Abstract:
A separation chip for separating an insoluble component from a suspension using centrifugal force by rotation, includes a suspension holding tank, a separation liquid holding tank, and an insoluble component holding tank which are arranged in this order from an inner circumferential side during rotation. In the separation chip, the suspension holding tank and the insoluble component holding tank are connected with each other, the insoluble component holding tank and the separation liquid holding tank are connected with each other by a narrow portion, and in the insoluble component holding tank, a connecting portion with the suspension holding tank is positioned further toward an outer circumferential side than the narrow portion.
Abstract:
A liquid-feeding chip for feeding a liquid utilizing the action of centrifugal force and gravity by rotating the chip around an axis of rotation, includes a first storage tank (1-1) into which the liquid can be introduced when rotation of the chip is stopped, and two or more liquid-feeding units arranged in a plurality of levels adjacent to each other, each liquid-feeding unit (U-1, U-2, U-3) being composed of a first holding tank (10-1, 20-1, 30-1), a second holding tank (10-2, 20-2, 30-2) positioned in the direction of gravity with respect to the first holding tank, and a channel B (B-1, B-2, B-3) which extends from the first holding tank in the direction of gravity and which connects the first holding tank and the second holding tank, the first holding tank at a first level being connected with a channel A (A-1) which extends from the first storage tank toward an outer circumferential side. In the liquid-feeding chip, the adjacent liquid-feeding units are connected by a channel C (C-1, C-2) which extends from the second holding tank of the liquid-feeding unit at an upper level to an outer circumferential side during rotation, and which is in communication with the first holding tank of the liquid-feeding unit at a lower level.
Abstract:
[PROBLEMS] To provide a method for producing a chemical product with a high yield using a mixed sugar of hexose and pentose as a fermentation feedstock. [MEANS FOR SOLUTION] A method for producing a chemical product by continuous fermentation, which method comprises filtering a culture liquid of a microorganism(s) through a separation membrane; retaining unfiltered liquid in, or refluxing unfiltered liquid to, the culture liquid; adding a fermentation feedstock to the culture liquid; and recovering a product in the filtrate, wherein the fermentation feedstock contains pentose and hexose, and wherein the microorganism(s) is/are a microorganism(s) having a pathway in which pentose reductase and pentol dehydrogenase are used to metabolize pentose, was provided.
Abstract:
By using a transgenic silkworm having a gene, which encodes a spider thread protein having desired properties (a high strength, a high elongation, etc.), transferred thereinto without injuring silkworm fibroin H-chain gene by a means of, for example, using the transposon function, the spider thread protein having the desired properties is produced by the transgenic silkworm without lowering the strength or elongation of silk thread produced by the transgenic silkworm, thereby providing a hybrid silk of spider thread with silk thread having the desired properties.
Abstract:
It is intended to provide a genetic engineering material for insects whereby a target protein can be easily purified without resort to a recombinant baculovirus and a process for producing a foreign protein with the use of this genetic engineering material. A genetically modified silk worm is obtained by transferring a foreign protein gene (for example, a cytokine gene) ligated to a promoter functioning in the silk gland into silkworm chromosome. Then the foreign protein (for example, cytokine) is extracted and purified from the silk gland or cocoon of the silkworm or its offspring. By transferring a gene cassette for expression wherein the 5’-terminal DNA sequence of fibroin H-chain gene is fused with the 3’-terminal DNA sequence of a foreign protein gene into silk gland cells or the like, the foreign protein can be produced in a large amount both inside and outside the silk gland cells as well as in silk yarn and cocoon.