Abstract:
PURPOSE: A primer composition for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid, and a use thereof are provided to quickly diagnose using high concentration SYBR Green I with naked eye under natural light. CONSTITUTION: A primer set for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid contains sequence in sequence numbers 1-4. The Chrysanthemum chlorotic mottle viroid is SSHA6 strains or msim34 strains. A primer composition for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer.
Abstract translation:目的:用于环路介导的等温扩增用于检测菊花褪绿斑马疹的底漆组合物,并提供其用途,在自然光下用肉眼快速诊断高浓度SYBR Green I。 构成:用于检测菊花褪绿斑马疹的环介导等温扩增的引物组包含序列号1-4中的序列。 菊花褪绿斑马疹是SSHA6菌株或msim34菌株。 用于检测菊花褪绿斑驳病毒的环介导等温扩增的底漆组合物含有引物组。 该组合物还含有DNA聚合酶,dNTP和反应缓冲液。
Abstract:
PURPOSE: A primer set for detecting begomovirus is provided to accurately and quickly diagnose begomovirus infection from plant samples. CONSTITUTION: A primer set for detecting begomovirus comprises one or more kinds of primer sets selected among a primer set of: sequence numbers 1 and 2; sequence numbers 3 and 4; sequence numbers 5 and 6; sequence number 7 and 8; sequence numbers 9 and 10; sequence number 11 and 12; sequence numbers 13 and 14; sequence number 15 and 16; sequence numbers 17 and 18; and sequence numbers 19 and 20. The begomovirus includes tomato leaf curl virus, tomato yellow leaf curl virus, tobacco leaf curl virus, honeysuckle yellow vein virus, or sweet potato leaf curl virus.