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公开(公告)号：KR101582202B1
公开(公告)日：2016-01-05
申请号：KR1020140152646
申请日：2014-11-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A61L27/3637 , A61F2/0063 , A61K9/70 , A61K35/63 , A61L27/3604 , A61L27/3691 , A61L27/44 , A61L27/507 , A61L27/56
Abstract: 본발명은누에고치를이용한혈관용패치및 이의제조방법에관한것이다. 본발명의누에고치를이용한혈관용패치는제 1두께의단면을갖는누에고치에서두 개이상의적절한형태로평면분할된상기누에고치편으로제조되는것이특징이다. 본발명에의해, 제조과정이상대적으로간단하여통상의혈관용패치의단가와대비하여훨씬절감이가능함과동시에세포배양능이우수하며생체적합성을갖는누에고치를이용한혈관용패치및 이의제조방법이제공된다.
Abstract translation: 本发明涉及使用茧的血管贴片及其制造方法。 使用茧的血管贴片由茧片制成，其中茧片是通过将具有第一厚度的横截面的茧分成基于横截面切片的两个或更多个适当形状的片而获得的。 提供使用茧的血管贴片及其制造方法，其能够通过相对简化的制造方法与常规血管贴片的单位成本相比显着降低成本，同时具有优异的细胞增殖能力和生物相容性。
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公开(公告)号：KR1020150136360A
公开(公告)日：2015-12-07
申请号：KR1020140063757
申请日：2014-05-27
Applicant: 강릉원주대학교산학협력단 , 대한민국(농촌진흥청장)
Abstract: 본발명은혈관의수복을위한혈관용패치에관한것으로, 구체적으로는손상된혈관의결손부에정밀하게봉합되어, 혈관벽이재생되는동안피가새지않게하며, 외부환경으로부터손상된혈관의재생이방해받지않도록보호하고, 혈행의감소및 혈관내경의감소를최소화하는동시에, 혈관결손부위로새로운혈관벽을재생시키기위한혈관용패치에관한것이다.
Abstract translation: 本发明涉及一种用于修复血管的血管补片，更具体地，涉及一种通过精确地附着在损伤的血管的受影响区域上而在血管壁再生时防止血液泄漏的血管补片。 本发明的血管贴片还保护受损的血管免受外部环境的影响，以防止再生中断。 此外，血管贴片最小化血液循环的减少和血管的内半径，同时诱导血管中受影响区域上的新血壁的再生。
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公开(公告)号：KR1020150084152A
公开(公告)日：2015-07-22
申请号：KR1020140003852
申请日：2014-01-13
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/62 , A01K67/027
CPC classification number: C12N15/63 , C12N15/64 , C12N15/65 , C12N15/85 , C12N15/8509
Abstract: 본발명은재조합항균펩타이드세크로핀 B1을생산하는형질전환누에, 및상기형질전환누에를이용하여재조합항균펩타이드세크로핀 B1을생산하는형질전환누에를대량생산하는방법에관한것으로, 보다상세하게는표지유전자조절프로모터, 표지유전자, 누에유래프로모터및 누에유래 BmCecB1 합성유전자가작동가능하게연결된유전자컨스트럭트를포함하는재조합발현벡터를제작하여상기재조합발현벡터로누에를형질전환시킴으로써항균펩타이드가함유된누에를생산하는형질전환누에를제조할수 있으며, 이로인해천연항생제로서개발이가능하여, 가축사료첨가제뿐만아니라화장품, 치약등 생활용품의소재등으로유용하게사용될수 있다.
Abstract translation: 本发明涉及生产重组抗菌肽天蚕素B1的转基因蚕和通过使用转基因蚕大量生产重组抗菌肽天蚕素B1的转基因蚕的方法，更具体地涉及可含有连接DNA构建体的重组表达载体的制备 将DNS控制启动子，标记DNA，家蚕促生物和BmCecB1合成DNA和转化蚕转化为重组表达载体，以产生能够产生含有抗细菌肽的蚕的转基因蚕，从而能够开发天然抗生素并用作原料 用于日常生活用品，如化妆品或牙膏以及动物饲料。
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公开(公告)号：KR1020150056915A
公开(公告)日：2015-05-28
申请号：KR1020130139545
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/86 , A01K67/033
CPC classification number: C12N15/85
Abstract: 본발명은서열번호 3으로표시되는누에형질전환용고발현유전자프로모터, 상기고발현유전자프로모터를포함하는누에형질전환용고발현벡터, 및상기고발현벡터로형질전환된누에형질전환체에관한것이다. 본발명에따른누에형질전환용프로모터및 이를포함하는고발현벡터는종래의 BmA3 프로모터보다견사선또는누에생체에서의고발현을유도할수 있어, 누에에서의이형단백질생산성향상과정제과정의편리성에의하여산업적으로매우유용하다.
Abstract translation: 本发明涉及用于转化如SEQ ID NO：3所示的蚕的高表达基因启动子，包含用于转化蚕的高表达载体和由其转化的转基因蚕。 根据本发明，高表达基因启动子和载体可以比常规BmA3启动子更有效地诱导蚕丝或丝腺的高表达，从而增加蚕的变异蛋白质的产生，并使工业上更容易的纯化过程 利用。
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45.发明授权
公开(公告)号：KR101359510B1
公开(公告)日：2014-02-12
申请号：KR1020110120127
申请日：2011-11-17
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 누에 혈림프 특이적 발현 유전자의 프로모터 및 이 유전자의 최대 프로모터 활성영역에 관한 것이다.
본 발명의 누에 혈림프 특이적 발현 프로모터는 누에로부터 분리하여 서열번호 1로 표현되는 것이 특징이다.
본 발명에 의해, 누에의 혈림프에 특이적으로 발현되어 형질전환 누에로부터 유용한 유전자 재조합 단백질을 생산하도록 한 누에 혈림프 특이적 발현 유전자의 프로모터 및 이 유전자의 최대 프로모터 활성영역이 제공된다.-
Patent Agency Ranking46.发明公开인간 ＧＭ－ＣＳＦ２ 재조합 단백질을 갖는 누에 형질전환체의 제조방법 및 이를 통해 생산된 누에 형질전환체, 형질전환누에 无效具有人造粒细胞巨噬细胞集落刺激因子的丝状体转化的制造方法2重组蛋白和丝变性由方法获得的丝状体
公开(公告)号：KR1020140012406A
公开(公告)日：2014-02-03
申请号：KR1020120079203
申请日：2012-07-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a method for producing a silkworm transformant having human GM-CSF2 recombinant protein, a silkworm transformant produced thereby, and a transformed silkworm. The method for producing a silkworm transformant having human GM-CSF2 recombinant protein according to the present invention comprises the steps of: introducing bFibH promoter and 3xP3 promoter into a transfer vector to prepare an expression vector for silkworm transformation; and micro-injecting the expression vector for silkworm transformation into a silkworm egg to prepare a transduced silkworm transformant. The present invention provides: a method for producing a silkworm transformant, capable of mass-producing human-derived GM-CSF2 recombinant protein useful to humans at low costs; a silkworm transformant produced thereby; and a transformed silkworm.
Abstract translation: 本发明涉及具有人GM-CSF2重组蛋白，由其制备的蚕转化体和转化的蚕的蚕转化体的制备方法。 本发明的具有人GM-CSF2重组蛋白的蚕转化体的制造方法包括将bFibH启动子和3xP3启动子引入转移载体中以制备蚕转化表达载体的步骤。 并将蚕转化的表达载体微注射到蚕卵中以制备转导的蚕转化体。 本发明提供：能够以低成本大规模生产对人类有用的人源GM-CSF2重组蛋白质的蚕转化体的制造方法。 由此产生的蚕转化体; 和转化的蚕。
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公开(公告)号：KR1020130141973A
公开(公告)日：2013-12-27
申请号：KR1020120065146
申请日：2012-06-18
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a food composition containing Dpp protein originated from silkworm as an active ingredient for the prevention and treatment of bone-related diseases. The present invention provides a food composition for the prevention and treatment of bone-related diseases containing Dpp protein originated from silkworm which contains the amino acid sequence with the sequence number 2 or a base sequence with the sequence number 2 for coding the amino acid sequence. The food composition for the prevention and treatment of bone-related diseases contains the Dpp protein originated from silkworm which is separated from silkworm and induces the production of bones by improving the differentiation of osteoblast necessary for the bone production.
Abstract translation: 本发明涉及含有源自蚕的Dpp蛋白作为预防和治疗骨相关疾病的有效成分的食品组合物。 本发明提供一种用于预防和治疗含有源自蚕的Dpp蛋白质的骨相关疾病的食物组合物，其含有序列号2的氨基酸序列或编码氨基酸序列的序列号为2的碱基序列。 用于预防和治疗骨相关疾病的食物组合物含有源自蚕的Dpp蛋白质，其通过改善骨产生所需的成骨细胞的分化来诱导骨骼的产生。
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公开(公告)号：KR1020110127324A
公开(公告)日：2011-11-25
申请号：KR1020100046741
申请日：2010-05-19
Applicant: 한림대학교 산학협력단 , 인하대학교 산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: A61F2/02 , A61L27/227 , C08J3/205 , C08L89/00
Abstract: PURPOSE: An artificial dura mater and a manufacturing method thereof are provided to guarantee safety in operation and to do not cause inflammation in the operation. CONSTITUTION: Silk fibroin aqueous solution is cast in smooth and flat place and is dried. The crystallization induction of the dried silk fibroin is executed. A crystallization induction step puts the dried silk fibroin is executed into an enclosure container in which relative humidity is over 80% and executes annealing with water.
Abstract translation: 目的：提供人造硬膜及其制造方法，以保证手术中的安全性，并且不会在手术中引起炎症。 构成：将丝素蛋白水溶液浇铸在光滑平坦的地方，并干燥。 干燥的丝素蛋白的结晶诱导被执行。 结晶诱导步骤将干燥的丝素蛋白进行到相对湿度超过80％的封闭容器中，并用水进行退火。
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49.发明公开누에 프로테인 디설피드 이소머레이즈 유전자를 이용한 재조합 인간 각질세포 성장인자의 제조방법 无效通过使用BOMBYX MORI蛋白质半胱氨酸异构酶基因生产重组人KERATINOCYTE生长因子的方法
公开(公告)号：KR1020110120662A
公开(公告)日：2011-11-04
申请号：KR1020100040172
申请日：2010-04-29
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for preparing recombinant human keratinocyte growth factor using Bombyx mori protein disulfide isomerase gene is provided to improve productivity. CONSTITUTION: A method for preparing recombinant human keratinocyte growth factor comprises: a step of transforming insect cell line with a recombinant expression vector containing the gene; a step of simultaneously or gradually transforming insect cell line with a recombinant vector containing human keratinocyte growth factor gene; a step of selecting transformed cell line which expresses Bombyx mori protein disulfide isomerase and human keratinocyte growth factor; and a step of producing the recombinant human keratinocyte growth factor.
Abstract translation: 目的：提供使用蚕豆蛋白二硫键异构酶基因制备重组人角质形成细胞生长因子的方法，以提高生产力。 构成：制备重组人角质形成细胞生长因子的方法包括：用含有该基因的重组表达载体转化昆虫细胞系的步骤; 用包含人角质形成细胞生长因子基因的重组载体同时或逐渐转化昆虫细胞系的步骤; 选择表达家蚕蛋白二硫键异构酶和人角质形成细胞生长因子的转化细胞系的步骤; 以及产生重组人角质形成细胞生长因子的步骤。
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公开(公告)号：KR101060910B1
公开(公告)日：2011-08-30
申请号：KR1020090040200
申请日：2009-05-08
Applicant: 한림대학교 산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: A61L27/3641 , A61F2/18 , A61F11/00 , A61F2002/183 , A61L27/3604 , A61L27/38 , A61L2430/14
Abstract: Provided are an artificial eardrum using silk protein and a method of fabricating the same. The artificial eardrum is fabricated in the form of a silk membrane by desalinating and drying silk protein (or silk fibroin) or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber. Thus, regeneration of an eardrum perforated due to disease or a sudden accident is stimulated, a boundary of the regenerated eardrum is clean and biocompatibility and transparency are increased. In addition, the artificial eardrum may be fabricated using the silk protein or silk protein complex solution obtained from a silkworm cocoon alone or mixed with collagen, alginic acid, PEG or pluronic 127.
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