公开(公告)号：KR1020150122542A

公开(公告)日：2015-11-02

申请号：KR1020140048950

申请日：2014-04-23

Applicant: 포항공과대학교 산학협력단

Inventor： 차형준 ,  임슬기 ,  조병훈

IPC: C12N15/60 ,  C12N9/88 ,  C12N15/70

Abstract: 본발명은이산화탄소의수화반응을촉진할수 있는, 해양박테리아유래,구체적으로하이드로게노비브리오마리누스() 유래의탄산무수화효소를발현시킨형질전환체, 이의상기형질전환세포의세포파쇄액또는이의분액또는상기형질전환세포에서분리한탄산무수화효소를포함하는온도와 pH에대한안정성이뛰어난생물촉매로서의이산화탄소포집용조성물, 이들의제조방법및 이들을이용한이산화탄소의포집방법에관한것이다. 본발명의해양박테리아유래의탄산무수화효소는다양한 pH 범위및 높은온도에서도탁월한활성을유지할수 있으므로, 실제이산화탄소포집공정에매우유용하게적용될수 있다. 또한대장균시스템을이용하여대량생산이가능하므로경제성측면에서이점을가지고있다.

Abstract translation: 本发明涉及一种用于收集作为海洋细菌的重组生物催化剂的CO 2的组合物，其能够促进CO 2的水合反应并且对于温度和pH具有极好的安全性。 具体地说，海洋细菌衍生的重组生物催化剂包括从转化细胞或分离液或转化细胞的细胞破碎液中分离的表达氢原虫素衍生的碳酸酐酶和碳酸酐酶的转化体和使用其的收集二氧化碳的方法。 本发明的海洋细菌衍生的碳酸酐酶可以在各种pH范围和高温下保持优异的活化，并且有效地应用于真正的CO 2收集过程。 此外，本发明在经济方面有益，因为用于收集CO 2的组合物可以通过使用大肠杆菌系统大量生产。

公开(公告)号：KR101412773B1

公开(公告)日：2014-06-30

申请号：KR1020110031824

申请日：2011-04-06

Applicant: 포항공과대학교 산학협력단

Inventor： 차형준 ,  최유성

IPC: C07K14/435 ,  C12N15/12 ,  C12N15/70 ,  C09J189/00

Abstract: 본 발명은 티로신 잔기가 도파 또는 도파 퀴논으로 변형된 재조합 홍합 접착 단백질 및 이의 제조방법에 관한 것이다. 보다 구체적으로, 본 발명은 티로신 잔기가 도파 또는 도파 퀴논으로 변형된 재조합 홍합 접착 단백질 및 이의 제조방법, 상기 재조합 홍합 접착 단백질을 제조하긴 위한 형질전환체, 및 상기 재조합 홍합 접착 단백질의 접착제로서의 용도에 관한 것이다.

公开(公告)号：KR1020140035251A

公开(公告)日：2014-03-21

申请号：KR1020130106599

申请日：2013-09-05

Applicant: 포항공과대학교 산학협력단

Inventor： 황동수 ,  차형준 ,  오동엽 ,  김상식 ,  김범진

IPC: D04H1/42 ,  D04H1/728 ,  A61L27/56 ,  A61L27/22

CPC classification number: D04H1/4266 ,  A61L27/22 ,  A61L27/26 ,  A61L27/3637 ,  A61L27/56 ,  D04H1/4282 ,  D04H1/728 ,  D10B2509/00

Abstract: The present invention relates to a nanofibers tissue engineering scaffold comprising mussels coating proteins and Fe3+ ions and a manufacturing method thereof. More specifically, the present invention relates to a nanofibers tissue engineering scaffold which the surface of the nanofibers is coated by bioactive substances, the mussels adhesive proteins is a nanofibers tissue engineering scaffold which more than 20% of the total tyrosine residues are modified to DOPA.

Abstract translation: 本发明涉及包含贻贝涂层蛋白质和Fe3 +离子的纳米纤维组织工程支架及其制造方法。 更具体地，本发明涉及纳米纤维表面被生物活性物质包被的纳米纤维组织工程支架，贻贝粘合蛋白是纳米纤维组织工程支架，其中20％以上的酪氨酸残基被修饰为DOPA。

公开(公告)号：KR1020140027031A

公开(公告)日：2014-03-06

申请号：KR1020130096424

申请日：2013-08-14

Applicant: 포항공과대학교 산학협력단

Inventor： 차형준 ,  김범진 ,  황동수 ,  오동엽 ,  송영훈

IPC: A61L27/52 ,  A61K9/06 ,  A61K38/18 ,  A61K47/42

CPC classification number: A61L27/52 ,  A61K9/06 ,  A61K38/18 ,  A61K47/42 ,  A61L2430/00

Abstract: The present invention relates to a mussel adhesive protein-based bioadhesive hydrogel and a manufacturing method thereof. More specifically, to a hydrogel formed by combination of a DOPA (3, 4-dihydroxyphenylalanine) residue included in a mussel adhesive protein and Fe3+ ions. In addition, the present invention relates to a hydrogel formed as a DOPA residue contained in a mussel adhesive protein is oxidized.

Abstract translation: 本发明涉及一种基于贻贝粘合蛋白的生物粘附性水凝胶及其制造方法。 更具体地说，涉及通过组合包含在贻贝粘合蛋白质中的DOPA（3,4-二羟基苯丙氨酸）残基和Fe 3+离子形成的水凝胶。 此外，本发明涉及由贻贝粘合蛋白中所含的DOPA残基形成的水凝胶被氧化。

公开(公告)号：KR1020130033996A

公开(公告)日：2013-04-04

申请号：KR1020120107890

申请日：2012-09-27

Applicant: 포항공과대학교 산학협력단

Inventor： 황동수 ,  오동엽 ,  김상식 ,  차형준

IPC: C08B37/08 ,  C08L5/08 ,  A61L27/20 ,  A61L33/08

CPC classification number: A61L27/20 ,  A61L27/50 ,  A61L2430/10 ,  A61L2430/12 ,  C08B37/003 ,  C08L5/08 ,  Y10T442/60

Abstract: PURPOSE: A chitosan and/or chitin composite is provided to improve strength in a wet condition and to provide an artificial tendon and ligament for being used in a wet condition. CONSTITUTION: A composite comprises chitosan, chitin, or a mixture thereof; and one or more compounds selected from catechol, dopamine, DOPA, and methylcatechol. The one or more compounds selected from catechol, dopamine, DOPA, and methylcatechol has a crosslinked structure by a covalent bond or non-covalent bond. The composite has a Young's modulus of 500-10,000MPa at relative humidity of 40-50% and a Young's modulus of 180-5,000MPa at relative humidity of 90-100%.

Abstract translation: 目的：提供壳聚糖和/或几丁质复合材料，以提高湿润条件下的强度，并提供用于湿润条件的人造腱和韧带。 构成：复合材料包含壳聚糖，几丁质或其混合物; 和一种或多种选自邻苯二酚，多巴胺，DOPA和甲基儿茶酚的化合物。 一种或多种选自儿茶酚，多巴胺，DOPA和甲基儿茶酚的化合物通过共价键或非共价键具有交联结构。 该复合材料的相对湿度为40-50％时的杨氏模量为500-10,000MPa，相对湿度为90-100％时的杨氏模量为180-5,000MPa。

公开(公告)号：KR1020130021951A

公开(公告)日：2013-03-06

申请号：KR1020110084543

申请日：2011-08-24

Applicant: 포항공과대학교 산학협력단

Inventor： 차형준 ,  김범진 ,  최유성 ,  최봉혁

IPC: A61L27/34 ,  A61L27/54 ,  A61L27/22 ,  A61L27/04

CPC classification number: C12N5/0068 ,  B82Y5/00 ,  C07K14/43504 ,  C12N5/0654 ,  C12N2533/10 ,  C12N2533/40 ,  C12N2533/50 ,  C12N2533/70 ,  C12N2533/80 ,  C12N2537/00

Abstract: PURPOSE: Surface immobilization of various functional biomolecules is provided to conveniently implement an artificial extracellular substrate. CONSTITUTION: Surface immobilization of various functional biomolecules is as follows. A tissue engineering supporter comprises mussel adhesive protein and bioactive substances adhered to the mussel adhesive protein. The mussel adhesive protein and bioactive substances are coated on the surface of metal. The mussel adhesive protein is protein consisting of amino acid sequence with sequence number 4.

Abstract translation: 目的：提供各种功能性生物分子的表面固定以方便地实施人造细胞外基质。 构成：各种功能性生物分子的表面固定如下。 组织工程支持物包括贻贝粘合蛋白和附着于贻贝粘合蛋白的生物活性物质。 贻贝粘合蛋白和生物活性物质涂在金属表面。 贻贝粘蛋白是由序列号4的氨基酸序列组成的蛋白质。

公开(公告)号：KR101233774B1

公开(公告)日：2013-02-22

申请号：KR1020100040307

申请日：2010-04-29

Applicant: 포항공과대학교 산학협력단 ,  주식회사 영웅알앤디센터

Inventor： 차형준 ,  황병희 ,  신화희

IPC: C12Q1/68 ,  C12Q1/04 ,  G01N33/569 ,  G01N33/58

CPC classification number: Y02A50/451 ,  Y02A50/52

Abstract: 본 발명은 병원성 미생물 탐지용 프로브 및 이를 이용한 살아 있는 병원성 미생물 검출방법에 관한 것으로, 구체적으로 본 발명은 기존의 표지 방식과는 달리 표지 물질을 포함하고 있는 ribosomal RNA의 탐지용 프로브의 개발과 이를 이용하여 임상 샘플 속의 살아 있는 병원성 미생물을 매우 신속하고 정확하게 검출할 수 있는 방법이다.

公开(公告)号：KR1020130002922A

公开(公告)日：2013-01-08

申请号：KR1020120023429

申请日：2012-03-07

Applicant: 포항공과대학교 산학협력단 ,  재단법인 포항산업과학연구원

Inventor： 차형준 ,  김임규 ,  강동균 ,  조병훈 ,  서정현

IPC: C12N15/60 ,  C12N9/88 ,  C12N15/70 ,  C12P3/00

CPC classification number: C12P9/00 ,  C12N9/88 ,  C12P3/00

Abstract: PURPOSE: A composition which captures carbon dioxide and a method for preparing carbonate using a recombinant whole cell biocatalyst is provided to economically capture carbon dioxide using cell homogenate supernatant or transformed cells and convert a final product into a high value-added carbonate. CONSTITUTION: A composition for capturing carbon dioxide contains: a whole cell of a transformant which is prepared by transforming with a vector containing a nucleic acid encoding recombinant carbonic anhydrase; a whole cell lysate or a fraction solution thereof; and the carbonic anhydrase isolated from the whole cells. The carbonic anhydrase is expressed in cytoplasm or a periplasmic space or on the cell surface. The carbonic anhydrase is derived from Neisseria gonorrhoeae, Synechocystis PCC6803, or Escherichia coli. The enzyme has an amino acid sequence of sequence number 1, 3, 5, 7, 9, 11, or 13. A nucleic acid encoding the carbonic anhydrase has a nucleic acid sequence of sequence number 2, 4, 6, 8, 10, 12, or 14.

Abstract translation: 目的：提供捕获二氧化碳的组合物和使用重组全细胞生物催化剂制备碳酸盐的方法，以便使用细胞匀浆上清液或转化细胞经济捕获二氧化碳，并将最终产物转化成高附加值碳酸盐。 构成：用于捕获二氧化碳的组合物包含：通过用含有编码重组碳酸酐酶的核酸的载体转化制备的转化体的全细胞; 全细胞裂解物或其级分溶液; 和从整个细胞分离的碳酸酐酶。 碳酸酐酶在细胞质或周质空间或细胞表面表达。 碳酸酐酶来自淋病奈瑟氏球菌，集胞藻PCC6803或大肠杆菌。 该酶具有序列号1,3,5,7,9,11或13的氨基酸序列。编码碳酸酐酶的核酸具有序列号2,4,6,8,10或10的核酸序列。 12或14。

公开(公告)号：KR1020120103435A

公开(公告)日：2012-09-19

申请号：KR1020120002161

申请日：2012-01-06

Applicant: 포항공과대학교 산학협력단

Inventor： 차형준 ,  양윤정 ,  최유성

IPC: C07K14/435 ,  C12N15/12 ,  C12N15/63 ,  D06M14/06

CPC classification number: C07K14/43504 ,  C07K14/43595 ,  D01D5/0007 ,  D01D5/06 ,  D01F4/00 ,  D10B2211/04

Abstract: PURPOSE: A sea anemone-derived recombinant silk protein and a composition containing the same for fabricating a silk fiber are provided to apply natural silk proteins with excellent intensity and elasticity. CONSTITUTION: A sea anemone-derived recombinant silk protein contains an amino acid sequence of sequence number 2. The protein contains an amino acid of sequence number 3. A method for preparing the proteins comprises: a step of preparing an expression vector containing a gene of the protein encoding the amino acid of sequence number 1; a step of inserting the expression vector to host cells to prepare a transformant; a step of producing the protein from the transformant; and a step of isolating and purifying the proteins. A composition for fabricating a silk fiber contains an amino acid sequence of sequence number 2, 3, or 4. [Reference numerals] (AA) Sea anemone-derived recombinant silk protein

Abstract translation: 目的：提供海葵衍生的重组丝蛋白和含有其的制备丝纤维的组合物以应用具有优异强度和弹性的天然丝蛋白。 构成：海葵衍生的重组丝蛋白含有序列号2的氨基酸序列。蛋白质含有序列号3的氨基酸。制备蛋白质的方法包括：制备含有基因的表达载体的步骤 编码序列号1的氨基酸的蛋白质; 将表达载体插入宿主细胞以制备转化体的步骤; 从转化体生产蛋白质的步骤; 以及分离和纯化蛋白质的步骤。 用于制造丝纤维的组合物含有序列号2,3或4的氨基酸序列。[AA]海葵衍生的重组丝蛋白

公开(公告)号：KR1020110051778A

公开(公告)日：2011-05-18

申请号：KR1020090108541

申请日：2009-11-11

Applicant: 포항공과대학교 산학협력단

Inventor： 김창섭 ,  차형준

IPC: C07K19/00 ,  G01N33/53 ,  C12N15/62 ,  C12N15/63

Abstract: PURPOSE: A novel fusion protein for a novel antibody fixation is provided to effectively attach to a solid substrate and to detecting an antigen. CONSTITUTION: A fusion protein for antibody fixation contains a mussel adhesive protein having an amino acid sequence of sequence number 6 with Staphylococcus aureus-derived protein A or an amino acid of protein A fragment and sequence number 6. The protein A has an amino acid of sequence number 7. A bio-chip for immune response contains the fusion protein for antibody fixation. A method for detecting an antibody comprises: a step of preparing a biochip containing the fusion protein; a step of fixing the antibody to an antigen; a step of reacting a sample to the bio-chip; and a step of measuring antigen-antibody reaction.

Abstract translation: 目的：提供一种用于新型抗体固定的新型融合蛋白，以有效附着于固体底物和检测抗原。 构成：用于抗体固定的融合蛋白包含具有序列号6的氨基酸序列的贻贝粘合蛋白，其具有来自金黄色葡萄球菌的蛋白A或蛋白A片段和序列号6的氨基酸。蛋白A具有 序列号7.用于免疫应答的生物芯片包含用于抗体固定的融合蛋白。 检测抗体的方法包括：制备含有融合蛋白的生物芯片的步骤; 将抗体固定在抗原上的步骤; 将样品与生物芯片反应的步骤; 和测定抗原 - 抗体反应的步骤。