公开(公告)号：WO1991006631A1

公开(公告)日：1991-05-16

申请号：PCT/US1990006191

申请日：1990-10-26

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： CASE WESTERN RESERVE UNIVERSITY ,  SILVER, Jerry ,  SMITH, George, M. ,  JACOBBERGER, James, W. ,  MILLER, Robert

IPC: C12N05/00

CPC classification number: A61L27/38 ,  A61K35/12 ,  A61K35/30 ,  C12N5/0622 ,  C12N2510/04

Abstract: The present invention relates to ''activated'' astrocytes and the methods of utilizing the activated astrocytes as a means for promoting central nervous system nerve growth and regeneration, blood vessel growth and regeneration, and/or reducing secondary necrosis and glial scar formation. The activated astrocytes and pharmaceutical compositions comprising same, may be used to treat disorders of the nervous system resulting from trauma or diseases which have in some way damaged the nerve tissue.

Abstract translation: 本发明涉及“活化”星形胶质细胞和利用活化星形胶质细胞作为促进中枢神经系统神经生长和再生，血管生长和再生，和/或减少继发性坏死和神经胶质瘢痕形成的手段的方法。 活化的星形胶质细胞和包含其的药物组合物可用于治疗由某种方式损伤神经组织的创伤或疾病引起的神经系统疾病。

公开(公告)号：WO1998019964A1

公开(公告)日：1998-05-14

申请号：PCT/US1997020196

申请日：1997-11-04

Applicant: CASE WESTERN RESERVE UNIVERSITY ,  ANGUS, John, C. ,  ARGOITIA, Alberto ,  HAYMAN, Cliff, C.

Inventor： CASE WESTERN RESERVE UNIVERSITY

IPC: C01B21/064

CPC classification number: C30B15/00 ,  C01B21/0632 ,  C01B21/0641 ,  C01B21/0722 ,  C01P2002/82 ,  C01P2004/03 ,  C01P2004/04 ,  C30B11/00 ,  C30B29/403 ,  C30B29/406 ,  H01L21/02378 ,  H01L21/02387 ,  H01L21/0242 ,  H01L21/0254 ,  H01L21/02614 ,  H01L21/02625

Abstract: A novel method for the synthesis of Group III nitride crystals is disclosed. The method contemplates the reaction of a liquid form of the Group III nitride metal with active nitrogen. The active nitrogen may be generated by a plasma or the thermal decomposition of ammonia and may be neutral, ionic, excited or a mixture thereof. Using the method of the present invention, the crystalline Group III nitride can be grown at pressures of one atmosphere and below. Polycrystalline films grown by this method are of sufficient quality to show luminescence at room temperature.

Abstract translation: 公开了一种合成III族氮化物晶体的新颖方法。 该方法考虑了液态III族氮化物金属与活性氮的反应。 活性氮可以通过等离子体或氨的热分解产生，并且可以是中性的，离子的，激发的或它们的混合物。 使用本发明的方法，可以在一个大气压以下的压力下生长III族氮化物。 通过该方法生长的多晶膜具有足够的质量以在室温下显示发光。

公开(公告)号：WO1998013675A1

公开(公告)日：1998-04-02

申请号：PCT/US1997017067

申请日：1997-09-24

Applicant: CASE WESTERN RESERVE UNIVERSITY ,  GRATZL, Miklos ,  CSEREY, Andrea ,  HUI, Kwong, Yue

Inventor： CASE WESTERN RESERVE UNIVERSITY

IPC: G01J03/00

CPC classification number: B01F13/0272 ,  B01F13/0071 ,  B01F13/0084 ,  B01F13/0093 ,  B01L3/5027 ,  B01L3/5088 ,  B01L2300/0645 ,  B01L2400/0472 ,  G01N21/79 ,  G01N2035/00237 ,  G01N2035/00287 ,  G01N2035/1034

Abstract: A small liquid sample to be tested is contained within an annulus (18) on the upper surface (14) of a substrate (12). A reagent diffuses into the sample through a membrane (28) in a junction hole (26), the junction hole connecting upper (14) and lower (16) surfaces of the substrates. Optical measuring equipment (70, 72) detects a measurable change in an optical property of the sample. Alternatively, flow of gas (30) directed at a small liquid sample (24), contained within an annulus on the surface (14) of a substrate (12), causes the sample to flow in a controlled manner over the surface of an electrode (20), disposed on the substrate surface (14). In another alternative, an electrode (20) is disposed in a non-homogeneous sample (24).

Abstract translation: 要测试的小液体样品被包含在衬底（12）的上表面（14）上的环形空间（18）中。 试剂通过连接孔（26）中的膜（28）扩散到样品中，连接孔连接基板的上部（14）和下部（16）表面。 光学测量设备（70,72）检测样品的光学特性的可测量的变化。 或者，包含在衬底（12）的表面（14）上的环形空间内的指向小液体样品（24）的气体（30）的流动使得样品以受控的方式流过电极的表面 （20），设置在所述基板表面（14）上。 在另一替代方案中，电极（20）设置在非均质样品（24）中。

公开(公告)号：WO1998012316A1

公开(公告)日：1998-03-26

申请号：PCT/US1997016774

申请日：1997-09-22

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： CASE WESTERN RESERVE UNIVERSITY ,  HARRINGTON, John, J. ,  VAN BOKKELEN, Gil, B. ,  WILLARD, Huntington, F.

IPC: C12N15/10

CPC classification number: C12N15/85 ,  C12N15/66 ,  C12N15/90 ,  C12N2800/204

Abstract: A direction method of stably cloning large arrays of repetitive DNA is described. The method is especially useful for the stable cloning of alpha satellite DNA.

Abstract translation: 描述了稳定克隆大量重复DNA的方向方法。 该方法对于α卫星DNA的稳定克隆特别有用。

公开(公告)号：WO1997044481A1

公开(公告)日：1997-11-27

申请号：PCT/US1997008703

申请日：1997-05-21

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： CASE WESTERN RESERVE UNIVERSITY ,  DE BOER, Piet, A.J. ,  HALE, Cynthia, A.

IPC: C12Q01/25

CPC classification number: C07K14/245 ,  G01N33/9446 ,  Y10S530/81 ,  Y10S530/811 ,  Y10S530/814 ,  Y10S530/817 ,  Y10S530/825 ,  Y10S930/28

Abstract: A method for screening compounds for antimicrobial activity is described that utilizes bacterial protein-protein binding in vitro. The method may be performed using immobilized elements and the immobilization may be carried out using a variety of immobilization means e.g., columns, beads, adsorbents, nitrocellulose paper, etc.) in order to screen large libraries of compounds.

Abstract translation: 描述了筛选化合物用于抗微生物活性的方法，其在体外利用细菌蛋白质 - 蛋白质结合。 该方法可以使用固定化元件进行，并且可以使用各种固定装置例如柱，珠，吸附剂，硝化纤维素纸等进行固定），以筛选大量化合物文库。

公开(公告)号：WO1997041208A1

公开(公告)日：1997-11-06

申请号：PCT/US1997006760

申请日：1997-04-24

Applicant: CASE WESTERN RESERVE UNIVERSITY ,  SORRELL, J., Michael ,  CAPLAN, Arnold, I.

Inventor： CASE WESTERN RESERVE UNIVERSITY

IPC: C12N05/00

CPC classification number: A61L27/3891 ,  A61K35/12 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0656 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2502/1358 ,  C12N2533/54

Abstract: Disclosed is a multilayer skin equivalent having (i) a scaffold layer incorporated with dermis-forming cells, and (ii) a keratinocyte layer. Also disclosed is a multilayer dermal equivalent having at least one of (i) a first layer of at least one skin-associated extracellular matrix component containing papillary dermal fibroblasts; and, in laminar relationship therewith, (ii) a second layer of at least one skin-associated extracellular matrix component containing reticular dermal fibroblasts. Preferred embodiments include those where the scaffold or dermis-forming layer is a multilayer selected from the group consisting of (a) a layer containing isolated papillary dermis-forming cells and a layer containing isolated reticular dermis-forming cells; (b) a layer containing isolated papillary dermis-forming cells and a layer containing isolated, culture expanded mesenchymal stem cells; and (c) a layer containing isolated reticular dermis-forming cells and a layer containing isolated, culture expanded mesenchymal stem cells. Also enclosed is a multilayer skin equivalent having (i) a scaffold layer comprising a first layer of at least one skin-associated extracellular matrix component containing papillary dermal fibroblasts and, in laminar relationship therewith, a second layer of at least one skin-associated extracellular matrix component containing reticular dermal fibroblasts; and (ii) a keratinocyte layer. Also disclosed is an injectable composition of dermis-forming cells and at least one skin-associated extracellular matrix component in a pharmaceutically acceptable injectable carrier. The injectable composition can further include keratinocytes.

Abstract translation: 公开了一种多层皮肤当量，其具有（i）与真皮形成细胞结合的支架层，和（ii）角质形成细胞层。 还公开了具有以下至少一种的多层皮肤当量：（i）含有乳头状真皮成纤维细胞的至少一种皮肤相关细胞外基质组分的第一层; 并且与其层状关系，（ii）含有网状真皮成纤维细胞的至少一种皮肤相关的细胞外基质组分的第二层。 优选的实施方案包括其中支架或真皮形成层是选自（a）含有分离的乳头状真皮细胞形成细胞层和含有分离的网状真皮形成细胞的层的多层的那些实例; （b）含有分离的乳头状真皮细胞的层和含有分离培养的扩展的间充质干细胞的层; 和（c）含有分离的网状真皮细胞的层和含有分离的培养扩展的间充质干细胞的层。 还包括多层皮肤等效物，其具有（i）支架层，其包含含有乳头状真皮成纤维细胞的至少一种皮肤相关细胞外基质组分的第一层，并且与其层叠，具有至少一种皮肤相关细胞外的第二层 含有网状真皮成纤维细胞的基质成分; 和（ii）角质形成细胞层。 还公开了在药学上可接受的可注射载体中的真皮形成细胞和至少一种与皮肤相关的细胞外基质组分的可注射组合物。 可注射组合物还可包含角质形成细胞。

公开(公告)号：WO1997035477A1

公开(公告)日：1997-10-02

申请号：PCT/US1997004917

申请日：1997-03-25

Applicant: THE PENN STATE RESEARCH FOUNDATION ,  CASE WESTERN RESERVE UNIVERSITY ,  PEGG, Anthony, E. ,  GERSON, Stanton, L.

Inventor： THE PENN STATE RESEARCH FOUNDATION ,  CASE WESTERN RESERVE UNIVERSITY

IPC: A01N43/04

CPC classification number: C12N9/1085 ,  A61K38/45 ,  A61K48/00 ,  A61K2300/00

Abstract: The present invention relates to methods of treating neoplastic disease whereby gene therapy treatments are employed in combination with a chemotherapy regime. A combinational therapy with anti-neoplastic alkylating agents will optimize host tumor sensitivity to these agents used alone or in combination with O -benzylguanine (BG) or a similar compound or compounds. Hematopoietic cells are infected with a transgene expressing a mutant AGT protein exhibiting DNA repair activity while imparting resistance to BG or a related compound. Introduction of the transduced hematopoietic cell population expressing the mutant AGT protein into the patient in tandem with the chemotherapeutic regime will substantially reduce myelosuppression traditionally associated with the administration of these anti-neoplastic drugs.

Abstract translation: 本发明涉及治疗肿瘤性疾病的方法，其中基因疗法治疗与化疗方案结合使用。 具有抗肿瘤烷化剂的组合疗法将使单独使用或与O 6 - 苄基鸟嘌呤（BG）或类似化合物或组合物组合使用的这些药物的宿主肿瘤敏感性优化。 造血细胞用表达具有DNA修复活性的突变型AGT蛋白的转基因感染，同时赋予对BG或相关化合物的抗性。 与化疗方案一起将表达突变型AGT蛋白的转导的造血细胞群导入患者将显着降低传统上与施用这些抗肿瘤药物相关的骨髓抑制。

公开(公告)号：WO1997018243A1

公开(公告)日：1997-05-22

申请号：PCT/US1996018493

申请日：1996-11-13

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： CASE WESTERN RESERVE UNIVERSITY ,  MARCHANT, Roger, E. ,  ZHANG, Tianhong

IPC: C08B37/00

CPC classification number: C07H15/12 ,  C08B37/0021 ,  C11D1/525 ,  C11D1/662

Abstract: The present invention provides new saccharide surfactants. The saccharide surfactants include diblock and triblock saccharide surfactants. The diblock saccharide surfactants have a hydrophobic segment bonded to a hydrophilic head group, that is a saccharide chain. The triblock saccharide surfactants have a hydrophobic segment bonded to two hydrophilic saccharide chains. The hydrophobic segments of the diblick and triblock saccharide surfactants comprise at least one alkyl chain having 5 to 20 carbon atoms, preferably 6 to 18 carbon atoms, more preferably 8 to 18 carbon atoms. The saccharide head groups/chains include monosaccharides, disaccharides, and oligosaccharides and have an average weight molecular range of less than 4000. Preferably the saccharide head group/chain comprises 2 to 12 sugar residues. Preferably the saccharide head groups are linked to the alkyl chains by an amide group.

Abstract translation: 本发明提供新的糖表面活性剂。 糖表面活性剂包括二嵌段和三嵌段糖表面活性剂。 二嵌段糖表面活性剂具有与亲水性头基键合的疏水链段，即糖链。 三嵌段糖表面活性剂具有与两个亲水性糖链结合的疏水链段。 二元和三嵌段糖表面活性剂的疏水链段包含至少一个具有5至20个碳原子，优选6至18个碳原子，更优选8至18个碳原子的烷基链。 糖头组/链包括单糖，二糖和寡糖，并且具有小于4000的平均重量分子范围。优选地，糖头基/链包含2至12个糖残基。 优选地，糖头基团通过酰胺基团与烷基链连接。

公开(公告)号：WO1996024848A1

公开(公告)日：1996-08-15

申请号：PCT/US1996000370

申请日：1996-01-03

Applicant: CASE WESTERN RESERVE UNIVERSITY ,  BRUDER, Scott, P. ,  CAPLAN, Arnold, I. ,  HAYNESWORTH, Stephen, E.

Inventor： CASE WESTERN RESERVE UNIVERSITY

IPC: G01N33/567

CPC classification number: C07K16/28

Abstract: Monoclonal antibodies specific for normal human osteogenic cell subsets and the monoclonal hybridoma cell lines that synthesize and secrete these monospecific antibodies, as well as the uses of the monoclonal antibodies for diagnostic and/or therapeutic purposes.

Abstract translation: 对正常人成骨细胞亚群特异性的单克隆抗体以及合成和分泌这些单特异性抗体的单克隆杂交瘤细胞系以及用于诊断和/或治疗目的的单克隆抗体的用途。

公开(公告)号：WO1996016418A1

公开(公告)日：1996-05-30

申请号：PCT/US1995014770

申请日：1995-11-09

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： CASE WESTERN RESERVE UNIVERSITY ,  KO, Wen, H.

IPC: H01G07/00

CPC classification number: G01L9/0073

Abstract: A capacitive absolute pressure sensor (10) includes a substrate (14) having an electrode (24) deposited thereon and a diaphragm assembly (12) disposed on the substrate (14). As pressure increases, the diaphragm (16) deflects, touches the electrode (24) (in the touch mode), and changes the capacitance of the sensor (10). The changed capacitance is sensed to thus sense pressure changes. A burried feedthrough is used to sense the change in a capacitance in a chamber (34) under the diaphragm (16) and thus determine the pressure sensed. A vacuum in the chamber (34) is maintained by proper selection of a thickness of a sensing electrode and an insulating layer (32), exposition thereof to a thermal cycle, and the hermetic bonding of the diaphragm assembly (12) to the substrate (14).

Abstract translation: 电容绝对压力传感器（10）包括其上沉积有电极（24）的基板（14）和设置在基板（14）上的隔膜组件（12）。 随着压力的增加，隔膜（16）偏转，触摸电极（24）（触摸模式），并改变传感器（10）的电容。 感测到改变的电容，从而感测压力变化。 使用埋葬馈通来感测隔膜（16）下方的室（34）中的电容的变化，从而确定所感测的压力。 通过适当选择感测电极和绝缘层（32）的厚度，将其暴露于热循环，以及将隔膜组件（12）与衬底（12）的气密接合保持在室（34）中的真空 14）。