公开(公告)号：AU1138902A

公开(公告)日：2002-04-15

申请号：AU1138902

申请日：2001-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： CHOU HOU-PU ,  EYAL SHULAMIT ,  FU ANNE Y ,  QUAKE STEPHEN R

IPC: B01L99/00 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  F04B11/00 ,  F04B19/00 ,  F04B43/04 ,  G01N15/14 ,  G01N30/20 ,  G01N30/32 ,  G01N30/60 ,  C12Q1/68

Abstract: A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.

公开(公告)号：NO20016268D0

公开(公告)日：2001-12-20

申请号：NO20016268

申请日：2001-12-20

Applicant: CALIFORNIA INST OF TECHN

Inventor： UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R

IPC: G03F7/20 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  B81B7/00 ,  B81C1/00 ,  B81C99/00 ,  C12Q1/68 ,  F04B17/00 ,  F04B35/04 ,  F04B43/04 ,  F15C5/00 ,  F16K7/00 ,  F16K17/02 ,  F16K99/00 ,  F16K

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer, bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

公开(公告)号：AU7534001A

公开(公告)日：2001-12-17

申请号：AU7534001

申请日：2001-06-05

Applicant: CALIFORNIA INST OF TECHN

Inventor： QUAKE STEPHEN R ,  CHOU HOU-PU

IPC: G01N21/64 ,  B01F5/06 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L99/00 ,  B81B1/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/05 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/58 ,  G01N37/00 ,  B01J19/00

Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.