公开(公告)号：WO0229106A3

公开(公告)日：2002-07-11

申请号：PCT/US0130933

申请日：2001-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： CHOU HOU-PU ,  EYAL SHULAMIT ,  FU ANNE Y ,  QUAKE STEPHEN R

IPC: B01L99/00 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  F04B11/00 ,  F04B19/00 ,  F04B43/04 ,  G01N15/14 ,  G01N30/20 ,  G01N30/32 ,  G01N30/60

CPC classification number: F04B43/00 ,  A61M2206/22 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  F04B11/00 ,  F04B19/006 ,  F04B43/043 ,  G01N30/6095 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2030/205 ,  G01N2030/322 ,  Y10T436/2575

Abstract: A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality. Velocity independent cytometry methods and apparatuses are also described.

Abstract translation: 微流体装置包括泵，阀和流体振荡阻尼器。 在用于分选的装置中，实体通过泵沿着流动通道流过检测区域到达连接点。 基于在检测区域中确定的实体的身份，致动位于流动通道的相对分支处的废物或收集阀，由此将实体路由到废物池或收集池。 阻尼器结构可以位于泵和连接点之间。 阻尼器减小了由于泵的操作而引起的流动通道中的振荡压力的幅度，从而减小了分选过程中实体的速度振荡。 微流体装置可以形成为弹性体材料块，弹性体材料的薄膜可偏转到流动通道中以提供泵或阀功能。 还描述了速度非依赖性细胞计数方法和装置。

公开(公告)号：AU1138902A

公开(公告)日：2002-04-15

申请号：AU1138902

申请日：2001-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： CHOU HOU-PU ,  EYAL SHULAMIT ,  FU ANNE Y ,  QUAKE STEPHEN R

IPC: B01L99/00 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  F04B11/00 ,  F04B19/00 ,  F04B43/04 ,  G01N15/14 ,  G01N30/20 ,  G01N30/32 ,  G01N30/60 ,  C12Q1/68

Abstract: A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.