公开(公告)号：KR1020090072684A

公开(公告)日：2009-07-02

申请号：KR1020070140876

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/31 ,  C07K14/315

Abstract: A promoter and entire genome sequence of heat shock protein isolated from a silk worm is provided to express a excess amount foreign gene and easily select transformant of silk worm. A gene sequence of heat shock protein 70 gene denoted by the sequence 1 comprises gene area and promoter area of heat shock protein 70 isolated silk worm. The Cdna sequence of heat shock protein 70 is denoted by the sequence 2. The amino acid sequence denoted by the sequenc 3 is deduced by cDNA sequence. The maximum promoter activation area of heat shock protein 70 gene comprises 367-1517 sequences.

Abstract translation: 提供从丝虫分离的热休克蛋白的启动子和全基因组序列，以表达过量的外源基因，并容易选择蚕丝的转化体。 由序列1表示的热休克蛋白70基因的基因序列包含热休克蛋白70分离的丝虫的基因面积和启动子面积。 热休克蛋白70的Cdna序列由序列2表示。由序列3表示的氨基酸序列由cDNA序列推导出。 热休克蛋白70基因的最大启动子活化区域包含367-1517个序列。

公开(公告)号：KR100918016B1

公开(公告)日：2009-09-24

申请号：KR1020070140875

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/12 ,  C12N15/10 ,  C12N15/09

Abstract: 본 발명은 누에로부터 분리한 연장인자-1 알파 유전자의 전체 게놈 유전자 서열 및 최대 프로모터 활성 부위에 관한 것이다.
누에, 프로모터, 형질전환운반체, 액틴3, 연장인자-1 알파, 루시퍼레이즈, piggyBac

公开(公告)号：KR1020110120662A

公开(公告)日：2011-11-04

申请号：KR1020100040172

申请日：2010-04-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김성렬 ,  강석우 ,  강민욱

IPC: C12N15/61 ,  C12N15/12 ,  C12N15/85 ,  C12P21/02

Abstract: PURPOSE: A method for preparing recombinant human keratinocyte growth factor using Bombyx mori protein disulfide isomerase gene is provided to improve productivity. CONSTITUTION: A method for preparing recombinant human keratinocyte growth factor comprises: a step of transforming insect cell line with a recombinant expression vector containing the gene; a step of simultaneously or gradually transforming insect cell line with a recombinant vector containing human keratinocyte growth factor gene; a step of selecting transformed cell line which expresses Bombyx mori protein disulfide isomerase and human keratinocyte growth factor; and a step of producing the recombinant human keratinocyte growth factor.

Abstract translation: 目的：提供使用蚕豆蛋白二硫键异构酶基因制备重组人角质形成细胞生长因子的方法，以提高生产力。 构成：制备重组人角质形成细胞生长因子的方法包括：用含有该基因的重组表达载体转化昆虫细胞系的步骤; 用包含人角质形成细胞生长因子基因的重组载体同时或逐渐转化昆虫细胞系的步骤; 选择表达家蚕蛋白二硫键异构酶和人角质形成细胞生长因子的转化细胞系的步骤; 以及产生重组人角质形成细胞生长因子的步骤。

公开(公告)号：KR1020090072683A

公开(公告)日：2009-07-02

申请号：KR1020070140875

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/12 ,  C12N15/10 ,  C12N15/09

Abstract: An elongation factor-1 alpha promoter of a silk worm is provided to increase the expression of foreign gene and massively produce recombinant protein. An elongation factor-1 alpha gene sequence of the sequence number 1 comprises gene area and promoter of the elongation factor-1 alpha gene. A cDNA sequence of elongation factor-1 alpha gene is denoted the sequence 2. The amino acid sequence denoted by the sequence number 3 is deduced. The maximum promoter activation area of elongation factor-1 alpha gene comprises 1-1542 sequences.

Abstract translation: 提供丝虫的延伸因子-1α启动子以增加外源基因的表达并大量生产重组蛋白。 序列号1的延伸因子-1α基因序列包含延伸因子-1α基因的基因区域和启动子。 延伸因子-1α基因的cDNA序列被表示为序列2.由序列号3表示的氨基酸序列被推导出。 延伸因子-1α基因的最大启动子活化区域包含1-1542个序列。