公开(公告)号：EP3203852A2

公开(公告)日：2017-08-16

申请号：EP15787446.2

申请日：2015-10-06

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog ,  HANSEN, Marie Bendix

IPC: A23C9/146 ,  A23J1/20 ,  B01D15/38 ,  C07K16/04 ,  C07K1/16

CPC classification number: A23J1/20 ,  A23C9/1465 ,  A23C19/02 ,  A61K8/64 ,  A61K8/986 ,  A61K2800/10 ,  A61Q19/00 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/165 ,  C07K14/47

Abstract: The present invention relates to a method for separating at least one soluble protein fraction from an aggregated casein-containing material, the method comprises the steps of: (i) providing the aggregated casein-containing material; (ii) Contacting the aggregated casein-containing material with a chromatographic support allowing one or more soluble protein(s) present in the aggregated casein-containing material to be retained by the chromatographic support; (iii) Obtaining a permeate fraction from the chromatographic support comprising aggregated casein; (iv) Optionally washing the chromatographic support; (v) Subjecting the chromatographic support to at least one elution buffer obtaining at least one soluble protein fraction from the chromatographic support; and wherein the chromatographic support comprises one or more mixed-mode ligands capable of binding the soluble proteins from the aggregated casein-containing material.

Abstract translation: 本发明涉及从聚集的含酪蛋白的材料中分离至少一种可溶性蛋白质部分的方法，所述方法包括以下步骤：（i）提供聚集的含酪蛋白的材料; （ii）将聚集的含酪蛋白的材料与色谱载体接触，使得存在于聚集的含酪蛋白的材料中的一种或多种可溶蛋白被色谱载体保留; （iii）从包含聚集的酪蛋白的层析载体获得渗透物部分; （iv）任选洗涤色谱载体; （v）使色谱载体经历至少一种洗脱缓冲液，从色谱载体获得至少一种可溶性蛋白质部分; 并且其中所述色谱载体包含能够从所述聚集的含酪蛋白的材料结合所述可溶性蛋白的一种或多种混合模式配体。

公开(公告)号：EP3179862A1

公开(公告)日：2017-06-21

申请号：EP15753610.3

申请日：2015-08-14

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan Otto Fog. ,  HANSEN, Marie Bendix

IPC: A23C9/146 ,  A23J1/20 ,  C07K14/47

CPC classification number: A23J1/205 ,  A23C9/1465 ,  C07K1/18 ,  C07K14/4717 ,  C07K14/76

Abstract: The present invention relates to a method for providing an alpha-lactalbumin fraction and a beta-lactoglobulin fraction from a whey material obtained from milk, the method comprising the steps of: (i) providing the whey material; (ii) contacting the whey material with a chromatographic support allowing beta-lactoglobulin to be retained by the chromatographic support; (iii) obtaining a permeate fraction from the chromatographic support comprising the alpha-lactalbumin fraction; (iv) optionally washing the chromatographic support; and (v) obtaining a retentate fraction from the chromatographic support comprising the beta-lactoglobulin fraction; wherein the whey material provided in step (i) has been depleted, or substantially depleted from at least one whey protein, such as at least 2 whey proteins, e.g. at least 3 whey proteins.

Abstract translation: 本发明涉及从由乳获得的乳清材料提供α-乳清蛋白部分和β-乳球蛋白部分的方法，所述方法包括以下步骤：（i）提供乳清材料; （ii）使乳清材料与色谱载体接触，使得β-乳球蛋白被色谱载体保留; （iii）从包含α-乳清蛋白部分的色谱载体中获得渗透物部分; （iv）任选洗涤色谱载体; 和（v）从包含β-乳球蛋白部分的色谱载体中获得保留物部分; 其中步骤（i）中提供的乳清物质已经从至少一种乳清蛋白，例如至少2种乳清蛋白，例如乳清蛋白中消耗或基本耗尽。 至少3种乳清蛋白。

公开(公告)号：EP1552752B1

公开(公告)日：2011-07-13

申请号：EP05075201.3

申请日：2002-05-31

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： Andersen, Inga Vaarts ,  Hansen, Marie Bendix ,  Lihme, Allan Otto Fog ,  Olander, Morten Aae

IPC: A23J1/00 ,  A23J1/20 ,  C07K1/22

CPC classification number: B01D15/1807 ,  A23J1/00 ,  A23J1/20 ,  A23J1/205 ,  A23V2002/00 ,  B01D15/1871 ,  B01D15/265 ,  B01D15/424 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/286 ,  B01J20/3268 ,  B01J20/3293 ,  A23V2250/54242 ,  A23V2250/54244 ,  A23V2250/54248 ,  A23V2250/5425 ,  A23V2250/5434 ,  A23V2300/30

公开(公告)号：EP2277912A2

公开(公告)日：2011-01-26

申请号：EP10184552.7

申请日：2005-06-07

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： Lihme, Allan Otto Fog

IPC: C07K14/81 ,  C07K14/76 ,  C07K14/75 ,  C07K14/755 ,  C07K14/47

CPC classification number: B01D15/08 ,  B01D15/206 ,  B01D15/424 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/79 ,  C07K14/81 ,  C07K16/00

Abstract: The present invention provides a process for the isolation of one or more proteins from a protein solution. The process comprises the steps of: (a) providing a protein solution comprising one or more specific proteins and having a preset pH and a preset ionic strength or conductivity, (b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and (c) obtaining one or more proteins from the column, wherein the protein solution has been supplemented with an alcohol.

Abstract translation: 本发明提供了从蛋白质溶液中分离一种或多种蛋白质的方法。 该方法包括以下步骤：（a）提供包含一种或多种特定蛋白质并具有预设pH和预设离子强度或导电性的蛋白质溶液，（b）将蛋白质溶液施用于填充床或膨胀床柱，其包含 吸附剂，和（c）从所述塔中获得一种或多种蛋白质，其中所述蛋白质溶液已经用醇补充。

公开(公告)号：EP1567195B1

公开(公告)日：2010-10-27

申请号：EP03775106.2

申请日：2003-11-26

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： HEEGAARD, Peter ,  BOAS, Ulrik

IPC: A61K47/48 ,  C08G83/00

CPC classification number: B82Y5/00 ,  A61K47/6949 ,  C08G83/003

Abstract: Dendrimer conjugates are presented, which are formed between a dendrimer and a protein solubilising substance. Such dendrimer conjugates are effective in the treatment of protein aggregate-related diseases (e.g. prion-related diseases). The protein solubilising substance and the dendrimer together show a protein aggregate solubilising effect higher than a physical mixture of the dendrimer and the protein solubilising substance (i.e. a synergistic effect). Such dendrimer conjugates are useful in the treatment or prevention of protein aggregate-relates diseases, in disinfection/decontamination processes and in classifying or identifying protein aggregates. The synthesis of such dendrimer conjugates from readily-available starting materials is described.

公开(公告)号：EP2139573A1

公开(公告)日：2010-01-06

申请号：EP08735566.5

申请日：2008-03-28

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan ,  HOLTE, René, Oehlenschläger ,  JENSEN, Kurt, Hauge ,  CHRISTENSEN, Tony

IPC: B01D15/18 ,  G01N30/38

CPC classification number: B01D15/1807 ,  C07K1/22 ,  G01N30/38

Abstract: In the field of expanded bed adsorption chromatography, with particular but not exclusive relevance to disposable expanded bed chromatography columns, a method of conducting upward flow expanded bed chromatography comprising: supplying a liquid via an inlet to a stationary phase medium contained in a column, allowing adsorption of at least one component from the liquid by the stationary phase medium, withdrawing the liquid from the column via an outlet, regulating the expansion of the stationary phase medium by regulation of the flow rate of the liquid through at least the inlet, and restricting any overpressure in the headspace of the column to not more than the outside pressure plus 0.1 bar, is provided. In addition, apparatus for use in said method, in particular columns for use in expanded bed chromatography, are provided.

公开(公告)号：EP2112949A1

公开(公告)日：2009-11-04

申请号：EP08700941.1

申请日：2008-01-15

Applicant: UPFRONT CHROMATOGRAPHY A/S ,  Lihme, Allan Otto Fog

Inventor： LIHME, Allan Otto Fog

IPC: B01D15/02 ,  C12P7/10 ,  C07K1/22 ,  A23J1/00 ,  B01D15/00 ,  C10L1/02 ,  C10L1/18

CPC classification number: C10L1/1802 ,  B01D15/1807 ,  C07K1/16 ,  C12P3/00 ,  C12P5/023 ,  C12P7/02 ,  C12P7/08 ,  C12P7/16 ,  C12P7/62 ,  C12P7/649 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspensionfrom step (iii) to an expanded bed adsorption process obtaining the purified protein product.

公开(公告)号：EP1605772B1

公开(公告)日：2007-06-27

申请号：EP04721828.4

申请日：2004-03-19

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： HANSEN, Marie, Bendix ,  LIHME, Allan, Otto, Fog

IPC: A23J1/20 ,  A23J1/08 ,  B01D15/08

CPC classification number: C07K1/16 ,  A23J1/005 ,  A23J1/08 ,  A23J1/20 ,  A23J1/205 ,  B01D15/12 ,  B01D15/18 ,  B01D15/1807

Abstract: The present invention provides industrial scale expanded bed adsorption process for fractionation and isolation of bio-molecules from fluids, preferably proteins from milk and whey, in a cost-effective manner. This is accomplished by operating the expanded bed column at high temperatures of at last 40°C, combined with applying flow rates greater than 1.500 cm/hour.

公开(公告)号：EP1401289B1

公开(公告)日：2005-03-09

申请号：EP02745177.2

申请日：2002-05-31

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan, Otto, Fog ,  HANSEN, Marie, Bendix ,  ANDERSEN, Inga, Vaarst ,  OLANDER, Morten, Aae

IPC: A23J1/20 ,  A23J1/00 ,  A23J1/04 ,  A23J1/12 ,  A23J1/14 ,  C07K1/22 ,  B01D15/00

CPC classification number: B01D15/1807 ,  A23J1/00 ,  A23J1/20 ,  A23J1/205 ,  A23V2002/00 ,  B01D15/1871 ,  B01D15/265 ,  B01D15/424 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/286 ,  B01J20/3268 ,  B01J20/3293 ,  A23V2250/54242 ,  A23V2250/54244 ,  A23V2250/54248 ,  A23V2250/5425 ,  A23V2250/5434 ,  A23V2300/30

公开(公告)号：EP1401289A1

公开(公告)日：2004-03-31

申请号：EP02745177.2

申请日：2002-05-31

Applicant: UPFRONT CHROMATOGRAPHY A/S

Inventor： LIHME, Allan, Otto, Fog ,  HANSEN, Marie, Bendix ,  ANDERSEN, Inga, Vaarst ,  OLANDER, Morten, Aae

IPC: A23J1/20 ,  A23J1/00 ,  A23J1/04 ,  A23J1/12 ,  A23J1/14 ,  C07K1/22 ,  B01D15/00

CPC classification number: B01D15/1807 ,  A23J1/00 ,  A23J1/20 ,  A23J1/205 ,  A23V2002/00 ,  B01D15/1871 ,  B01D15/265 ,  B01D15/424 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/286 ,  B01J20/3268 ,  B01J20/3293 ,  A23V2250/54242 ,  A23V2250/54244 ,  A23V2250/54248 ,  A23V2250/5425 ,  A23V2250/5434 ,  A23V2300/30

Abstract: Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, said method comprising the steps of: a) optionally adjusting the pH of the mixture, b) applying said mixture to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 νm, c) optionally washing the column, d) eluting at least one protein from the adsorbent.