公开(公告)号：US4936682A

公开(公告)日：1990-06-26

申请号：US084098

申请日：1987-08-11

Applicant: Joshua K. Hoyt

Inventor： Joshua K. Hoyt

IPC: G01N35/02 ,  G01N21/25 ,  G01N21/27

CPC classification number: G01N21/253 ,  G01N2201/0407 ,  G01N2201/0415 ,  G01N2201/0476

Abstract: An analytic instrument for kinetically measuring light absorption characteristics of a plurality of independent samples contained in disposable test tubes arranged in a circular pattern about a single light source. The instrument is designed to be used with a host personal computer and is not specific to any particular type of assay. The instrument obtains and temporarily stores raw data in the form of digitized output signals from the plurality of photodetectors and periodically passes them to the host computer. An incubator has test tube wells arranged in a circular array equidistant from a single incandescent light source. The circular symmetry controls the thermal gradients in the incubator such that all of the samples are disposed on the same isotherm and all of the photodetectors are disposed on the same isotherm. The instrument is designed for use with disposable test tubes, and sources of error arising from optical variation in such test tubes are minimized by providing a separate detector for each tube and by holding the tube fixed with respect to the detector. The light output from the single light source is continuously monitored and is kept constant.

公开(公告)号：US4738534A

公开(公告)日：1988-04-19

申请号：US30931

申请日：1987-03-26

Applicant: Kenneth R. Houseman ,  David C. Wender ,  Lawrence G. Banovez ,  Mieczyslaw Wroblewski

Inventor： Kenneth R. Houseman ,  David C. Wender ,  Lawrence G. Banovez ,  Mieczyslaw Wroblewski

IPC: G01N21/27 ,  G01J3/12 ,  G01N21/25 ,  G01N21/03 ,  G01N21/31

CPC classification number: G01N21/253 ,  G01J2003/123 ,  G01N2201/0407 ,  G01N2201/0826 ,  Y10S436/807

Abstract: A vertical beam spectrophotometer for measuring the light absorption of an assay prepared using standard wet chemistry procedures and conventional solid phase coated bead technology is disclosed. The spectrophotometer measures the absorption of the assay in a conventional reaction cuvette with the bead remaining in the cuvette. The light source of the spectrophotometer illuminates the bead, which diffuses the light into the surrounding assay solution. A lense projects the diffused light onto a photocell which converts it into an electrical signal having magnitude related to the light absorption of the assay. The signal is processed in a known manner by conventional processing circuitry to obtain an absorption value.

公开(公告)号：US09829434B2

公开(公告)日：2017-11-28

申请号：US15070211

申请日：2016-03-15

Applicant: Bio-Rad Laboratories Inc.

Inventor： Yochanan Uri ,  Boaz Ran ,  Ariel G. Notcovich ,  Shay Nimri

IPC: G01N21/01 ,  G01N21/65 ,  G01N21/25 ,  G01N21/552 ,  G01N33/543 ,  G01N21/03

CPC classification number: G01N21/553 ,  G01N21/253 ,  G01N21/658 ,  G01N33/54373 ,  G01N2021/0328 ,  G01N2201/0407 ,  G01N2201/0484 ,  G01N2201/062

Abstract: A tip for use in an optical detection system to analyze an analyte in a fluid sample drawn into the tip, using light reflected from a detection surface inside the tip that the analyte binds to, comprising a first detection surface and a second detection surface located in a same flow path with no controllable valve separating them, wherein the first and second detection surfaces have different surface chemistries.

公开(公告)号：US20150238919A1

公开(公告)日：2015-08-27

申请号：US14610814

申请日：2015-01-30

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Mark F. OLDHAM ,  Dar BAHATT ,  Kenneth J. LIVAK ,  Jason E. BABCOKE ,  H. Pin KAO ,  Stephen J. GUNSTREAM ,  Kevin S. BODNER ,  Douglas P. GREINER ,  Nigel P. BEARD

IPC: B01J19/00 ,  G01N21/17 ,  B01L7/00

CPC classification number: B01J19/0046 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/185 ,  B01L2400/0655 ,  G01N21/17 ,  G01N2201/0407

Abstract: Exemplary embodiments provide microfluidic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.

Abstract translation: 示例性实施例提供了用于其使用的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。

公开(公告)号：US08945481B1

公开(公告)日：2015-02-03

申请号：US12145333

申请日：2008-06-24

Applicant: Mark F. Oldham ,  Kenneth J. Livak ,  Jason E. Babcoke ,  H. Pin Kao ,  Stephen J. Gunstream ,  Kevin S. Bodner ,  Douglas P. Greiner ,  Nigel P. Beard ,  Dar Bahatt

Inventor： Mark F. Oldham ,  Kenneth J. Livak ,  Jason E. Babcoke ,  H. Pin Kao ,  Stephen J. Gunstream ,  Kevin S. Bodner ,  Douglas P. Greiner ,  Nigel P. Beard ,  Dar Bahatt

IPC: B01L3/00

CPC classification number: B01J19/0046 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/185 ,  B01L2400/0655 ,  G01N21/17 ,  G01N2201/0407

Abstract: Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.

Abstract translation: 示例性实施例提供了用于它们的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。

公开(公告)号：US20110076199A1

公开(公告)日：2011-03-31

申请号：US12876405

申请日：2010-09-07

Applicant: Paul Meller ,  Holger Pufahl

Inventor： Paul Meller ,  Holger Pufahl

IPC: G01N21/59 ,  G01N33/48

CPC classification number: G01N21/253 ,  G01N21/0332 ,  G01N21/255 ,  G01N35/02 ,  G01N2021/0325 ,  G01N2021/825 ,  G01N2035/00534 ,  G01N2201/0407 ,  G01N2201/0423 ,  G01N2201/0453 ,  G01N2201/0627

Abstract: A device for the photometric examination of samples has a sample-holder apparatus for at least two sample vessels, and a measuring apparatus and a moveable apparatus. The sample-holder apparatus is designed to be stationary, and the measuring apparatus is arranged on the moveable apparatus such that it can be displaced by means of the moveable apparatus.

Abstract translation: 用于样品的光度检查的装置具有用于至少两个样品容器的样品保持器装置，以及测量装置和可移动装置。 样品架设备被设计为静止的，并且测量设备被布置在可移动设备上，使得其可以借助于可移动设备而移位。

公开(公告)号：US20090174936A1

公开(公告)日：2009-07-09

申请号：US12365779

申请日：2009-02-04

Applicant: ARTUR G. OLSZAK

Inventor： ARTUR G. OLSZAK

IPC: G02B21/18

CPC classification number: G02B21/002 ,  G01N21/253 ,  G01N2201/0407

Abstract: A microscope array for simultaneously imaging multiple objects. A preferred embodiment of a method according to the invention includes arranging the objects into an array, providing a microscope array having a plurality of imaging elements with respective fields of view arranged into a corresponding array such that the imaging elements are optically aligned respectively with the objects, and simultaneously imaging the objects with the microscope array to produce respective images of the objects. The invention also provides for scanning while imaging, and for stepping and repeating the imaging process.

Abstract translation: 用于同时成像多个物体的显微镜阵列。 根据本发明的方法的优选实施例包括将物体布置成阵列，提供具有多个成像元件的显微镜阵列，其具有排列成相应阵列的各个视场，使得成像元件分别与物体 并且同时用显微镜阵列成像物体以产生物体的各个图像。 本发明还提供了成像时的扫描，以及步进和重复成像过程。

公开(公告)号：US20070206192A1

公开(公告)日：2007-09-06

申请号：US11363938

申请日：2006-03-01

Applicant: Pavel Fomitchov ,  Eugene Barash ,  Ahmad Yekta ,  Joseph Masino

Inventor： Pavel Fomitchov ,  Eugene Barash ,  Ahmad Yekta ,  Joseph Masino

IPC: G01N21/25

CPC classification number: G01N21/6458 ,  G01N21/47 ,  G01N2201/0407 ,  G01N2201/1042 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/367

Abstract: A system and method for multimode imaging of at least one sample is disclosed. The system includes at least one light source; an optical system selected responsive to a mode of operation of the imaging system; and a detector capable of selective reading of pixels. The at least one sample is moved elative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving. The method includes the steps of (1) selecting a mode of operation for the imaging system; (2) transmitting light from at least one light source through an optical system selected in response to the mode of operation for the imaging system; (3) moving the at least one sample relative to the optical system using a sample movement technique selected from the group consisting of step sample moving and continuous sample moving; and (4) selectively reading pixels with a detector.

Abstract translation: 公开了一种用于至少一个样品的多模成像的系统和方法。 该系统包括至少一个光源; 响应于成像系统的操作模式选择的光学系统; 以及能够选择性地读取像素的检测器。 使用选自步骤样品移动和连续样品移动的样品移动技术将至少一个样品移动到光学系统。 该方法包括以下步骤：（1）选择成像系统的操作模式; （2）从至少一个光源透射通过响应于成像系统的操作模式选择的光学系统的光; （3）使用选自步骤样品移动和连续样品移动的样本移动技术来移动所述至少一个样品相对于所述光学系统; 和（4）用检测器选择性地读取像素。

公开(公告)号：US20070009383A1

公开(公告)日：2007-01-11

申请号：US11174957

申请日：2005-07-05

Applicant: William Bedingham ,  Peter Ludowise ,  Barry Robole

Inventor： William Bedingham ,  Peter Ludowise ,  Barry Robole

IPC: G01N35/00

CPC classification number: F16K99/003 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/025 ,  B01L2300/021 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/004 ,  F16K2099/0084 ,  G01N21/0332 ,  G01N21/07 ,  G01N21/359 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/00069 ,  G01N35/1097 ,  G01N2021/0325 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2035/00267 ,  G01N2035/00415 ,  G01N2035/0474 ,  G01N2201/0407 ,  G01N2201/0415 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/0833 ,  Y10T436/11 ,  Y10T436/111666

Abstract: Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Abstract translation: 描述了用于在实时PCR（聚合酶链式反应）中检测多种靶物种的技术。 例如，系统包括数据采集装置和耦合到数据采集装置的检测装置。 检测装置包括具有多个处理室的旋转盘，该多个处理室具有发射不同波长的荧光的多种物质。 该装置还包括多个可移除的光学模块，其被光学配置为激发物种并捕获由不同波长的物质发射的荧光。 耦合到多个可移除光学模块的光纤束将来自光学模块的荧光传送到单个检测器。 此外，该装置可以通过利用激光加热阀来定位和选择性地打开阀分离腔来控制盘中的流体流动。

公开(公告)号：US20060202133A1

公开(公告)日：2006-09-14

申请号：US11345262

申请日：2006-02-01

Applicant: Gyeong-sik Ok ,  Su-hyeon Kim ,  Jin-tae Kim ,  Kwang-wook Oh

Inventor： Gyeong-sik Ok ,  Su-hyeon Kim ,  Jin-tae Kim ,  Kwang-wook Oh

IPC: G01J1/58 ,  F21V9/16

CPC classification number: G01J3/10 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/0346 ,  G01N2021/3174 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484 ,  G01N2201/0221 ,  G01N2201/0407 ,  G01N2201/0461 ,  G01N2201/0612 ,  G01N2201/0627 ,  G01N2201/0631 ,  G01N2201/0806 ,  G01N2201/0833

Abstract: A multi-channel fluorescence measuring optical system and a multi-channel fluorescence sample analyzer using the optical system are provided. The multi-channel fluorescence measuring optical system, which irradiates light onto a plurality of sample channels and detecting fluorescence radiated from samples, includes: a light source; an integrator for giving the light irradiated from the light source a uniform intensity distribution; a sample holder having a plurality of sample channels on which the samples are mounted, wherein the samples are exited by the light emitted from the integrator; and a beam splitter between the integrator and the sample holder for dividing the incident light in a predetermined ratio. Since the light intensities of fluorescence images are detected using optical fiber bundles and photodiodes, the manufacturing cost can be greatly reduced, and the optical system can be miniaturized.

Abstract translation: 提供多通道荧光测量光学系统和使用该光学系统的多通道荧光样品分析仪。 多通道荧光测量光学系统，其将光照射到多个样品通道上并检测从样品辐射的荧光，包括：光源; 用于给从光源照射的光均匀的强度分布的积分器; 具有多个样品通道的样品架，样品安装在样品通道上，其中采样由积分器发出的光退出; 以及在积分器和样品保持器之间的分束器，用于以预定比例分割入射光。 由于使用光纤束和光电二极管检测荧光图像的光强度，因此可以大大降低制造成本，并且可以使光学系统小型化。