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公开(公告)号：KR100351620B1
公开(公告)日：2002-09-10
申请号：KR1019990027826
申请日：1999-07-09
Applicant: 한국생명공학연구원 , 주식회사대성미생물연구소
IPC: C12N9/14
CPC classification number: C12N9/16
Abstract: 본발명은 3차결정구조의내열성효소에관한것으로서, 더욱상세하게는, 4 내지 5개의안티-페러럴β-스트랜드(anti-parallel β-strand)로구성된블레이드 6개의내부에 3개의고친화칼슘부위(high-affinity calcium binding site)와 3개의저친화칼슘부위(low-affinity calcium binding site)를포함하는프로펠러형태의 3차결정구조를지닌내열성효소(thermo-stable enzyme)에관한것이다. 따라서, 상기의 Ca결합부위모티프를이용하면열 안정성이매우높은단백질합성에응용할수 있으며, 또한 3차구조로부터추정되는효소의활성부위또한최근에연구되는단백질공학기술로이러한효소활성부위를가진새로운효소를창출하는데 매우유용하게응용할수 있는효과가있다.
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公开(公告)号：KR1020010078497A
公开(公告)日：2001-08-21
申请号：KR1019990056274
申请日：1999-12-09
Applicant: 주식회사대성미생물연구소
IPC: A61K31/70
Abstract: PURPOSE: A complex antimicrobial composition for animals is provided, which has prevention and treatment effects on bacterial diseases and substitutes the expensive antimicrobial agents for animals. CONSTITUTION: The complex antimicrobial composition for animals comprises fluoroquinoline antimicrobial agents and chitosan oligosaccharides in a ratio of 1:10 to 10:1. Wherein, fluoroquinoline antimicrobial agents are enrofloxacin, ciprofloxacin and Norfloxacin; chitosan oligosaccharides have 2,000,000 to 150,000 molecular weight and 45% or more 1 to 16 saccharides; and the animals are selected from the group consisting of domestic animals comprising chickens, pigs, cows, horses, dogs, ducks and goats, fishes comprising trouts, carps, eels, flatfishes, sea bream and shrimps.
Abstract translation: 目的：提供用于动物的复合抗微生物组合物，其对细菌性疾病具有预防和治疗作用，并代替昂贵的抗微生物剂用于动物。 构成：用于动物的复合抗微生物组合物包含1:10至10:1比例的氟喹啉抗微生物剂和壳聚糖寡糖。 其中氟喹啉抗菌药是恩氟沙星，环丙沙星和诺氟沙星; 壳聚糖寡糖的分子量为2,000,000至150,000，糖分为45％或更多; 动物选自包括鸡，猪，牛，马，狗，鸭和山羊的家畜，包括鳟鱼，鲤鱼，鳗鱼，鲽鱼，鲷鱼和虾的鱼。
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公开(公告)号：KR1020010009461A
公开(公告)日：2001-02-05
申请号：KR1019990027826
申请日：1999-07-09
Applicant: 한국생명공학연구원 , 주식회사대성미생물연구소
IPC: C12N9/14
CPC classification number: C12N9/16
Abstract: PURPOSE: A thermo-stable enzyme having a three-dimensional crystal structure is provided which exhibits a high heat resistant property because calcium ion strongly links a number of peptide fragments together at a double-clasp and a central channel. Also the calcium ion plays a role in an increase of the enzyme activity by converting charge of an active site of the enzyme. CONSTITUTION: The propeller type of the thermo-stable enzyme comprises three high-affinity calcium binding sites and three low-affinity calcium binding sites inside six blades consisting of 4-5 of anti-parallel -strands. The thermo-stable enzyme is especially phytase derived from Bacillus amyloliquefaciens DS-II, and the six blades form a propeller type by being connected the 4th strand of each blade with upper region of the 1st strand of next blade. The extra -strand i. e., N-terminal segment is connected with the 5th blade.
Abstract translation: 目的：提供具有三维晶体结构的热稳定酶，其具有高耐热性，因为钙离子以双扣和中心通道将多个肽片段连接在一起。 此外，钙离子通过转化酶的活性位点的电荷而在酶活性的增加中起作用。 构成：热稳定酶的螺旋桨类型包括六个叶片内的三个高亲和力钙结合位点和三个低亲和力钙结合位点，其由4-5个反平行链组成。 热稳定酶特别是源自解淀粉芽孢杆菌DS-II的植酸酶，并且六叶片通过将每个叶片的第四股与下一个叶片的第一链的上部区域连接而形成螺旋桨型。 额外的品牌我 例如，N端子段与第五个叶片连接。
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公开(公告)号：KR1020000066478A
公开(公告)日：2000-11-15
申请号：KR1019990013597
申请日：1999-04-16
Applicant: 주식회사대성미생물연구소
IPC: A61K31/495
Abstract: PURPOSE: A complex antibiotic composition for animal is provided, which prevents and cures germ diseases of domestic animals and fishes, substitutes high cost of anti-biotic material, and minimizes remains of drug in an animals body. CONSTITUTION: The complex antibiotic composition for animal comprises of norfloxacin(NFLX) and tylosin(TYL) in the weight ratio of 1:4-4:1, desirably 1:2-2:1. The norfloxacin(NFLX) is a kind of third generation quinolone antibiotics, which inhibits DNA gyrases of a disease germ, so kills germs; which can be bound to DNA gyrases of a disease germ easily by carboxylic acid on No.3 position; and shows strong germicide effect by fluorine on No.6 position. The tylosin(TYL) inhibits translocation step of protein-synthesis by binding to a subunit of germ 50S ribosome, so obstructs protein-synthesis of a germ, and inhibits growth of a germ finally; which shows germicide action at high concentration.
Abstract translation: 目的：提供一种用于动物的复合抗生素组合物，可预防和治疗家畜和鱼类的细菌病，代替高成本的抗生物材料，并最大限度地减少动物体内的药物残留。 构成：动物复合抗生素组合物的重量比为1：4-4：1，理想的是1：2-2：1的诺氟沙星（NFLX）和泰乐菌素（TYL）。 诺氟沙星（NFLX）是一种第三代喹诺酮类抗生素，可以抑制疾病细菌的DNA旋转酶，从而杀死细菌; 其可以通过羧酸在3号位置容易地与疾病的DNA旋转酶结合; 氟在6号位置表现出强烈的杀菌作用。 泰乐菌素（TYL）通过与细菌50S核糖体的亚基结合来抑制蛋白质合成的易位步骤，从而阻碍细菌的蛋白质合成，最终抑制细菌的生长; 其显示高浓度的杀菌剂作用。
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55.发明授权
公开(公告)号：KR100206453B1
公开(公告)日：1999-07-01
申请号：KR1019970010948
申请日：1997-03-27
Applicant: 한국과학기술연구원 , 주식회사대성미생물연구소
CPC classification number: C12N9/16 , C12Y301/03008
Abstract: 본 발명은 신규한 플라즈미드를 이용하여 형질전환시킨 균주 대장균(E. coli) JM83/pKP2와 이로부터 생산된 피타제(Phytase)에 관한 것으로서, 더욱 상세하게는 단위 가축에 급여하는 곡류내 인(P)의 체내이용성을 높여주는 신규한 피타제의 대량생산을 위해 유전자서열을 규명하고, 이를 이용하여 유전자조작에 의해 신규한 재조합벡터 pKP1 또는 pKP2를 제조하며, 또한 이 신규한 재조합벡터 pKP1 또는 pKP2를 이용하여 대장균에 형질전환시킨 균주 E. coli JM83/pKP2에 관한 것이다.
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Patent Agency Ranking
公开(公告)号：KR100169913B1
公开(公告)日：1999-01-15
申请号：KR1019960006817
申请日：1996-03-14
Applicant: 한국과학기술연구원 , 주식회사대성미생물연구소
IPC: C12N1/20
CPC classification number: C12Y301/03008 , A23K20/189 , C12N9/16 , C12R1/07 , C12Y301/03026
Abstract: 본 발명은 신균주 바실러스속(Bacillus sp.) DS11(KCTC 0231BP)과 이로부터 생산되는 신규 파이타아제(Phytase)에 관한 것으로서, 더욱 상세하게는 신균주 바실러스속(Bacillus sp.) DS11과 단위가축에 급여하는 곡류내 인(P)의 체내 이용성을 높여주는 신규한 파이타아제 효소에 관한 것이다.
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公开(公告)号：KR1019970021288A
公开(公告)日：1997-05-28
申请号：KR1019950036731
申请日：1995-10-24
Applicant: 한국과학기술연구원 , 주식회사대성미생물연구소
Abstract: 본 발명은 신균주 페니실리움속(Penicillum sp.) AM301 (KCTC 8688P) 및 이로부터 생산되는 신규 파이타아제(Phytase)에 관한 것으로서, 더욱 상세하게는 신균주 페니실리움속(Penicillum sp.) AM301 (KCTC 8688P)과 단위가축에 급여하는 곡류내 인(P)의 체내이용성을 높여주는 신규한 파이타아제 효소에 관한 것이다.
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公开(公告)号：KR100092586B1
公开(公告)日：1995-12-06
申请号：KR1019910022989
申请日：1991-12-14
Applicant: 주식회사대성미생물연구소
IPC: C12P1/00
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公开(公告)号：KR100056599B1
公开(公告)日：1992-11-20
申请号：KR1019900011743
申请日：1990-07-31
Applicant: 주식회사대성미생물연구소
Inventor： 이동규
IPC: C12N7/04
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公开(公告)号：KR1019920009497B1
公开(公告)日：1992-10-17
申请号：KR1019910001232
申请日：1991-01-25
Applicant: 한국과학기술연구원 , 주식회사대성미생물연구소
IPC: C12N7/04
Abstract: A method for isolating Aujeszky's virus antigen protein comprises (a) adding phosphate buffer and sodium dodecyl sulfate into the culture soln. which obtd. by inoculating Aujeszky's virus AJHK 3 (KCTC VR 15001) into pig kidney cell, and (b) passing the soln. through cellulose sulfate gel to obtain antigen protein. Also claimed is a diagnostic kit for Aujeszky's disease which comprises polyethylene glycol, casein and Aujeszky's virus AJHK 3 (KCTC VR 15001) antigen protein.
Abstract translation: 用于分离Aujeszky病毒抗原蛋白的方法包括（a）向培养溶液中加入磷酸盐缓冲液和十二烷基硫酸钠。 哪个可以 通过将Aujeszky病毒AJHK 3（KCTC VR 15001）接种到猪肾细胞中，和（b）通过溶胶。 通过硫酸纤维素凝胶得到抗原蛋白。 还声称是Aujeszky病的诊断试剂盒，其包括聚乙二醇，酪蛋白和Aujeszky病毒AJHK 3（KCTC VR 15001）抗原蛋白。
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