公开(公告)号：ES2544710T3

公开(公告)日：2015-09-03

申请号：ES11750425

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  WATANABE SHIOMI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N9/42 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12P19/02

Abstract: Procedimiento para producir una β-glucosidasa mutante derivada de un termófilo que tiene fijada selectivamente a ella una cadena de azúcares y también tiene una actividad glucosidasa, que comprende: (i) preparar ADN que codifica una β-glucosidasa mutante derivada de un termófilo introduciendo una secuencia de ADN que codifica Asn-X-Ser o Asn-X-Thr (en las que, X es cualquier aminoácido excepto prolina) en ADN que codifica una β-glucosidasa derivada de un termófilo que está originalmente desprovista de una secuencia de glucosilación y, además, añadiendo una secuencia de ADN que codifica una secuencia señal de secreción al ADN que codifica una β-glucosidasa mutante, (ii) introducir el ADN que codifica una β-glucosidasa mutante al que se le ha añadido la secuencia de ADN que codifica la secuencia señal de secreción en un microorganismo eucariota, de modo que una β-glucosidasa mutante codificada por el ADN de la β-glucosidasa mutante se exprese como una proteína secretora, y (iii) aislar y purificar la β-glucosidasa mutante expresada de este modo como una proteína secretora.

公开(公告)号：AU2013208441A1

公开(公告)日：2014-08-21

申请号：AU2013208441

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： ISOBE KYOHEI ,  WATANABE SHIOMI ,  KOBAYASHI KOJI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/06 ,  C12P7/18 ,  C12P7/56

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the fermentation raw material comprises a pentose and a hexose and the microorganism has a pathway through which a pentose can be metabolized with a pentose isomerase.

公开(公告)号：AU2013208440B2

公开(公告)日：2016-12-01

申请号：AU2013208440

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： WATANABE SHIOMI ,  KOBAYASHI KOJI ,  ISOBE KYOHEI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/06 ,  C12P7/56

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the fermentation raw material comprises a pentose and a hexose and the microorganism has a pathway through which a pentose can be metabolized with a pentose reductase and a pentol dehydrogenase.

公开(公告)号：AU2013208440A1

公开(公告)日：2014-08-21

申请号：AU2013208440

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： WATANABE SHIOMI ,  KOBAYASHI KOJI ,  ISOBE KYOHEI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/06 ,  C12P7/56

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the fermentation raw material comprises a pentose and a hexose and the microorganism has a pathway through which a pentose can be metabolized with a pentose reductase and a pentol dehydrogenase.

公开(公告)号：CA2860759A1

公开(公告)日：2013-07-18

申请号：CA2860759

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： WATANABE SHIOMI ,  KOBAYASHI KOJI ,  ISOBE KYOHEI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/06 ,  C12P7/56

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the fermentation raw material comprises a pentose and a hexose and the microorganism has a pathway through which a pentose can be metabolized with a pentose reductase and a pentol dehydrogenase.

公开(公告)号：CA2796363A1

公开(公告)日：2011-10-20

申请号：CA2796363

申请日：2011-04-11

Applicant: TORAY INDUSTRIES

Inventor： SAWAI KENJI ,  WATANABE SHIOMI ,  MIMITSUKA TAKASHI ,  SAWAI HIDEKI

IPC: C12P13/00 ,  C07C211/09 ,  C12N9/88 ,  C12N15/09

Abstract: Disclosed is a method for producing 1,5-pentanediamine through fermentation using a microorganism, said microorganism having LDC gene in the chromosome and showing regulated secondary production of L-lysine. In the method for producing 1,5-pentanediamine whereby 1,5-pentanediamine is produced by a coryneform bacterium having a gene encoding lysine decarboxylase in the chromosome, said coryneform bacterium continuously maintains a lysine decarboxylase activity of 50 mU/mg protein or greater during the culture.

公开(公告)号：BR112012021230A2

公开(公告)日：2015-10-27

申请号：BR112012021230

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WATANABE SHIOMI ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N1/19 ,  C12N1/20 ,  C12N9/42 ,  C12N15/09 ,  C12P19/02

公开(公告)号：CA2860761A1

公开(公告)日：2013-07-18

申请号：CA2860761

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： ISOBE KYOHEI ,  WATANABE SHIOMI ,  KOBAYASHI KOJI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/06 ,  C12P7/18 ,  C12P7/56

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the fermentation raw material comprises a pentose and a hexose and the microorganism has a pathway through which a pentose can be metabolized with a pentose isomerase.

公开(公告)号：CA2860756A1

公开(公告)日：2013-07-18

申请号：CA2860756

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： KOBAYASHI KOJI ,  WATANABE SHIOMI ,  ISOBE KYOHEI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/08 ,  C12P19/14

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the microorganism can undergo catabolite inhibition and the fermentation raw material comprises a hexose and a pentose.

公开(公告)号：AU2011222255A1

公开(公告)日：2012-10-11

申请号：AU2011222255

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  WATANABE SHIOMI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N9/42 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12P19/02

Abstract: Provided is an efficient method for decomposing biomass, which involves producing glucosidase in which the cellulose degrading efficiency is improved, and using said glucosidase. Specifically, provided is a method for producing a mutant glucosidase wherein a DNA encoding a secretion signal sequence and a DNA encoding Asn-X-Ser or Asn-X-Thr are introduced into a DNA encoding a glucosidase from a thermophile, and the resulting product is introduced into a eukaryotic microorganism and expressed as a secretory protein. Also provided are an enzyme composition containing said mutant glucosidase, and a biomass hydrolysis method using said enzyme composition.