公开(公告)号：WO2016138490A1

公开(公告)日：2016-09-01

申请号：PCT/US2016/019952

申请日：2016-02-26

Applicant: FLUIDIGM CORPORATION

Inventor： CONANT, Carolyn, G. ,  CHARN, Tze, Howe ,  WEST, Jason, A., A. ,  WANG, Xiaohui

IPC: C40B20/00 ,  C40B30/04 ,  C40B40/06 ,  C40B50/06 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6874 ,  B01L3/5027 ,  C12Q1/6844 ,  C12Q2525/179 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

Abstract translation: 本文描述的是基于细胞的分析方法，包括将核酸序列并入来自细胞群体的反应产物中的方法，其中所述核酸序列单独或以小组细胞单独掺入每个细胞的反应产物中。 本文还描述了允许至少两种试剂分别递送至每个细胞或细胞组的基质型微流体装置，以及在该方法和装置中有用的引物组合。