公开(公告)号：US20130338968A1

公开(公告)日：2013-12-19

申请号：US13969830

申请日：2013-08-19

Applicant: OLYMPUS CORPORATION

Inventor： Takuya Hanashi ,  Mitsushiro Yamaguchi ,  Tetsuya Tanabe

IPC: G01N15/14

CPC classification number: G01N15/1429 ,  G01N21/51 ,  G01N21/6408 ,  G01N21/645 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0084

Abstract: There is provided a structure to make the setting of a criterion for eliminating noises easy in the scanning molecule counting method. In the inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution, time series light intensity data of light from a light detection region detected with moving the position of the light detection region in the sample solution is generated, and a signal of a light-emitting particle individually is detected in the time series light intensity data, wherein a signal having a light intensity in a light intensity range set based upon a signal generation frequency integrated value distribution which is a distribution, obtained by using as a variable an intensity of a signal, of integrated values of generation frequencies of signals having an intensity not lower than the variable is extracted as the signal of the light-emitting particle.

Abstract translation: 提供了一种结构，用于设置扫描分子计数方法中消除噪声的标准。 在本发明的用于检测样品溶液中的发光粒子的光的光学分析技术中，产生了通过移动样品溶液中的光检测区域的位置而检测到的光检测区域的光的时间序列光强度数据， 在时间序列光强度数据中检测出发光粒子的信号，其中，根据作为分配获得的作为分布的信号生成频率积分值分布设定的光强度的光强度的信号， 提取具有不低于该变量的信号的信号的产生频率的积分值的信号强度的变量作为发光粒子的信号。

公开(公告)号：US20130302906A1

公开(公告)日：2013-11-14

申请号：US13946091

申请日：2013-07-19

Applicant: OLYMPUS CORPORATION

Inventor： Tetsuya Tanabe

IPC: G01J1/42

CPC classification number: G01J1/42 ,  G01N21/6452 ,  G02B21/0076 ,  G02B21/16

Abstract: In the scanning molecule counting method using the light measurement with a confocal microscope or a multiphoton microscope, a measuring time is optimized with suppressing the scattering in a result small irrespective of light-emitting particle concentrations. In the inventive technique of detecting and analyzing the light from an light-emitting particle, there are repeated processes of detecting the light intensity from a light detection region with moving the position of the light detection region of an optical system in a sample solution by changing the optical path of the optical system of the microscope, and detecting the signals of the light of light-emitting particles individually, and based on the time taken for the number of the signals from the light-emitting particles to reach a predetermined number, the light-emitting particle concentration in the sample solution is determined.

Abstract translation: 在使用共聚焦显微镜或多光子显微镜的光测量的扫描分子计数方法中，不考虑发光粒子浓度，抑制结果中的散射小而优化测定时间。 在本发明的检测和分析来自发光粒子的光的技术中，通过改变样品溶液中的光学系统的光检测区域的位置来检测来自光检测区域的光强度的重复处理 显微镜的光学系统的光路，并且分别检测发光粒子的光的信号，并且基于从发光粒子的信号数达到预定数目所花费的时间， 确定样品溶液中的发光粒子浓度。

公开(公告)号：US20130230874A1

公开(公告)日：2013-09-05

申请号：US13789111

申请日：2013-03-07

Applicant: OLYMPUS CORPORATION

Inventor： Takuya Hanashi ,  Tetsuya Tanabe ,  Mitsushiro Yamaguchi ,  Hidetaka Nakata

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458

Abstract: There is provided an optical analysis technique enabling identification of a kind of light-emitting particle corresponding to a signal on a time series light intensity data or identification of a signal corresponding to light-emitting particles other than a particle to be observed in an optical measurement using a confocal microscope or a multiphoton microscope. The inventive optical analysis technique measures simultaneously and separately intensities of lights of two or more wavelength bands from a light detection region in a sample solution containing light-emitting particles of two or more kinds to generate time series light intensity data of the respective wavelength bands; detects signals simultaneously generated on the time series light intensity data of at least two wavelength bands; and identifies the simultaneously generated signals as signals of a light-emitting particle of at least one specific kind.

Abstract translation: 提供了一种光学分析技术，其能够对与光学测量中要观察的颗粒以外的发光粒子相对应的时间序列光强度数据或对应于发光粒子的信号的识别相对应的一种发光粒子 使用共焦显微镜或多光子显微镜。 本发明的光学分析技术测量来自含有两种或更多种发光粒子的样品溶液中的光检测区域的两个或更多个波长带的光的同时且分开的强度，以产生各波长带的时间序列光强度数据; 检测同时产生的至少两个波长带的时间序列光强数据的信号; 并将同时生成的信号识别为至少一种特定种类的发光粒子的信号。

公开(公告)号：US20130207007A1

公开(公告)日：2013-08-15

申请号：US13840122

申请日：2013-03-15

Applicant: OLYMPUS CORPORATION

Inventor： Tetsuya Tanabe

IPC: G01N21/85

CPC classification number: G01N21/85 ,  G01N15/1434 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/1087 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/008

Abstract: There is provided a method of avoiding deterioration of the accuracy in the number of detected light-emitting particles due to that two or more light-emitting particles are encompassed at a time in the light detection region in the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope. In the inventive optical analysis technique, in the detection of an individual signal indicating light of a light-emitting particle by selectively detecting a signal having an intensity beyond a threshold value as a signal indicating light of a light-emitting particle in light intensity data produced through measuring light intensity during moving the position of a light detection region in a sample solution, the threshold value is set so that a signal indicating light from a light-emitting particle encompassed in a region narrower than the light detection region will be detected selectively.

Abstract translation: 提供了一种避免由于在使用光学测量的扫描分子计数方法中在光检测区域中一次包围两个或更多个发光粒子而使检测到的发光粒子数量的精度降低的方法 用共焦显微镜或多光子显微镜。 在本发明的光学分析技术中，在通过选择性地检测具有超过阈值的强度的信号来检测指示发光粒子的光的单个信号作为产生光强度数据中的发光粒子的光的信号 通过在移动样品溶液中的光检测区域的位置时测量光强度，设定阈值，以便选择性地检测表示来自比光检测区域窄的区域中的发光粒子的光的信号。