公开(公告)号：KR1020120097859A

公开(公告)日：2012-09-05

申请号：KR1020110017356

申请日：2011-02-25

Applicant: 한국화학연구원

Inventor： 윤석주 ,  황승용 ,  오정화 ,  박한진 ,  김승준 ,  오문주

IPC: C12Q1/68 ,  C12N15/12 ,  C40B40/08

CPC classification number: C12Q1/6888 ,  C12Q1/6837 ,  C12Q2600/142 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: PURPOSE: A microarray chip for detecting reproductive toxicant induced by endocrine disruptor and a kit for detecting reproductive toxicant are provided to identify reproductive toxicity mechanism and to study ecotoxicogenomics. CONSTITUTION: A microarray chip for detecting reproductive toxicant induced by endocrine disruptor contains a biomarker. The biomarker is an oligonucleotide containing 15-50 nucleic acids or complementary strand molecule thereof. A method for detecting the biomarker to identify reproductive toxicity induced by endocrine disruptor comprises: a step of synthesizing cDNA from RNA samples isolated from regenerative cells of an experimental group(male individual which is suspicious for endocrine disruptor exposure) and a control group; a step of labeling the cDNA with different fluorescent substances; a step of hybridizing the cDNA with the DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing gene expression level of the DNA microarray chip between the experimental group and control group. [Reference numerals] (AA) Reproduction; (BB) Cell proliferation; (CC) Signal transduction; (DD) Cell adhesion; (EE) Cell cycle; (FF) Immune response; (GG) Inflammatory response; (HH) Cell apoptosis; (II) Transport; (JJ) Lipid metabolism; (KK) Protein synthesis; (LL) Gene transcription; (MM) Homeostasis; (NN) Cell growth; (OO) Cell differentiation

Abstract translation: 目的：提供用于检测内分泌干扰物引起的生殖毒素的微阵列芯片和用于检测生殖毒物的试剂盒，以鉴定生殖毒性机制和研究生态毒性基因组学。 构成：用于检测内分泌干扰物引起的生殖毒物的微阵列芯片含有生物标志物。 生物标志物是含有15-50个核酸或其互补链分子的寡核苷酸。 用于检测生物标志物以鉴定由内分泌干扰物引起的生殖毒性的方法包括：从实验组（对于内分泌干扰物暴露的可疑的雄性个体）的再生细胞分离的RNA样品和对照组合成cDNA的步骤; 用不同荧光物质标记cDNA的步骤; 将cDNA与DNA微阵列芯片杂交的步骤; 分析DNA微阵列芯片的步骤; 并比较实验组与对照组之间的DNA芯片芯片的基因表达水平。 （附图标记）（AA）繁殖; （BB）细胞增殖; （CC）信号转导; （DD）细胞粘附; （EE）细胞周期; （FF）免疫反应; （GG）炎症反应; （HH）细胞凋亡; （二）运输; （JJ）脂质代谢; （KK）蛋白质合成; （LL）基因转录; （MM）稳态; （NN）细胞生长; （OO）细胞分化

公开(公告)号：KR1020110069448A

公开(公告)日：2011-06-23

申请号：KR1020090126188

申请日：2009-12-17

Applicant: 한국화학연구원

Inventor： 윤석주 ,  황승용 ,  김승준 ,  오문주 ,  오정화 ,  박한진

IPC: C12Q1/68 ,  C12N15/12 ,  C12N5/076

CPC classification number: C12Q1/6837 ,  C12N5/061 ,  C12N15/11 ,  C12Q1/6827

Abstract: PURPOSE: A method for detecting bisphenol A using reproductive system chromosome aberration is provided to determine bisphenol A presence in a test sample. CONSTITUTION: A method for detecting bisphenol A(BPA) of genetic toxic level in a test sample comprises: a step of isolating genomic DNA from reproductive cells of a male experimental animal except for human body; a step of measuring copy number from the genomic DNA; and a step of confirming copy number variation(CNV) in the genome. The reproductive cell is derived from testis or epididymis. The animal is rat, mouse, rabbit, guinea pig, hamster, dog, or cat. In case of a rat or mouse, the CNV exists in Chr2:77739470-77848433, Chr5:128329169-128329869, Chr7:7312289-10272836, Chr9:101951419-101951819, Chr10:72178606-72179006, Chr12:17803411-25366481, or Chr14:4551029-10397399. A gene relating to pheromone reaction is Vomeronasal 1 receptor, J2, E11, L1, G2, G3, G4, G5, G6, G7, G11, or K1.

Abstract translation: 目的：提供使用生殖系统染色体畸变检测双酚A的方法，以确定测试样品中双酚A的存在。 构成：测试样品中检测双酚A（BPA）的遗传毒性水平的方法包括：从除人体外的雄性实验动物的生殖细胞中分离基因组DNA的步骤; 从基因组DNA测量拷贝数的步骤; 以及确认基因组中拷贝数变异（CNV）的步骤。 生殖细胞来源于睾丸或附睾。 动物是大鼠，小鼠，兔，豚鼠，仓鼠，狗或猫。 在大鼠或小鼠的情况下，CNV存在于Chr2：77739470-77848433，Chr5：128329169-128329869，Chr7：7312289-10272836，Chr9：101951419-101951819，Chr10：72178606-72179006，Chr12：17803411-25366481或Chr14 ：4551029-10397399。 与信息素反应相关的基因是犁鼻1受体，J2，E11，L1，G2，G3，G4，G5，G6，G7，G11或K1。

公开(公告)号：KR1020090122714A

公开(公告)日：2009-12-01

申请号：KR1020080048660

申请日：2008-05-26

Applicant: 한국화학연구원

Inventor： 윤석주 ,  수레쉬브이.에스.라나 ,  오정화 ,  박한진 ,  김충용 ,  한상섭

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6837 ,  C12Q2600/142

Abstract: PURPOSE: A biomarker for diagnosing hepatotoxicity induced by benzene and a method for diagnosing hepatotoxicity are provided to study mechanism of hepatotoxicity and leukemia induced by exposure of benzene and other various chemicals. CONSTITUTION: A biomarker for diagnosing hepatotoxicity induced by benzene is a gene of which expression is increased or decreased by exposure of a hepatotoxicity chemical. The hepatotoxicity chemical is benzene. A method for diagnosing hepatotoxicity comprises: a step of isolating RNA from hepatocytes of experimental group of patient and control group; a step of synthesizing cDNA of the experimental group and control group; a step of synthesizing cDNA to cRNA a step of hybridizing using cRNA with microarray; a step of analyzing microarray; and a step of comparing results of control group and experimental group.

Abstract translation: 目的：提供苯诊断肝毒性的生物标志物和肝毒性诊断方法，用于研究苯和其他各种化学物质暴露引起的肝毒性和白血病的机制。 构成：用于诊断苯诱导的肝毒性的生物标志物是通过暴露于肝毒性化学物质来表达增加或减少的基因。 肝毒性化学物质为苯。 一种诊断肝毒性的方法包括：从实验组和对照组的肝细胞中分离RNA的步骤; 合成实验组和对照组cDNA的步骤; 将cDNA与cRNA合成的步骤是使用cRNA与微阵列杂交的步骤; 分析微阵列的一个步骤; 比较对照组和实验组的比较结果。

公开(公告)号：KR100636425B1

公开(公告)日：2006-10-19

申请号：KR1020040096845

申请日：2004-11-24

Applicant: 한국화학연구원

Inventor： 송창우 ,  조규혁 ,  조재우 ,  남윤이 ,  윤석주 ,  한상섭

IPC: A01K67/027

Abstract: 본 발명은 에틸니트로소우레아(N
- ethyl-N-nitrosourea, ENU)를 이용한 화학적 돌연변이(chemical mutagenesis)에 의해 백내장이 발생되어 수정체 후방이 돌출되고 돌출된 부분 주위로 고리모양의 백탁이 형성되는 표현형을 갖는 백내장 돌연변이 마우스에 관한 것으로, 본 발명에 따른 백내장 돌연변이 마우스는 복잡한 유전자적 조작없이 ENU의 처리에 의해 용이하게 획득이 가능하고, 기존의 백내장 돌연변이 마우스와 구별되는 표현형을 가지며, 백내장 돌연변이가 상염색체성 열성 유전 양식에 따라 안정적으로 후세대에 전달되므로, 인간 백내장의 발병 기전연구 및 백내장 치료제 개발을 위한 생체내(
in vivo ) 스크리닝 모델동물로서 유용하게 사용될 수 있다.