,  VAN PELT, Colleen K.

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  B01J2219/00274 ,  C12Q1/6869 ,  H01J49/165 ,  C12Q2565/627

Abstract: The present invention relates to a method of detecting single nucleotide polymorphisms by providing a target nucleic acid molecule, an oligonucleotide primer complementary to a portion of the target nucleic acid molecule, a nucleic acid polymerizing enzyme, and a plurality of types of nucleotide analogs. The target nucleic molecule, the oligonucleotide primer, the nucleic acid polymerizing enzyme, and the nucleotide analogs, each type being present in a first amount, are blended to form an extension solution where the oligonucleotide primer is hybridized to the target nucleic acid molecule to form a primed target nucleic acid molecules and the nucleic acid polymerizing enzyme is positioned to add nucleotide analogs to the prime target nucleic acid molecule at an active site. The oligonucleotide primer in the extension solution is extended by using the nucleic acid polymerizing enzyme to add a nucleotide analog to the oligonucleotide primer at the active site. This forms an extended oligonucleotide primer, wherein the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid molecule at the active site. The amounts of each type of the nucleotide analogs in the extension solution after the extending step are then determined where each type is present in a second amount. The first and second amounts of each type of the nucleotide analog are compared. The type of nucleotide analog where the first and second amounts differ as the nucleotide added to the oligonucleotide primer at the active site is then identified. The steps of extending, determining the amounts of each type of the nucleotide analog, comparing the first and second amounts of the nucleotide analog, and said identifying the type of nucleotide analog added may be repeated, either after repeating the blending with the extended oligonucleotide primer or after determining the amounts of each type of dideoxynucleotide or dideoxynucleotide analog remaining in the extension solution as the new first amount. As a result, the nucleotide at the active site of the target nucleic acid molecule is determined. Also disclosed is an apparatus and composition for carrying out this method.

公开(公告)号：EP1248949A1

公开(公告)日：2002-10-16

申请号：EP01942714.5

申请日：2001-01-18

Applicant: Advion BioSciences, Inc.

Inventor： CORSO, Thomas, N. ,  SCHULTZ, Gary, A. ,  PROSSER, Simon, J. ,  HUANG, Xian

IPC: G01N27/26

CPC classification number: G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

Abstract: A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths (254) in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices (250) can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles (242) for the same fluid for analysis.

公开(公告)号：EP1163512A1

公开(公告)日：2001-12-19

申请号：EP00913655.7

申请日：2000-02-29

Applicant: Advion BioSciences, Inc.

Inventor： SCHULTZ, Gary, A. ,  CORSO, Thomas, N.

IPC: G01N30/72 ,  H01J49/10

CPC classification number: H01J49/167 ,  G01N30/6095 ,  G01N30/7266 ,  H01J49/0018

Abstract: A droplet/electrospray device and a liquid chromatography-electrospray system are disclosed. The droplet/electrospray device (100) comprises a substrate (102) defining a channel (104) between an entrance orifice (106) on an injection surface (108) and an exit orifice on an ejection surface (112), a nozzle (110) defined by a portion recessed from the ejection surface (112) surrounding the exit orifice, and an electrode (122) for application of an electric potential to the substrate (102) to optimize and generate droplets or an electrospray (62). A plurality of these electrospray devices (100) can be used in the form of an array of miniaturized nozzles. The liquid chromatography-electrospray device (160) comprises a separation substrate (162) defining an introduction channel (164) between an entrance orifice and a reservoir (166) and a separation channel (168) between the reservoir (166) and an exit orifice (170), the separation channel (168) being populated with separation posts (174) perpendicular to the fluid flow.