公开(公告)号：WO2017070154A4

公开(公告)日：2017-04-27

申请号：PCT/US2016/057612

申请日：2016-10-19

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： TARAFDER, Abhijit

IPC: G01N30/16 ,  G01N30/20 ,  G01N30/22 ,  G01N30/38

Abstract: A significant reduction in extra-column band broadening can be achieved by decoupling the injection system from the main solvent flow line. Systems and methods for such decoupling can allow for the injection of larger volumes of sample without compromising separation yield, increase the column loading per batch, and increase the overall yield of separations. For example, a mixture of co-solvent and sample can be prepared separately from the main flow of mobile phase and co-solvent (e.g., a mixture of CO 2 and methanol), loaded onto an injection loop, and then injected directly into the main flow of mobile phase and co-solvent before the chromatography column.

公开(公告)号：WO2017044530A1

公开(公告)日：2017-03-16

申请号：PCT/US2016/050628

申请日：2016-09-08

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： BIRDSALL, Robert

IPC: A61K39/395 ,  A61K47/48 ,  G01N33/53 ,  C12P21/08 ,  C07K1/20 ,  C07K1/22 ,  C07K1/14 ,  C07K1/16

CPC classification number: A61K39/395 ,  G01N33/53 ,  G01N33/94

Abstract: The present disclosure relates to a sensitive, multidimensional chromatography method for extraction, detection, and quantification of non-conjugated cytotoxic agents and associated linker molecules used in cysteine based antibody-drug-conjugate production.

Abstract translation: 本公开涉及用于提取，检测和定量非共轭细胞毒性剂和用于半胱氨酸基抗体 - 药物 - 缀合物生产中的相关连接分子的敏感的多维色谱方法。

公开(公告)号：WO2016196179A1

公开(公告)日：2016-12-08

申请号：PCT/US2016/034288

申请日：2016-05-26

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： ASTARITA, Giuseppe ,  MASON, Donald ,  BALOGH, Michael

IPC: G01N33/92 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50

CPC classification number: G01N33/03 ,  G01N27/62 ,  G01N33/92

Abstract: The present disclosure relates generally to methods and apparatus for determining essential nutrients and the ratio of essential nutrients in a sample. In particular, the present disclosure relates to the use of surface desorption ionization-mass spectrometry methods and apparatus to assay essential nutrients and ratios thereof, e.g., omega-6/omega-3 ratios.

Abstract translation: 本公开一般涉及用于确定样品中必需营养物质和必需营养物质比例的方法和装置。 特别地，本公开内容涉及表面解吸电离 - 质谱法方法和装置用于测定必需营养物质及其比例，例如ω-6 /ω-3比例。

公开(公告)号：WO2016138208A1

公开(公告)日：2016-09-01

申请号：PCT/US2016/019471

申请日：2016-02-25

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： FOGWILL, Michael, O. ,  MICHIENZI, Joseph, D. ,  GILAR, Martin ,  TARAFDER, Abhijit

IPC: G01N30/00 ,  G01N30/02 ,  G01N30/26 ,  G01N30/30

CPC classification number: G01N30/30 ,  G01N30/34 ,  G01N2030/3015 ,  G01N2030/3076

Abstract: Methods for focusing analyte peaks in liquid chromatography using a spatial temperature gradient are provided. Also provided are methods for focusing analyte peaks and improving resolution using a trap column upstream of a separation column. Further, methods are provided in which the trap column placed upstream of the separation column is packed with a temperature- sensitive polymer/copolymer, and a spatial temperature gradient is applied along the trap column for obtaining improved retentivity by trap column stationary phase, and overall improved resolution of analyte peaks.

Abstract translation: 提供了使用空间温度梯度在液相色谱中聚焦分析物峰的方法。 还提供了用于聚焦分析物峰的方法，并使用分离塔上游的捕获柱来提高分辨率。 此外，提供了设置在分离塔上游的捕集塔填充温度敏感性聚合物/共聚物的方法，并且沿着捕集塔施加空间温度梯度以通过陷阱柱固定相获得改进的保持性，并且总体上 提高分析物峰的分辨率。

公开(公告)号：WO2016089515A1

公开(公告)日：2016-06-09

申请号：PCT/US2015/058571

申请日：2015-11-02

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： CORMIER, Sylvain

IPC: G01N30/20

CPC classification number: G01N30/20 ,  G01N2030/202 ,  G01N2030/207

Abstract: A rotary valve used in chromatography includes a stator with a plurality of stator ports arranged on the stator. The stator further includes a stator groove connected at one end to a first stator port and terminating between the first stator port and a second stator port adjacent to the first stator port. A rotor, rotatably fitted to the stator, has a plurality of arcuate channels arranged in an asymmetrical pattern on the rotor. Each rotor channel connects to one or more of the stator ports. Different connections of the rotor channels to the stator ports produce at least three different positions for the injection valve. The three different positions provide a complete chromatography sample injection sequence using only a single valve.

Abstract translation: 用于色谱的旋转阀包括具有布置在定子上的多个定子口的定子。 定子还包括定子槽，其一端连接到第一定子口并且终止在第一定子口与邻近第一定子口的第二定子口之间。 可旋转地装配到定子的转子具有在转子上以不对称图案布置的多个弧形通道。 每个转子通道连接到一个或多个定子口。 转子通道与定子端口的不同连接为喷射阀产生至少三个不同的位置。 三个不同的位置提供了仅使用单个阀的完整的色谱分析样品注射顺序。

公开(公告)号：WO2015191993A1

公开(公告)日：2015-12-17

申请号：PCT/US2015/035540

申请日：2015-06-12

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： GEROMANOS, Scott, J. ,  CIAVARINI, Steven, J.

IPC: H01J49/10 ,  H01J49/14 ,  G01N30/72

CPC classification number: H01J49/0036 ,  G01N30/8675

Abstract: A method of mass spectrometry comprises ionising a sample and obtaining mass spectral data relating to a plurality of ion detection events. The method then comprises applying match tolerances for mass to charge ratio (m/z), chromatographic retention time (t r ), and ion mobility drift time (t d ), to the ion detection events in order to determine possible charge state connections. The method also comprises constructing a tentative isotope chain and querying ion detection events for a match to the tentative isotope chain. Once a tentative isotope chain has been constructed, the method further comprises determining a corresponding theoretical molecular mass and a corresponding theoretical isotopic distribution, querying one or more lookup tables and returning one or more parameters (New X, New X', Δ New X') related to the fractional mass to charge ratio (f m/z ) and at least one of: ion mobility drift time (t d ); and nominal mass to charge ratio (N m/Z ), of the ion detection events, and using the one or more parameters (New X, New X', Δ New X') to determine a unique charge state of the ions.

Abstract translation: 质谱法包括使样品电离并获得与多个离子检测事件相关的质谱数据。 该方法然后包括将质量与电荷比（m / z），色谱保留时间（tr）和离子迁移率漂移时间（td）的匹配公差应用于离子检测事件，以便确定可能的电荷状态连接。 该方法还包括构建临时同位素链和查询离子检测事件以与暂定同位素链匹配。 一旦建立了临时同位素链，该方法还包括确定对应的理论分子质量和相应的理论同位素分布，查询一个或多个查找表并返回一个或多个参数（New X，New X'，ΔNew X' ）与分数质荷比（fm / z）和离子迁移率漂移时间（td）中的至少一个相关。 和离子检测事件的标称质荷比（Nm / Z），并使用一个或多个参数（New X，New X'，ΔNew X'）来确定离子的独特电荷状态。

公开(公告)号：WO2015160650A1

公开(公告)日：2015-10-22

申请号：PCT/US2015/025293

申请日：2015-04-10

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： SHREVE, Joshua A. ,  JOUDREY, Kurt D.

IPC: G01N30/30

CPC classification number: G01N30/30 ,  G01N1/44 ,  G01N2030/3007

Abstract: A passive pre-heater assembly includes a thermally conductive heat-spreading block and a plurality of passive pre-heaters in thermally conductive communication with the heat-spreading block. The plurality of the pre-heaters exchanges heat with the thermally conductive heat-spreading block. Each pre-heater includes a thermally conductive base in thermal communication with the heat-spreading block, and a plurality of thermally conductive fins is in thermal communication with the thermally conductive base. The plurality of fins of each pre-heater exchanges heat convectively with ambient air and conductively with the thermally conductive base of that pre-heater. A given one of the passive pre-heaters further comprises a tube in thermally conductive contact with the thermally conductive base of the given passive pre-heater. The thermally conductive heat-spreading block exchanges heat with a fluid passing through the tube of the given passive pre-heater.

Abstract translation: 无源预热器组件包括导热散热块和与热扩散块导热连通的多个无源预热器。 多个预热器与导热散热块交换热量。 每个预热器包括与热扩散块热连通的导热基座，并且多个导热翅片与导热基底热连通。 每个预热器的多个散热片与环境空气对流交换热量，并与该预热器的导热底座导电地交换。 被动预热器中的给定的一个还包括与给定的无源预热器的导热底座导热接触的管。 导热散热块与通过给定无源预热器的管的流体进行热交换。

公开(公告)号：WO2015160648A1

公开(公告)日：2015-10-22

申请号：PCT/US2015/025287

申请日：2015-04-10

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： SHREVE, Joshua A. ,  JOUDREY, Kurt D.

IPC: B01D53/02 ,  B01D59/33 ,  F24H3/08 ,  G01N30/02

CPC classification number: G01N30/30 ,  B01D15/161 ,  B01D53/025 ,  F24H3/062 ,  F24H3/081 ,  F24H9/0073 ,  F24H9/1863 ,  F24H9/2071 ,  F24H2240/08 ,  F24H2250/06 ,  G01N2030/3007

Abstract: A column-conditioning enclosure includes a column chamber adapted to hold one or more chromatography separation columns. A duct system provides an airflow path around the column chamber such that the one or more chromatography separation columns held within the column chamber are isolated from the airflow path. An air mover disposed in the airflow path generates a flow of air within the duct system. A heat exchanger system disposed in the airflow path near the air to exchange heat with the air as the air flows past the heat exchanger system. The air circulates through the duct system around the column chamber, convectively exchanging heat with the column chamber to produce a thermally conditioned environment for the one or more chromatography separation columns held within the column chamber.

Abstract translation: 柱调节外壳包括适于容纳一个或多个色谱分离柱的柱室。 管道系统提供围绕柱室的气流路径，使得保持在柱室内的一个或多个色谱分离柱与气流路径隔离。 设置在气流路径中的鼓风机在管道系统内产生空气流。 一种热交换器系统，其设置在靠近空气的气流中，随着空气流过热交换器系统而与空气交换热量。 空气在柱室周围循环通过管道系统，与塔室对流地进行热交换，以产生用于保持在塔室内的一个或多个色谱分离柱的热调节环境。

公开(公告)号：WO2015134317A1

公开(公告)日：2015-09-11

申请号：PCT/US2015/017972

申请日：2015-02-27

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： BROUSMICHE, Darryl, W. ,  FAIRCHILD, Jacob, N. ,  HILL, Jason, F. ,  ISAAC, Giorgis ,  MORRIS, Michael, F. ,  WYNDHAM, Kevin, D.

IPC: B01D15/08 ,  B01D15/26 ,  B01J20/32

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3234 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  B01J2220/54 ,  B01J2220/80 ,  C07F7/1836

Abstract: The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group.

Abstract translation: 本公开涉及将目的化合物，特别是目标的不饱和化合物与混合物分离的方法。 使用具有色谱固定相材料的色谱柱分离化合物，用于各种不同的含有第一取代基和第二取代基的不同色谱方式。 在色谱条件下，第一取代基使化合物保留时间变化最小化。 第二取代基通过引入一个或多个芳族，多芳族，杂环芳族或多杂环芳族烃基来色谱和选择性地保留化合物，每个基团任选被脂族基团取代。

公开(公告)号：WO2015023533A1

公开(公告)日：2015-02-19

申请号：PCT/US2014/050353

申请日：2014-08-08

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： SHREVE, Joshua, A.

IPC: B01D15/16

CPC classification number: B01D15/40 ,  B01D15/16 ,  B01D15/163 ,  G01N30/32

Abstract: The present disclosure relates to an apparatus for regulating the average mobile phase density or pressure in a carbon dioxide based separation system. The apparatus includes a controller, a set of pressure or density sensors and a set of instructions capable of determining the pressure drop across a column and adjusting at least one system component or parameter to achieve a pre-determined average mobile phase density or pressure in the system.

Abstract translation: 本公开涉及一种用于调节基于二氧化碳的分离系统中的平均流动相密度或压力的装置。 该装置包括控制器，一组压力或密度传感器以及能够确定柱上的压降的一组指令，并且调节至少一个系统部件或参数，以实现预定的平均流动相密度或压力 系统。