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公开(公告)号：KR101569164B1
公开(公告)日：2015-11-13
申请号：KR1020140078590
申请日：2014-06-26
Applicant: 한국화학연구원
Abstract: 본발명은 PHA 검출용조성물, 이를이용한 PHA 염색방법, PHA 검출방법, PHA 합성관련유전자의스크리닝방법, 및배양조건변화에따른고수율의 PHA 생산방법에관한것으로, 시료에포함된 PHA만을특이적으로염색시켜 PHA의유무를판별하고 PHA의양을정량적으로측정할수 있으며, 미생물또는식물의성장을저해하거나미생물고정, 세포벽을파괴하기위한별도의전처리없이도살아있는생물체에서 PHA 합성과동시에 PHA의양을측정할수 있어, PHA 고생산성균주또는식물개발을위한유전자라이브러리의고속대량검출(High Throughput Screening)에활용할수 있다.
Abstract translation: 本发明涉及用于检测聚羟基链烷酸酯（PHA）的组合物，使用其的PHA染色方法，PHA检测方法，用于筛选与PHA合成相关的基因的方法，以及根据 培养条件的变化。 根据本发明，本发明的组合物确保PHA含量的定量测定，并且通过专门染色样品中所含的PHA来测定PHA的存在。 此外，组合物使得可以在活体中合成PHA的同时测量PHA的量，而不需要额外的预处理来抑制微生物或植物的生长，固定微生物和破坏细胞壁，从而适用于高通量筛选 用于开发高度产生PHA的菌株或植物的基因文库。
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42.发明授权
公开(公告)号：KR101466614B1
公开(公告)日：2014-12-02
申请号：KR1020130012562
申请日：2013-02-04
Applicant: 한국화학연구원
IPC: C07C231/22 , C07C215/76 , C12N9/00
Abstract: 본발명은메타게놈으로부터분리한서열번호 1로표시되는아미노산서열을갖는하이드록실아미노벤젠뮤타아제를이용하여다양한아미노페놀유도체를제조하는방법에관한것으로서, 본발명의방법은의약중간체, 각종기능성화학물질또는중간물질로사용될수 있는다양한아미노페놀유도체를제조하는데유용하게사용될수 있다.
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公开(公告)号：KR101394467B1
公开(公告)日：2014-05-14
申请号：KR1020120154907
申请日：2012-12-27
Applicant: 한국화학연구원
Abstract: The present invention relates to a novel hydroxylaminobenzene mutase and a gene coding the same, and a hydroxylaminobenzene mutase catalyzes reaction which rearranges a hydroxyl group in a hydroxylamino compound, thereby being expected to be used for manufacturing pharmaceutical intermediates, various kinds of functional chemical substances, or intermediates.
Abstract translation: 本发明涉及一种新的羟基氨基苯变异酶及其编码基因，羟基氨基苯变异酶催化羟基氨基化合物中的羟基重排的反应，预期用于制备药物中间体，各种功能性化学物质， 或中间体。
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Patent Agency Ranking
公开(公告)号：KR1020110060384A
公开(公告)日：2011-06-08
申请号：KR1020090116957
申请日：2009-11-30
Applicant: 한국화학연구원 , 충남대학교산학협력단
Abstract: PURPOSE: A Candida Antarctica-derived lipase B(CalB) protein variant is provided to increase enzyme activity and to be used in the industry. CONSTITUTION: A CalB protein variant is selected from a protein of amino acid sequences 2-8 in which 274-289th amino acid is partially substituted in Candida Antarctica-derived lipase B(CalB) protein. A gene encoding CalB protein variant sequence is denoted by sequence number selected from sequence numbers 10-16. A recombinant vector containing the gene is prepared by inserting the gen into pK7 vector. A transformed cell which produces CalB protein variant is obtained by transforming a host cell with the recombinant vector. The host cells are Pichia pastoris.
Abstract translation: 目的：提供来自南极假丝酵母的脂肪酶B（CalB）蛋白质变体以增加酶活性并用于该行业。 构成：CalB蛋白质变体选自氨基酸序列2-8的蛋白质，其中274-289位氨基酸被部分取代在南极假单胞菌来源的脂肪酶B（CalB）蛋白质中。 编码CalB蛋白变体序列的基因由选自序列号10-16的序列号表示。 通过将gen插入pK7载体中制备含有该基因的重组载体。 通过用重组载体转化宿主细胞获得产生CalB蛋白质变体的转化细胞。 宿主细胞是巴斯德毕赤酵母。
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公开(公告)号：KR1020100072848A
公开(公告)日：2010-07-01
申请号：KR1020080131375
申请日：2008-12-22
Applicant: 한국화학연구원
Abstract: PURPOSE: A surface expression vector of a foreign protein containing C-terminal deleted OmpW is provided to use in protein array, antibody manufacture, bioconversion, and foreign protein improvement. CONSTITUTION: A surface expression vector of a foreign protein contains C-terminal deleted OmpW and foreign protein gene. In OmpW, C-terminal which is downstream of 139th amino acid and downstream of 191th amino acid is deleted. A transformant is prepared by transducing the surface expression vector of a foreign protein into a host cell. The host cell is E.coli. A method for preparing antibody using the cell on which foreign protein is expressed comprises: a step of administering the cell to the host cell to induce immune response; and a step of collecting produced antibody in an individual.
Abstract translation: 目的：提供含有C-末端缺失OmpW的外源蛋白的表面表达载体，用于蛋白质阵列，抗体制备，生物转化和外源蛋白质改良。 构成：外来蛋白质的表面表达载体含有C末端缺失的OmpW和外来蛋白质基因。 在OmpW中，缺失了第139位氨基酸下游和第191位氨基酸下游的C末端。 通过将外源蛋白质的表面表达载体转导入宿主细胞来制备转化体。 宿主细胞是大肠杆菌。 使用表达外源蛋白质的细胞制备抗体的方法包括：向宿主细胞施用细胞以诱导免疫应答的步骤; 以及在个体中收集产生的抗体的步骤。
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公开(公告)号：KR1020060126084A
公开(公告)日：2006-12-07
申请号：KR1020050047709
申请日：2005-06-03
Applicant: 한국화학연구원
IPC: C08G59/62 , C09D163/00 , C09D161/08
Abstract: A method for preparing a natural phenolic epoxy resin is provided to obtain a phenolic epoxy resin useful as a solvent-free coating agent having excellent water resistance, acid resistance, alkali resistance and stability to an organic solvent. The method for preparing a natural phenolic epoxy resin comprises the steps of: forming an unsaturated double bond-containing phenolic polymer derived from plants; reacting an organic acid, hydrolase and hydrogen peroxide to form a peroxidized organic acid; and inserting oxygen atom into the unsaturated double bond of the plant-derived phenolic polymer by using the peroxidized organic acid. Particularly, the peroxidized organic acid is obtained by dissolving the phenolic polymer and the organic acid into an organic solvent, and introducing hydrolase and hydrogen peroxide thereto so that hydrogen peroxide is decomposed by hydrolase to provide oxygen, which, in turn, is bound to the organic acid.
Abstract translation: 提供一种制备天然酚醛环氧树脂的方法，以获得可用作无机涂料的酚醛环氧树脂，其具有优异的耐水性，耐酸性，耐碱性和对有机溶剂的稳定性。 制备天然酚醛环氧树脂的方法包括以下步骤：形成由植物衍生的含不饱和双键的酚类聚合物; 使有机酸，水解酶和过氧化氢反应形成过氧化的有机酸; 并通过使用过氧化的有机酸将氧原子插入到植物来源的酚类聚合物的不饱和双键中。 特别地，过氧化有机酸是通过将酚类聚合物和有机酸溶解在有机溶剂中，并且向其中引入水解酶和过氧化氢以使过氧化氢通过水解酶分解以提供氧而获得的，其又结合到 有机酸。
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公开(公告)号：KR100644267B1
公开(公告)日：2006-11-10
申请号：KR1020050071606
申请日：2005-08-05
Applicant: 한국화학연구원
Abstract: A flotation de-inking method of waste paper with an improved yield is provided to perform the enzyme treatment of the first flotation reject powder. A hydrophobic material is concentrated after the floating process. The concentrated flotation reject is separated and discharged. One or more enzymes selected from a group consisting of esterase, lipase or cutinase are injected into the entire waste paper in a Froth-Flotation Cell and the only adsorbed material is selectively modified into hydrophilic material. The addition amount of the enzyme is 0.02-2 weight % relating to the dry weight of the entire waste paper. The hydrophobic material is the functional additive or cohesive foreign substance.
Abstract translation: 提供具有提高产率的废纸的浮选脱墨方法以对第一浮选废料粉进行酶处理。 漂浮过程后疏水材料被浓缩。 浓缩的浮选废料被分离并排出。 将一种或多种选自酯酶，脂肪酶或角质酶的酶注入到泡沫浮选池中的整个废纸中，并将唯一吸附的材料选择性地修饰成亲水材料。 酶的添加量相对于整个废纸的干重为0.02-2重量％。 疏水性材料是功能性添加剂或粘性外来物质。
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公开(公告)号：KR1020060064922A
公开(公告)日：2006-06-14
申请号：KR1020040103608
申请日：2004-12-09
Applicant: 한국화학연구원
CPC classification number: C08G65/38 , B01J31/04 , B01J2531/72 , B01J2531/845 , C08G65/44
Abstract: 본 발명은 코프리너스 시네레우스 유래 퍼옥시다아제를 이용한 페놀계 고분자의 제조방법에 관한 것으로서, 더욱 상세하게는 페놀 단량체 중합시 유독성의 포르말린 사용이 필수적인 통상의 퍼옥시다아제와 가격이 매우 고가이고 과산화수소에 의하여 쉽게 불활성화되는 식물체 유래 퍼옥시다아제 대신에, 코프리너스 시네레우스 유래 퍼옥시다아제를 이용하여 페놀계 단량체를 극성 유기용매 내에서 온화한 반응조건(상온, 상압) 하에서 중합시킴으로써 수율이 증대되고, 경제적이며, 내수성, 내화학성이 뛰어난 페놀계 고분자를 제조하는 방법에 관한 것이다.
퍼옥시다아제, 페놀계 고분자, 코프리너스 시네레우스
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