公开(公告)号：EP1163497B1

公开(公告)日：2004-05-12

申请号：EP00915795.9

申请日：2000-02-18

Applicant: Packard Instrument Company, Inc.

Inventor： DUEBENDORFER, Juerg ,  JONES, Donald ,  NEUMANN, Kenneth ,  WANG, Chang

IPC: G01J3/30 ,  G01J1/58 ,  G01N21/25 ,  G01N21/64 ,  G01N35/02

CPC classification number: G01N21/6452 ,  G01N2201/0648

Abstract: An apparatus for measuring light in samples using a high intensity light source (12), is presented. The system utilizes bifurcated fiber bundle (26) to transmit light at the excitation and emission wavelength bands. It also uses a band-pass filter (36) for eliminating extraneous light, including that which corresponds to the excitation wavelength range, while permitting the emitted light to pass to a detector (41) for quantitation. The system employs a shutter (39) to shield the detector (41) while the laser light source is activated, and a controller to intermittently activate the laser light and close the shutter. The apparatus preferably includes lenses (16, 17, 37) for better illumination and read out conditions. The apparatus is employed in Luminescence Oxygen Channeling Immunoassays. The method has high sensitivity, accuracy and precision, and the apparatus is highly compact. Accordingly, the analyzer can perform assays in nanoliter to microliter sample volumes in standard microplates having at least 96, 384 or 1536 wells.

公开(公告)号：EP1099107A1

公开(公告)日：2001-05-16

申请号：EP99934902.0

申请日：1999-07-20

Applicant: PACKARD INSTRUMENT COMPANY

Inventor： RUSHBROOKE, John Gordon ,  HOOPER, Claire, Elizabeth

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/76 ,  G01N2021/6421 ,  G01N2021/6484 ,  G01N2201/0826 ,  G01N2201/0833 ,  G01N2201/0866

Abstract: Apparatus for detecting light emitted by assay samples is provided, in which light emitted by the sample is collected for transmission to a charge coupled device camera (74) by an optical fibre bundle. The cross-sectional area of the optical fibre bundle corresponds to the area of the sample, the end of which is located close to the sample for detecting any light emitted therefrom, and selected fibres (30) of those making up the bundle are separated from the remainder and extend to a source of excitation radiation (76) and serve to convey excitation radiation (if required) directly to a corresponding plurality of points distributed over the area of the end face of the bundle and therefore over the area of the sample. The remaining fibres (32, 38) of the bundle serve to collect emitted light (whether generated by fluorescence caused by excitation or otherwise) and provide a light path to the charge coupled device camera, wherein the ends of the excitation fibres and the ends of the emitted light collecting fibres are distributed uniformly over the area of the fibre bundle presented to the reaction site.

公开(公告)号：EP1007973A2

公开(公告)日：2000-06-14

申请号：EP98915459.6

申请日：1998-04-08

Applicant: Packard Instrument Company, Inc.

Inventor： PELC, Richard, E. ,  CHIBUCOS, Nicholas, S. ,  PAPEN, Roeland, F. ,  MAYER, Wilhelm

IPC: G01N35/10

CPC classification number: G01N35/1016 ,  B01J2219/00378 ,  C40B60/14 ,  G01F1/48 ,  G01F3/00 ,  G01N2035/00237 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1039 ,  G01N2035/1041

Abstract: A microvolume liquid handling system includes a microdispenser (16) employing a piezoelectric transducer (60) attached to a glass capillary (62), a positive displacement pump (12) for priming and aspirating transfer liquid into the microdispenser (16), controlling the pressure of the liquid system, and washing the microdispenser (16) between liquid transfers, and a pressure sensor (14) to measure the liquid system pressure and produce a corresponding electrical signal. The pressure signal is used to verify and quantify the microvolume of transfer liquid dispensed and is used to perform automated calibration and diagnostics on the microdispenser (16).

Abstract translation: 微量液体处理系统包括采用连接到玻璃毛细管（62）的压电换能器（60）的微分配器（16），用于将转移液体灌注并吸入微量分配器（16）的容积式泵（12），控制压力 并在液体传输之间清洗微分配器（16），以及压力传感器（14），以测量液体系统压力并产生相应的电信号。 压力信号用于验证和量化分配的转移液体的微量，并用于对微分配器（16）执行自动校准和诊断。

公开(公告)号：EP0610937B1

公开(公告)日：2000-04-26

申请号：EP94102080.2

申请日：1994-02-10

Applicant: Packard Instrument Company, Inc.

Inventor： Scheirer, Winfried

IPC: C12Q1/66

CPC classification number: C12Q1/66 ,  Y10S435/968 ,  Y10S530/802

公开(公告)号：EP0810438A2

公开(公告)日：1997-12-03

申请号：EP97108726.7

申请日：1997-05-30

Applicant: Packard Instrument Company, Inc.

Inventor： Pelc, Richard E. ,  Chibucos, Nicholas S. ,  Papen, Roeland F. ,  Meyer, Wilhelm J.

IPC: G01N35/10 ,  B01L3/02

CPC classification number: G01N35/1065 ,  B01J2219/00378 ,  B01L3/0268 ,  C40B60/14 ,  G01F1/48 ,  G01F3/00 ,  G01N2035/00237 ,  G01N2035/1018 ,  G01N2035/1039 ,  G01N2035/1041

Abstract: A low volume liquid handling system is described which includes a microdispenser employing a piezoelectric transducer attached to a glass capillary, a positive displacement pump for priming and aspirating transfer liquid into the microdispenser, controlling the pressure of the liquid system, and washing the microdispenser between liquid transfers, and a pressure sensor to measure the liquid system pressure and produce a corresponding electrical signal. The pressure signal is used to verify and quantify the microvolume of transfer liquid dispensed and is used to perform automated calibration and diagnostics on the microdispenser. In another embodiment of the low volume liquid handling system, a system reservoir is connected with tubing to a pressure control system for controlling the liquid system pressure in the system reservoir. The system reservoir is coupled to one or more microdispensers through a distribution tube having a branched section for each microdispenser. In this embodiment, each microdispenser is coupled to its own flow sensor and microvalve to enable a system controller to respectively measure and control the flow of liquid in the each microdispenser.

公开(公告)号：EP0660876A1

公开(公告)日：1995-07-05

申请号：EP93921526.0

申请日：1993-09-10

Applicant: Packard Instrument Company, Inc.

Inventor： ROELANT, Chris

IPC: C12Q1 ,  G01N33

CPC classification number: G01N33/5014 ,  C12Q1/06 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/904 ,  Y10S436/905

Abstract: The present invention provides a process of quantifying the number of viable cells in an aqueous suspension of cells using an energy-emitting non-hazardous probe and a probe-trigger. The process provides quantification data in short periods of time without the use of hazardous materials. A process of the present invention can also be used to quantify negatively charged particle number, assay for cytotoxicity, assay for cell proliferation and assay for cell differentiation. Still further, the present invention provides an assay kit for quantification of cells or negatively charged particles.

公开(公告)号：EP0610937A1

公开(公告)日：1994-08-17

申请号：EP94102080.2

申请日：1994-02-10

Applicant: Packard Instrument Company, Inc.

Inventor： Scheirer, Winfried

IPC: C12Q1/66

CPC classification number: C12Q1/66 ,  Y10S435/968 ,  Y10S530/802

Abstract: The present invention provides a method of increasing the duration of detectable photon emission of a luciferase-luciferin reaction. The method provides a luciferase-luciferin reaction in which photon emission can be detected for up to and including eight hours. A method of the present invention can also be used to detect the presence of luciferase in biological samples. The present invention also provides a composition used in detecting the presence of luciferase in biological samples.

Abstract translation: 本发明提供了增加荧光素酶 - 荧光素反应的可检测光子发射持续时间的方法。 该方法提供荧光素酶 - 荧光素反应，其中可以检测多达8个小时的光子发射。 本发明的方法也可用于检测生物样品中萤光素酶的存在。 本发明还提供了用于检测生物样品中荧光素酶的存在的组合物。

公开(公告)号：EP0421156A3

公开(公告)日：1991-07-31

申请号：EP90117473.0

申请日：1990-09-11

Applicant: Packard Instrument Company, Inc.

Inventor： Osten, Donald E.

IPC: G01N21/64

CPC classification number: G01N21/645 ,  G01N2021/516 ,  G01N2021/6469

Abstract: An improved method and apparatus is provided for performing high sensitivity fluorescence measurements. A liquid sample (12) contained within a cuvette (13) is exposed to exciting radiation in such a way that the radiation is introduced directly to the sample (12) and is restricted from reaching the container walls. The sample container (33) is in the form of a cylindrical cuvette provided with reflector means on the bottom so that any unabsorbed exciting radiation reaching the cuvette bottom is reflected back through the sample solution. Fluorescent radiation emitted by the sample (12) is collected by an ellipsoidal reflector (64) which substantially surrounds the sample container (33) and focuses radiation incident thereupon onto a small area from which it is efficiently sensed by a photodetector (66). The optical arrangement realizes significantly improved signal-to-noise and signal-to-background ratios.

公开(公告)号：EP0221626B1

公开(公告)日：1990-11-28

申请号：EP86303855.0

申请日：1986-05-21

Applicant: Packard Instrument Company, Inc.

Inventor： De Filippis, John Stanley

IPC: G01T1/204

CPC classification number: G01T1/2045 ,  G01T1/204

公开(公告)号：EP0334528A2

公开(公告)日：1989-09-27

申请号：EP89302483.6

申请日：1989-03-14

Applicant: Packard Instrument Company, Inc.

Inventor： Valenta, Robert J. ,  Noakes, John E.

IPC: G01T1/204

CPC classification number: G01T1/172 ,  G01T1/202 ,  G01T1/204

Abstract: A low-level liquid scintillation measurement system for counting sample optical events resulting from the radioactive decay of a constituent of a sample to be measured while reducing the counting of background optical events which are optical events produced by background radiation and which create electrical pulses other than pulses representing a sample optical event. This system includes detection means (12,14,16,18) located adjacent the sample for detecting optical events and for converting optical events into electrical pulses, coincidence sensing means (24) for receiving the electrical pulses from the detection means (12,14,16,18) and producing a trigger pulse when the electrical pulses from the detection means (12,14,16,18) coincide with each other, burst detection means (30) for receiving the trigger pulses from the coincidence sensing means (20) and the electrical pulses from the detection means (12,14,16,18) for determining the number of electrical pulses present in a selected interval following each of the trigger pulses, evaluation means (32) connected to the burst detection means (30) for determining, in response to the number of pulses detected in the selected interval, the extent to which the optical event represented by the corresponding trigger pulse should be treated as a sample optical event or a background event, and an active guard shield arrangement (100) comprising an auxiliary scintillator (104) optically coupled to the detection means (12,14,16,18). The shield (100) is adapted to be excited by background radiation and to effectively increase the number of electrical pulses present in the selected interval following a trigger pulse generated as a result of the coincident pulses produced due to the background radiation. This allows the measurement system to accurately evaluate the number of pulses detected in the selected interval and determine the extent to which a trigger pulse is to be treated as a sample optical event.

Abstract translation: 一种低级液体闪烁测量系统，用于计数由待测量的样品的成分的放射性衰变引起的样品光学事件，同时减少背景光学事件的计数，该背景光学事件是由背景辐射产生的光学事件，并产生除了 代表样品光学事件的脉冲。 该系统包括位于样本附近的用于检测光学事件并将光学事件转换成电脉冲的检测装置（12,14,16,18），符合检测装置（24），用于从检测装置（12,14）接收电脉冲 ，16,18），并且当来自检测装置（12,14,16,18）的电脉冲彼此一致时产生触发脉冲，用于从重合感测装置（20）接收触发脉冲的脉冲串检测装置（30） ）和来自检测装置（12,14,16,18）的电脉冲，用于确定在每个触发脉冲之后的选定间隔中存在的电脉冲数，连接到脉冲串检测装置（30）的评估装置（32） ），用于响应于在所选择的间隔中检测到的脉冲数来确定由对应的触发脉冲表示的光学事件应被视为样本光学事件或背景事件的程度，以及活动的海豹 d屏蔽装置（100），包括光学耦合到检测装置（12,14,16,18）的辅助闪烁体（104）。 屏蔽（100）适于被背景辐射激发，并且在作为由于背景辐射产生的重合脉冲的结果产生的触发脉冲之后有效地增加存在于所选间隔中的电脉冲的数量。 这允许测量系统准确地评估在所选间隔中检测的脉冲数，并确定将触发脉冲作为样本光学事件处理的程度。