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    SINGLE-CELL NUCLEIC ACIDS FOR HIGH-THROUGHPUT STUDIES
    41.
    发明申请
    SINGLE-CELL NUCLEIC ACIDS FOR HIGH-THROUGHPUT STUDIES 审中-公开
    单细胞核酸用于高通量研究

    公开(公告)号：WO2016138490A1

    公开(公告)日：2016-09-01

    申请号：PCT/US2016/019952

    申请日：2016-02-26

    Applicant: FLUIDIGM CORPORATION

    Inventor： CONANT, Carolyn, G. ,  CHARN, Tze, Howe ,  WEST, Jason, A., A. ,  WANG, Xiaohui

    IPC: C40B20/00 ,  C40B30/04 ,  C40B40/06 ,  C40B50/06 ,  C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6874 ,  B01L3/5027 ,  C12Q1/6844 ,  C12Q2525/179 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

    Abstract translation: 本文描述的是基于细胞的分析方法，包括将核酸序列并入来自细胞群体的反应产物中的方法，其中所述核酸序列单独或以小组细胞单独掺入每个细胞的反应产物中。 本文还描述了允许至少两种试剂分别递送至每个细胞或细胞组的基质型微流体装置，以及在该方法和装置中有用的引物组合。

    INTEGRATED SINGLE CELL SEQUENCING
    42.
    发明申请
    INTEGRATED SINGLE CELL SEQUENCING 审中-公开
    集成单个细胞序列

    公开(公告)号：WO2015172080A1

    公开(公告)日：2015-11-12

    申请号：PCT/US2015/029986

    申请日：2015-05-08

    Applicant: FLUIDIGM CORPORATION

    Inventor： WEST, Jason A. A. ,  FOWLER, Brian ,  CHARN, Tze-Howe ,  JOHNSON, Christian F. ,  UNGER, Marc A.

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2565/501 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2537/162 ,  C12Q2565/543 ,  C12Q2535/122

    Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.

    Abstract translation: 本公开提供了形成标记的核酸序列的方法。 靶多核苷酸固定在固体支持物上; 识别寡核苷酸与其杂交; 识别寡核苷酸靶多核苷酸杂交体被切割; 并将衔接子核酸连接到切割的靶多核苷酸上，从而形成标记的核酸序列。 还提供了形成标记的单链cDNA的方法; 形成多个标记的异源核酸序列的方法; 识别寡核苷酸文库; 以及用于扩增固定在固体支持物上的cDNA序列的方法。 这些方法和产品可以单独使用或组合使用以用于集成的单细胞测序，并且可以适用于微流体装置或装置。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    44.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 审中-公开
    颗粒群体的单粒子分析

    公开(公告)号：WO2013177206A2

    公开(公告)日：2013-11-28

    申请号：PCT/US2013/042086

    申请日：2013-05-21

    Applicant: FLUIDIGM CORPORATION ,  ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，“并行地”执行一个或多个参数测量，即基本上同时在单独的反应体积中进行。

    MICROFLUIDIC DEVICES WITH REMOVABLE COVER AND METHODS OF FABRICATION AND APPLICATION
    47.
    发明申请
    MICROFLUIDIC DEVICES WITH REMOVABLE COVER AND METHODS OF FABRICATION AND APPLICATION 审中-公开
    具有可拆卸盖的微流体装置以及制造和应用方法

    公开(公告)号：WO2011040884A2

    公开(公告)日：2011-04-07

    申请号：PCT/SG2010/000369

    申请日：2010-09-29

    Applicant: FLUIDIGM CORPORATION ,  COHEN, David ,  MAY, Andrew ,  PIEPRZYK, Martin ,  FOWLER, Brian ,  LEE, Kim Huat ,  YAN, Jun ,  ZHOU, Ming Fang ,  NG, Seng Beng

    Inventor： COHEN, David ,  MAY, Andrew ,  PIEPRZYK, Martin ,  FOWLER, Brian ,  LEE, Kim Huat ,  YAN, Jun ,  ZHOU, Ming Fang ,  NG, Seng Beng

    IPC: G01N35/08 ,  G01N33/48

    CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

    Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

    Abstract translation: 本发明包括具有微型空腔的​​微流体系统，其可以被可移除的盖覆盖，其中可移除的盖允许微加工腔的至少一部分开口被操作者暴露或直接访问。 微流体系统包括在可包括PDMS的弹性体层中形成的腔室，流动和控制通道。 可移除的盖包括通过粘合剂层结合到弹性体层的热塑性基底膜。 当可移除的盖被剥离时，室至少部分地打开以允许从室中取样。 该室可能在PCR反应内部形成大分子晶体或产生内含物。 本发明还包括通过使用可移除盖制造弹性体层中的通孔的方法。 本发明还包括用于剥离可剥离盖或诸如集成式加热器撒布机的可移除部件的方法和装置。

    SELECTIVE TAGGING OF SHORT NUCLEIC ACID FRAGMENTS AND SELECTIVE PROTECTION OF TARGET SEQUENCES FROM DEGRADATION
    48.
    发明申请
    SELECTIVE TAGGING OF SHORT NUCLEIC ACID FRAGMENTS AND SELECTIVE PROTECTION OF TARGET SEQUENCES FROM DEGRADATION 审中-公开
    选择性标记短核酸片段和选择性保护目标序列从降解

    公开(公告)号：WO2010115044A2

    公开(公告)日：2010-10-07

    申请号：PCT/US2010/029690

    申请日：2010-04-01

    Applicant: FLUIDIGM CORPORATION ,  ZIMMERMANN, Bernhard G.

    Inventor： ZIMMERMANN, Bernhard G.

    IPC: C12Q1/68 ,  C12N15/10 ,  C12N15/11

    CPC classification number: C12N15/1093 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1072 ,  C12Q1/6846 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2537/159

    Abstract: Methods are provided for selective tagging of short nucleic acids comprising a short target nucleotide sequence over longer nucleic acids comprising the same target nucleotide sequence. The methods can involve performing one or two cycles of amplification of a sample comprising long nucleic acids and short nucleic acids, each comprising the same target nucleotide sequence with at least two target-specific primers or primer pairs under suitable annealing conditions, wherein the primer pairs comprise: an inner primer or primer pair that can amplify the target nucleotide sequence on long and short nucleic acids (wherein each inner primer comprises a 5' nucleotide tag; and an outer primer or primer pair that amplifies the target nucleotide sequence on long nucleic acids, but not on short nucleic acids); whereby the amplification after a second cycle produces at least one tagged target nucleotide sequence that comprises two nucleotide tags, one from each inner primer, with the target nucleotide sequence located between the nucleotide tags.

    Abstract translation: 提供了用于在包含相同靶核苷酸序列的较长核酸上选择性标记包含短目标核苷酸序列的短核酸的方法。 所述方法可以包括在合适的退火条件下进行包含长核酸和短核酸的样品的一个或两个循环的扩增，每个循环包含相同的靶核苷酸序列与至少两个靶特异性引物或引物对，其中引物对 包括：可以扩增长和短核酸上的靶核苷酸序列的内部引物或引物对（其中每个内引物包含5'核苷酸标签;以及扩增长核酸上的靶核苷酸序列的外引物或引物对 ，但不在短核酸上）; 由此在第二周期之后的扩增产生至少一个标记的靶核苷酸序列，其包含两个核苷酸标签，每个内引物中的一个，靶核苷酸序列位于核苷酸标签之间。

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