Recombinational cloning using engineered recombination site
    41.
    发明专利
    Recombinational cloning using engineered recombination site 审中-公开
    使用工程改造现场的重组克隆

    公开(公告)号:JP2009100776A

    公开(公告)日:2009-05-14

    申请号:JP2009028314

    申请日:2009-02-10

    CPC classification number: C12N9/00 C12N15/10 C12N15/64 C12N15/66

    Abstract: PROBLEM TO BE SOLVED: To provide a DNA and vector having engineered recombination sites for use in recombinational cloning enabling an effective and specific recombination of a DNA segments using a recombination protein. SOLUTION: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s). COPYRIGHT: (C)2009,JPO&INPIT

    Abstract translation: 要解决的问题:提供具有工程重组位点的DNA和载体,用于重组克隆,使得能够使用重组蛋白进行DNA片段的有效和特异性重组。 解决方案:通过在体外和体内使用核酸,载体和方法提供重组克隆,用于使用工程重组位点和重组蛋白来移动或交换DNA分子片段以提供具有所需的嵌合DNA分子 特征和/或DNA片段。 版权所有(C)2009,JPO&INPIT

    大規模FETアレイを用いた分析物測定のための方法および装置

    公开(公告)号:JP2015092163A

    公开(公告)日:2015-05-14

    申请号:JP2014245830

    申请日:2014-12-04

    Abstract: 【課題】分析物測定のための大規模FETアレイの動作方法を提供する。【解決手段】化学感応性電界効果トランジスタ(chemFET)の大規模アレイは、アレイの個々のchemFETセンサ素子に近接して起こる分析物濃度の変化を検出するよう構成される。第1のタイミング信号に応答して、アレイの複数のセンサの信号サンプルを生成し、選択信号に応答して、複数のセンサに結合された出力回路を有効化し、出力回路に信号サンプルを出力する。一方、参照電圧を参照セルに印加し、参照セルから出力回路に参照信号を出力する。【選択図】なし

    音響収束ハードウェアおよび実装のためのシステムおよび方法
    44.
    发明专利
    音響収束ハードウェアおよび実装のためのシステムおよび方法 有权
    用于声音聚焦硬件和实现的系统和方法

    公开(公告)号:JP2014211454A

    公开(公告)日:2014-11-13

    申请号:JP2014166438

    申请日:2014-08-19

    Abstract: 【課題】音響収束ハードウェアおよび実装のためのシステムおよび方法の提供。【解決手段】音響収束毛細管は、溝を有する少なくとも1つの振動供給源に結合された毛細管を含む。音響収束毛細管を製造する方法は、毛細管と少なくとも1つの振動供給源とを提供することと、該振動供給源内に溝を機械加工することと、該溝において該少なくとも1つの振動供給源を該毛細管に連結することとを含む。別の方法は、粒子流を収束させることに関し、毛細管の外側領域の中へシース流体を流すことと、毛細管の中央コアの中へ、粒子流を流すことと、毛細管に沿った第1の位置において、粒子流に音響放射圧を印加することによって、粒子流を音響的に粒子を収束させることとを含む。粒子流は、第1の位置の下流の毛細管に沿った第2の位置において流体力学的に収束させることによってさらに収束され得る。【選択図】なし

    Abstract translation: 要解决的问题:提供用于声学聚焦硬件和实现的系统和方法。解决方案:声学聚焦毛细管包括耦合到至少一个具有凹槽的振动源的毛细管。 一种用于制造声聚焦毛细管的方法,包括以下步骤:提供毛细管和至少一个振动供应源; 进行振动源的槽的机械加工; 以及将所述至少一个振动供应源连接到所述凹槽中的毛细管。 另一方法包括以下步骤:将护套流体浇注到毛细管的外部区域; 将颗粒流入毛细管的中心芯; 以及通过在沿着毛细管的第一位置处对颗粒流的聚焦施加声辐射压力来声音聚焦颗粒流的颗粒。 通过在第一位置下游沿着毛细管的第二位置进行流体动力聚焦,可以进一步聚焦颗粒流。

    Dry powder cell, cell-culturing reagent, and method for producing them
    45.
    发明专利
    Dry powder cell, cell-culturing reagent, and method for producing them 有权
    干粉细胞,细胞培养试剂及其生产方法

    公开(公告)号:JP2014054267A

    公开(公告)日:2014-03-27

    申请号:JP2013266253

    申请日:2013-12-25

    CPC classification number: C12N5/0018 C12N1/14 C12N1/16 C12N1/20 C12N5/0025

    Abstract: PROBLEM TO BE SOLVED: To provide a nutrient medium and a medium supplement which are suitable for culturing bacteria, yeasts, and animal and plant cells, and to provide a method for producing the nutrient medium and the medium supplement.SOLUTION: There is provided a powder nutrient medium, particularly a cell culture medium supplement containing powdered serum, and a method for producing the medium and the medium supplement. Further, there is provided a kit and a method for culturing prokaryotic cells and eukaryotic cells by using the dry powder nutrient medium and the medium supplement, a sterilization method by γ-ray irradiation, and a method for producing a medium including a process of spraying and drying cell suspension. Furthermore, there is provided a method for producing a sterilized powder medium, a medium supplement (powdered serum, powdered transferrin, powdered insulin, powdered growth factor, etc.), a medium sub-group, and a buffer agent formulation, and a produced cell, the produced medium, the produced medium supplement, the produced medium sub-group, and the produced buffer agent powder.

    Abstract translation: 要解决的问题:提供适用于培养细菌,酵母和动植物细胞的营养培养基和培养基补充品,并提供营养培养基和培养基补充物的制备方法。解决方案:提供一种 粉末营养培养基,特别是含有粉末状血清的细胞培养基补充剂,以及生产培养基和培养基补充剂的方法。 此外,提供了通过使用干粉营养培养基和培养基补充剂,通过γ射线照射的灭菌方法培养原核生物细胞和真核细胞的试剂盒和方法,以及包括喷雾方法的培养基的制备方法 和干燥细胞悬液。 此外,提供了制造无菌粉末培养基,培养基补充剂(粉末状血清,粉状转铁蛋白,胰岛素粉末,粉末生长因子等),中等亚组和缓冲剂制剂的方法, 细胞,产生的培养基,产生的培养基补充物,产生的培养基亚组和产生的缓冲剂粉末。

    Pipette tip for electroporation
    46.
    发明专利
    Pipette tip for electroporation 审中-公开
    电源提示

    公开(公告)号:JP2014027952A

    公开(公告)日:2014-02-13

    申请号:JP2013232020

    申请日:2013-11-08

    CPC classification number: C12M35/02

    Abstract: PROBLEM TO BE SOLVED: To provide a pipette tip for electroporation.SOLUTION: Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.

    Abstract translation: 要解决的问题:提供用于电穿孔的移液管尖端。解决方案:本文提供了用于电穿孔的移液管末端,其包括外表面,位于外表面内的空隙和至少部分地位于外表面上或外表面上的导体, 与外表面和空隙的至少一部分电连通。 本文进一步提供用于电穿孔的移液管尖端,包括主体,连接器和细长部分。 连接器位于主体的远端,并且还包括至少一个连接柱,与连接器机械连通的连接部分和至少部分地位于连接器上或内部的导体,其中导体至少围绕 连接柱的一部分。 细长部分还具有空隙并且位于连接器的远端。 主体,连接器和细长部件的空隙处于流体连通状态。

    Dry powder cell and cell culture reagent and method for producing the same
    47.
    发明专利
    Dry powder cell and cell culture reagent and method for producing the same 审中-公开
    干粉细胞和细胞培养物试剂及其生产方法

    公开(公告)号:JP2013153759A

    公开(公告)日:2013-08-15

    申请号:JP2013081743

    申请日:2013-04-10

    CPC classification number: C12N5/0018 C12N1/00 C12N2500/36 C12N2500/60

    Abstract: PROBLEM TO BE SOLVED: To provide, specifically, a powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells, relating generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations.SOLUTION: There is provided such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. There are also provided methods for producing these formulations, and also kits and methods for cultivating prokaryotic and eukaryotic cells using these formulations, methods for producing sterile formulations, and methods for producing dry cell powders. There are also provided cells, media, media supplement, media subgroup, and buffer powders produced by the methods.

    Abstract translation: 要解决的问题:具体地,提供粉状营养培养基,培养基补充和中等亚组分制剂,特别是细胞培养基补充剂(包括粉末血清如粉末胎牛血清(FBS)),培养基亚组制剂和细胞培养基,其包含 促进体外培养细胞的必要营养因子,一般涉及营养(例如,细胞培养)培养基,培养基补充剂,培养基亚组和缓冲制剂。解决方案:提供这样的粉末制剂,其产生特定或期望的最终离子和/ 或用溶剂重构后的pH条件。 还提供了用于制备这些制剂的方法,以及使用这些制剂培养原核和真核细胞的试剂盒和方法,用于生产无菌制剂的方法和用于生产干细胞粉末的方法。 还提供了通过这些方法生产的细胞,培养基,培养基,培养基和缓冲粉。

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