Abstract:
본 발명은 2종 이상의 농작물 종자들에 대해 동시에 종자전염병 저항성 품종 여부를 대량으로 검정하는 방법을 제공한다. 본 발명에 따른 검정 방법은 (a) 처리액이 통과할 수 있는 제1 용기 2개 이상 또는 처리액이 통과할 수 있으면서 2개 이상의 구획을 갖는 제2 용기를 준비하는 단계; (b) 2종 이상의 농작물 종자들을 상기 용기에 치상하되, 서로 다른 종자들은 서로 다른 제1 용기 또는 제2 용기의 서로 다른 구획 내에 치상하는 단계; (c) 2종 이상의 농작물 종자들이 치상된 용기(들)을 종자전염병 병원균 현탁액이 수용되어 있는 욕조(bath)에 동시에 침지하여 접종하는 단계; 및 (d) 제1용기별 또는 제2용기의 각 구획별로 전단계에서 접종된 종자들을 육묘용 상자에 구별하여 파종하는 단계를 포함한다.
Abstract:
The present invention relates to a composition for selecting a rice breed for selecting a rice breed having leafhopper-resistance. The composition includes a primer which detects at least one oligonucleotide or a fraction thereof selected from a group consisting of RM18166, RM18171, and Indel15040 as DNA markers. More specifically, the composition includes one or more primer sets selected from a group consisting of SEQ ID: 1-6. In addition, the present invention relates to a method and a kit for selecting a rice breed having leafhopper-resistance. The method includes the steps of: separating genome DNAs from a rice sample; amplifying a target sequence by performing amplification reaction using one or more primer sets which uses the separated genome DNAs as a template and is selected from a group consisting of SEQ ID: 1-6; and detecting the amplified product. When the method of the present invention is used, rice individuals which have leafhopper-resistance and sensitivity can be efficiently identified by using the makers, which are very closely related to locations of leafhopper-resistant gene.
Abstract:
The present invention relates to a method for promoting the production of Monacolin K in red yeast rice, and a device for rotating and culturing the same. The method comprises a method for culturing red yeast rice which is contained in a culturing container. The culturing container is horizontally laid between two support rods which can be rotated in order to come in contact with the support rods. The culturing container is rotated at a predetermined speed using electric power of the support rods so that the culturing container which comes in contact with the surface of a roller is rotated together and the red yeast rice is cultured. When a large amount of red yeast rice is cultured using the device of the present invention, the production amount of Monacolin K can be more increased than when red yeast rice is cultured through existing culturing methods. Moreover, large-scale contamination which is generated when the red yeast rice is cultured in a rotating culturing container can be prevented and an automatic agitating device can be utilized so that uniform a solid fermented material can be obtained. The device can be mechanically operated so that a person does not have to perform agitation for culturing in bags by himself/herself and required man power can be reduced.