公开(公告)号：US6408878B2

公开(公告)日：2002-06-25

申请号：US79666601

申请日：2001-02-28

Applicant: CALIFORNIA INST OF TECHN

Inventor： UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R

IPC: G03F7/20 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  B81B7/00 ,  B81C1/00 ,  B81C99/00 ,  C12Q1/68 ,  F04B17/00 ,  F04B35/04 ,  F04B43/04 ,  F15C5/00 ,  F16K7/00 ,  F16K17/02 ,  F16K99/00 ,  F16K11/20

CPC classification number: B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

公开(公告)号：EP1343973A4

公开(公告)日：2004-12-22

申请号：EP01990762

申请日：2001-11-16

Applicant: CALIFORNIA INST OF TECHN

Inventor： QUAKE STEPHEN R ,  UNGER MARC ALEXANDER ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL

IPC: B01D57/02 ,  B01D61/18 ,  B01D61/28 ,  B01D63/08 ,  B01F5/06 ,  B01F7/00 ,  B01F11/00 ,  B01F13/00 ,  B01F13/10 ,  B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  F04B43/04 ,  F15C5/00 ,  F16K99/00 ,  G01N27/447 ,  G01N33/50 ,  F15C1/04 ,  C12M1/34 ,  C12Q1/68 ,  F15C1/06

CPC classification number: G01N33/00 ,  B01D57/02 ,  B01D61/18 ,  B01D61/28 ,  B01D63/081 ,  B01D63/088 ,  B01D2313/08 ,  B01D2313/90 ,  B01F5/0646 ,  B01F5/0647 ,  B01F7/00 ,  B01F11/0071 ,  B01F13/0059 ,  B01F13/1013 ,  B01F13/1022 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/082 ,  C12M23/16 ,  C12M41/46 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N15/06 ,  G01N27/44743 ,  G01N27/44791 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/56966 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

公开(公告)号：WO0243615A3

公开(公告)日：2003-03-13

申请号：PCT/US0144549

申请日：2001-11-28

Applicant: CALIFORNIA INST OF TECHN

Inventor： UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R ,  LIU JIAN ,  ADAMS MARK L ,  HANSEN CARL L

IPC: B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  B67D99/00 ,  B81B1/00 ,  B81B3/00 ,  B81C1/00 ,  C12Q1/68 ,  F04B43/04 ,  F15C5/00 ,  F16K7/00 ,  F16K31/126 ,  F16K99/00 ,  A61F2/00

CPC classification number: B05D3/04 ,  B01J2219/00355 ,  B01J2219/00378 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00439 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/06 ,  B01L2400/0655 ,  B01L2400/0688 ,  B32B2037/1081 ,  B65B31/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  C12Q1/6832 ,  C12Q1/6874 ,  C30B29/54 ,  F04B43/043 ,  F15C1/06 ,  F15C3/00 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0324 ,  Y10T137/0379 ,  Y10T137/0424 ,  Y10T137/206 ,  Y10T137/2082 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2174 ,  Y10T137/2202 ,  Y10T137/2224 ,  Y10T137/3084 ,  Y10T137/7837 ,  Y10T137/86027 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24612 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

公开(公告)号：WO0194635A9

公开(公告)日：2002-08-08

申请号：PCT/US0118400

申请日：2001-06-05

Applicant: CALIFORNIA INST OF TECHN ,  QUAKE STEPHEN R ,  CHOU HOU-PU

Inventor： QUAKE STEPHEN R ,  CHOU HOU-PU

IPC: G01N21/64 ,  B01F5/06 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L99/00 ,  B81B1/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/05 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/58 ,  G01N37/00 ,  B01J19/00

CPC classification number: B01L3/02 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0072 ,  B01F13/0093 ,  B01F15/0201 ,  B01F15/024 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0694 ,  C12M1/02 ,  C12Q1/6816 ,  G01N33/53 ,  Y10T137/0318 ,  Y10T137/0329 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T436/11 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/2575 ,  C12Q2565/629

Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Abstract translation: 本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其他分子的微制造装置。 本发明的装置和方法可以用于通过检测分子（例如分子数量），例如多核苷酸（例如DNA）或蛋白质（例如抗体），通过测量可检测信号来同时诊断多种疾病 报道子与杂交的多核苷酸或抗原/抗体复合物相关。 在根据本发明的微制造装置中，检测分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在与来自与结合分子相关的光学可检测（例如荧光）报道分子的杂交信号相关。 这些杂交信号可以通过任何合适的手段检测，例如光学检测，并且可以例如作为特定基因存在的表示存储在计算机中。 可以将杂交探针固定在形成一部分或暴露于形成闭合环路的装置的一个或多个通道的基底上，以使样品循环以主动接触互补探针。 根据本发明的通用芯片不仅可以用DNA制造，而且可以用其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子制造。

公开(公告)号：WO0101025A2

公开(公告)日：2001-01-04

申请号：PCT/US0017740

申请日：2000-06-27

Applicant: CALIFORNIA INST OF TECHN ,  UNGER MARC A ,  CHOU HOU PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R

Inventor： UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R

IPC: G03F7/20 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  B81B7/00 ,  B81C1/00 ,  B81C99/00 ,  C12Q1/68 ,  F04B17/00 ,  F04B35/04 ,  F04B43/04 ,  F15C5/00 ,  F16K7/00 ,  F16K17/02 ,  F16K99/00 ,  F16K17/00

CPC classification number: B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising : forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微机械模具的顶部上形成第一弹性体层，所述第一微机械模具具有第一凸起突起，所述第一凸起形成沿所述第一弹性体层的底表面延伸的第一凹陷; 在第二微机械模具的顶部上形成第二弹性体层，所述第二微机械模具具有第二凸起突起，所述第二凸起形成沿着所述第二弹性体层的底表面延伸的第二凹陷; 将所述第二弹性体层的底表面结合到所述第一弹性体层的顶表面上，使得在所述第一和第二弹性体层之间的所述第二凹陷中形成控制通道; 以及将第一弹性体层定位在平面基底的顶部上，使得在第一弹性体层和平面基底之间的第一凹陷中形成流动通道。

公开(公告)号：WO0194635A3

公开(公告)日：2002-10-31

申请号：PCT/US0118400

申请日：2001-06-05

Applicant: CALIFORNIA INST OF TECHN ,  QUAKE STEPHEN R ,  CHOU HOU-PU

Inventor： QUAKE STEPHEN R ,  CHOU HOU-PU

IPC: G01N21/64 ,  B01F5/06 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L99/00 ,  B81B1/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/05 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/58 ,  G01N37/00 ,  B01F5/10 ,  B01J19/00

CPC classification number: B01L3/02 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0072 ,  B01F13/0093 ,  B01F15/0201 ,  B01F15/024 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0694 ,  C12M1/02 ,  C12Q1/6816 ,  G01N33/53 ,  Y10T137/0318 ,  Y10T137/0329 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T436/11 ,  Y10T436/145555 ,  Y10T436/173845 ,  Y10T436/2575 ,  C12Q2565/629

Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

Abstract translation: 本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其它分子的微制造装置。 本发明的装置和方法可用于通过检测分子（例如数量的分子），例如多核苷酸（例如DNA）或蛋白质（例如抗体）来同时诊断多种疾病。 与杂交多核苷酸或抗原/抗体复合物相关的报道。 在根据本发明的微制造装置中，分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在的检测与来自与结合分子相关的光学可检测（例如荧光）报告物的杂交信号相关。 这些杂交信号可以通过任何合适的方式，例如光学检测，并且可以例如存储在计算机中作为特定基因的存在的表示。 杂交探针可以固定在形成一部分或暴露于形成闭环的装置的通道或通道的基底上，用于循环样品以主动接触互补探针。 根据本发明的通用芯片不仅可以用DNA而且可以与其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子一起制备。

公开(公告)号：WO0175176A3

公开(公告)日：2003-08-07

申请号：PCT/US0110881

申请日：2001-04-04

Applicant: CALIFORNIA INST OF TECHN ,  QUAKE STEPHEN R ,  CHOU HOU-PU

Inventor： QUAKE STEPHEN R ,  CHOU HOU-PU

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/683

Abstract: This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacteria typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.

Abstract translation: 本发明一般涉及分子指纹图谱的方法。 该方法可用于法医鉴定（例如DNA指纹图谱，特别是VNTR），细菌分型和人/动物病原体诊断。 更具体地，可以按照限制性片段长度多态性分析多核苷酸的微制造装置中的大小来评估或分类分子，例如多核苷酸（例如DNA）。 在根据本发明的微制造装置中，通过测量例如与多核苷酸片段相关的可光学检测（例如，荧光）报告基因的信号，可以使用相对小的样品快速和精确地分类DNA片段或其他分子。

公开(公告)号：WO0101025A9

公开(公告)日：2002-07-25

申请号：PCT/US0017740

申请日：2000-06-27

Applicant: CALIFORNIA INST OF TECHN ,  UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R

Inventor： UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R

IPC: G03F7/20 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  B81B7/00 ,  B81C1/00 ,  B81C99/00 ,  C12Q1/68 ,  F04B17/00 ,  F04B35/04 ,  F04B43/04 ,  F15C5/00 ,  F16K7/00 ,  F16K17/02 ,  F16K99/00

CPC classification number: B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising : forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

公开(公告)号：WO0229106A3

公开(公告)日：2002-07-11

申请号：PCT/US0130933

申请日：2001-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： CHOU HOU-PU ,  EYAL SHULAMIT ,  FU ANNE Y ,  QUAKE STEPHEN R

IPC: B01L99/00 ,  B01L3/00 ,  B81B1/00 ,  B81B3/00 ,  F04B11/00 ,  F04B19/00 ,  F04B43/04 ,  G01N15/14 ,  G01N30/20 ,  G01N30/32 ,  G01N30/60

CPC classification number: F04B43/00 ,  A61M2206/22 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  F04B11/00 ,  F04B19/006 ,  F04B43/043 ,  G01N30/6095 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2030/205 ,  G01N2030/322 ,  Y10T436/2575

Abstract: A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality. Velocity independent cytometry methods and apparatuses are also described.

Abstract translation: 微流体装置包括泵，阀和流体振荡阻尼器。 在用于分选的装置中，实体通过泵沿着流动通道流过检测区域到达连接点。 基于在检测区域中确定的实体的身份，致动位于流动通道的相对分支处的废物或收集阀，由此将实体路由到废物池或收集池。 阻尼器结构可以位于泵和连接点之间。 阻尼器减小了由于泵的操作而引起的流动通道中的振荡压力的幅度，从而减小了分选过程中实体的速度振荡。 微流体装置可以形成为弹性体材料块，弹性体材料的薄膜可偏转到流动通道中以提供泵或阀功能。 还描述了速度非依赖性细胞计数方法和装置。

公开(公告)号：DE60138218D1

公开(公告)日：2009-05-14

申请号：DE60138218

申请日：2001-11-28

Applicant: CALIFORNIA INST OF TECHN

Inventor： UNGER MARC A ,  CHOU HOU-PU ,  THORSEN TODD A ,  SCHERER AXEL ,  QUAKE STEPHEN R ,  LIU JIAN ,  ADAMS MARK L ,  HANSEN CARL L

IPC: A61F2/00