재조합 생물촉매를 이용한 이산화탄소의 탄산염으로 전환 및 제조 방법
    2.
    发明公开
    재조합 생물촉매를 이용한 이산화탄소의 탄산염으로 전환 및 제조 방법 有权
    使用重组生物碳酸二氧化碳转化和生产碳酸钙的方法

    公开(公告)号:KR1020130002922A

    公开(公告)日:2013-01-08

    申请号:KR1020120023429

    申请日:2012-03-07

    CPC classification number: C12P9/00 C12N9/88 C12P3/00

    Abstract: PURPOSE: A composition which captures carbon dioxide and a method for preparing carbonate using a recombinant whole cell biocatalyst is provided to economically capture carbon dioxide using cell homogenate supernatant or transformed cells and convert a final product into a high value-added carbonate. CONSTITUTION: A composition for capturing carbon dioxide contains: a whole cell of a transformant which is prepared by transforming with a vector containing a nucleic acid encoding recombinant carbonic anhydrase; a whole cell lysate or a fraction solution thereof; and the carbonic anhydrase isolated from the whole cells. The carbonic anhydrase is expressed in cytoplasm or a periplasmic space or on the cell surface. The carbonic anhydrase is derived from Neisseria gonorrhoeae, Synechocystis PCC6803, or Escherichia coli. The enzyme has an amino acid sequence of sequence number 1, 3, 5, 7, 9, 11, or 13. A nucleic acid encoding the carbonic anhydrase has a nucleic acid sequence of sequence number 2, 4, 6, 8, 10, 12, or 14.

    Abstract translation: 目的:提供捕获二氧化碳的组合物和使用重组全细胞生物催化剂制备碳酸盐的方法,以便使用细胞匀浆上清液或转化细胞经济捕获二氧化碳,并将最终产物转化成高附加值碳酸盐。 构成:用于捕获二氧化碳的组合物包含:通过用含有编码重组碳酸酐酶的核酸的载体转化制备的转化体的全细胞; 全细胞裂解物或其级分溶液; 和从整个细胞分离的碳酸酐酶。 碳酸酐酶在细胞质或周质空间或细胞表面表达。 碳酸酐酶来自淋病奈瑟氏球菌,集胞藻PCC6803或大肠杆菌。 该酶具有序列号1,3,5,7,9,11或13的氨基酸序列。编码碳酸酐酶的核酸具有序列号2,4,6,8,10或10的核酸序列。 12或14。

    당류의 고정화 방법
    4.
    发明授权
    당류의 고정화 방법 失效
    碳水化合物的固定方法

    公开(公告)号:KR101101375B1

    公开(公告)日:2012-01-02

    申请号:KR1020090070661

    申请日:2009-07-31

    Abstract: 본 발명은 당류의 고정화 방법에 관한 것으로, 보다 구체적으로 당류를 고체 기재에 고정화시키는 방법 및 상기 고정화 방법에 의해 제작된 탄수화물 칩에 관한 것이다. 본 발명의 당류의 고정화 방법은 당류의 구조나 방향성에 변화를 주지 않고, 당류를 고체 기재에 직접 고정화할 수 있는 방법으로, 상기 방법으로 제조된 탄수화물 칩은 당류와 생체분자간의 상호작용을 용이하게 분석하여 맞춤형 바이오센서, 신약 개발 등 다양한 응용분양에 널리 사용될 수 있다.
    당류, 탄수화물, 고정화, 칩, 아민기, 아민

    당류의 고정화 방법
    5.
    发明公开
    당류의 고정화 방법 失效
    碳水化合物的固定方法

    公开(公告)号:KR1020110012794A

    公开(公告)日:2011-02-09

    申请号:KR1020090070661

    申请日:2009-07-31

    CPC classification number: G01N33/543 C12Q1/68

    Abstract: PURPOSE: A method for fixing saccharide on a solid substrate and a carbohydrate chip obtained thereby are provided to easily analyze interaction between the saccharide and biomolecule. CONSTITUTION: A method for fixing saccharide comprises: a step of reacting saccharides with reducing sugar and an amine compound to introduce an amine group(-NH_2) to the saccahrides; a step of fixing the saccharides on a solid substrate. The saccharide with reducing sugar is monosaccharide, bisaccharide, oligosaccharide or polysaccharide. The oligosaccharide is LS-tetrasacahride B or Lewis A trisaccharide.

    Abstract translation: 目的:提供一种将糖固定在固体基质上的方法和由此获得的碳水化合物芯片,以便于分析糖和生物分子之间的相互作用。 构成:用于固定糖的方法包括:使糖与还原糖和胺化合物反应以将氨基(-NH 2)引入到所述皂化物中的步骤; 将糖固定在固体基质上的步骤。 还原糖的糖是单糖,双糖,寡糖或多糖。 寡糖是LS-四沙巴酸B或路易斯A三糖。

    당류의 직접적인 표면 고정화 방법
    7.
    发明授权
    당류의 직접적인 표면 고정화 방법 失效
    固体基质表面上碳酸氢盐的直接固定方法

    公开(公告)号:KR100804713B1

    公开(公告)日:2008-02-18

    申请号:KR1020060105908

    申请日:2006-10-30

    CPC classification number: A61K49/00 C07H15/18

    Abstract: A method for immobilizing saccharide on the surface of a substrate is provided to immobilize directly the saccharide on the surface of a solid substrate without changing the structure of the saccharide and is applied to various saccharides. A method for immobilizing saccharide on the surface of a substrate such as gold comprises the steps of: (a) reacting an aldehyde of the saccharide selected from the group consisting of reducing monosaccharide, oligosaccharide having a terminal reducing sugar, and polysaccharide with an amine group of an amine compound represented by NH2-R-SH(where R is CC30-50 alkylene, C6-30 aryl or C6-30 aryl including C10-30 alkylene) to prepare a thiol group bound saccharide; and coating the thiol group bound saccharide to the surface of the substrate to bind the thio to the substrate surface, where when the saccharide is a monosaccharide, the reaction is done at a temperature of 20-90 deg.C for 0.5-2 hours, and when the saccharide is a polysaccharide, the reaction is done at a temperature of 30-90 deg.C for 0.5-2 hours. The method further comprises a step of reducing the thiol bound saccharide.

    Abstract translation: 提供了将糖固定在基材表面上的方法,用于将糖直接固定在固体基质的表面上,而不改变糖的结构并施加到各种糖上。 使糖类固定在基底如金的表面上的方法包括以下步骤:(a)将选自还原性单糖,末端还原糖的寡糖与多糖的糖类与胺基反应 由NH2-R-SH(其中R是CC30-50亚烷基,C6-30芳基或包括C10-30亚烷基的C6-30芳基)表示的胺化合物以制备巯基结合的糖; 并将硫醇结合的糖包被到底物的表面以将硫结合到底物表面,其中当糖是单糖时,反应在20-90℃的温度下进行0.5-2小时, 当糖是多糖时,反应在30-90℃的温度下进行0.5-2小时。 该方法还包括还原硫醇结合的糖的步骤。

    표면 비의존적 항균 코팅용 생체접착제
    8.
    发明公开
    표면 비의존적 항균 코팅용 생체접착제 有权
    表面独立的抗菌涂层生物

    公开(公告)号:KR1020150112318A

    公开(公告)日:2015-10-07

    申请号:KR1020140036225

    申请日:2014-03-27

    CPC classification number: C07K19/00 A61L27/34 B82B1/00 C12N15/09

    Abstract: 본발명은홍합접착단백질에금속결합펩타이드가연결된융합단백질, 상기융합단백질에금속이결합되어형성된금속나노입자를포함하며항균활성과접착성을갖는단백질-금속나노입자접착성복합체및 이의제조방법, 상기복합체를포함하는표면코팅제및 표면코팅방법에관한것이다.

    Abstract translation: 本发明涉及将金属结合肽结合到贻贝粘合蛋白上的融合蛋白; 涉及具有粘合性和抗菌活性的蛋白质 - 金属纳米颗粒粘合剂复合物及其制造方法,其包含通过将金属结合到所述融合蛋白上而制备的金属纳米粒子。 和表面涂布法,以及包含该复合物的表面涂层剂。

    항체의 방향성 표면 고정화 방법
    9.
    发明公开
    항체의 방향성 표면 고정화 방법 无效
    基质表面抗体的定向固定方法

    公开(公告)号:KR1020090131576A

    公开(公告)日:2009-12-29

    申请号:KR1020080057518

    申请日:2008-06-18

    Abstract: PURPOSE: A method for fixing antibody on a substrate to have constant direction is provided to improve stability, specificity and sensitivity of immunosensor. CONSTITUTION: A method for fixing an antibody on a substrate surface to have direction comprises: a step of introducing cystein residue to terminal of Fc-binding peptide; a step of introducing Fc-binding peptide on the substrate surface; and a step of fixing the antibody to the other terminal of Fc-binding peptide. The Fc-binding peptide is His-Xaa1-Xaa2-Xaa3-Xaa4-Xaa5. The Xaa1 is aromatic amino acid or histidine residue. The Xaa2 is potisively charged amino acid or hydrophobic amino acid. The Xaa3, Xaa4, and Xaa5 can be any amino acid.

    Abstract translation: 目的:提供一种将抗体固定在基质上以保持恒定方向的方法,以提高免疫传感器的稳定性,特异性和灵敏度。 构成:将抗体固定在基材表面以具有方向的方法包括:将半胱氨酸残基引入Fc结合肽的末端的步骤; 将Fc结合肽引入基片表面的步骤; 以及将抗体固定在Fc结合肽的另一个末端的步骤。 Fc结合肽是His-Xaa1-Xaa2-Xaa3-Xaa4-Xaa5。 Xaa1是芳香族氨基酸或组氨酸残基。 Xaa2是带电荷的氨基酸或疏水性氨基酸。 Xaa3,Xaa4和Xaa5可以是任何氨基酸。

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