Abstract:
PROBLEM TO BE SOLVED: To provide a DNA and vector having engineered recombination sites for use in recombinational cloning enabling an effective and specific recombination of a DNA segments using a recombination protein. SOLUTION: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s). COPYRIGHT: (C)2009,JPO&INPIT
Abstract:
PROBLEM TO BE SOLVED: To provide a nutrient medium and a medium supplement which are suitable for culturing bacteria, yeasts, and animal and plant cells, and to provide a method for producing the nutrient medium and the medium supplement.SOLUTION: There is provided a powder nutrient medium, particularly a cell culture medium supplement containing powdered serum, and a method for producing the medium and the medium supplement. Further, there is provided a kit and a method for culturing prokaryotic cells and eukaryotic cells by using the dry powder nutrient medium and the medium supplement, a sterilization method by γ-ray irradiation, and a method for producing a medium including a process of spraying and drying cell suspension. Furthermore, there is provided a method for producing a sterilized powder medium, a medium supplement (powdered serum, powdered transferrin, powdered insulin, powdered growth factor, etc.), a medium sub-group, and a buffer agent formulation, and a produced cell, the produced medium, the produced medium supplement, the produced medium sub-group, and the produced buffer agent powder.
Abstract:
PROBLEM TO BE SOLVED: To provide a pipette tip for electroporation.SOLUTION: Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.
Abstract:
PROBLEM TO BE SOLVED: To provide, specifically, a powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells, relating generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations.SOLUTION: There is provided such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. There are also provided methods for producing these formulations, and also kits and methods for cultivating prokaryotic and eukaryotic cells using these formulations, methods for producing sterile formulations, and methods for producing dry cell powders. There are also provided cells, media, media supplement, media subgroup, and buffer powders produced by the methods.
Abstract:
PROBLEM TO BE SOLVED: To provide methods and compositions for labeling nucleic acids.SOLUTION: The invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms.
Abstract:
PROBLEM TO BE SOLVED: To provide a method for preparing, isolating and expanding antigen-specific T cells. SOLUTION: The method for expanding cell population containing antigen-specific T cells with surfaces existing in 1:2 or less ratio of the surfaces over T cells includes bringing a first medicine to bind CD3/TCR complex on the T cells through a ligand, a second medicine to bind accessory molecules on the T cells through a ligand, and ligand bonds of the T cells with the first and second medicines to induce proliferation of antigen-specific T cells, in the cell population containing the antigen-specific T cells. COPYRIGHT: (C)2011,JPO&INPIT
Abstract:
PROBLEM TO BE SOLVED: To provide an ultra-sensitivity signal generation and detection system for multiplexed assays of analytes, permitting simple and efficient detection by using resonance light scattering (RLS) particles, and a signal generation and detection apparatus, facilitating the measurement and analysis of biological interactions on a variety of solid phase formats, including glass, plastic and membrane substrates and microwell plates. SOLUTION: The signal generation and detection system includes a control and analysis system 20, the signal generation and detection apparatus 100 or a reader for capturing, processing and analyzing the images of samples having resonance light scattering particle labels. COPYRIGHT: (C)2009,JPO&INPIT