公开(公告)号：KR101938711B1

公开(公告)日：2019-01-17

申请号：KR1020160124978

申请日：2016-09-28

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 김영옥 ,  남보혜 ,  김동균 ,  안철민 ,  공희정 ,  이종석 ,  김우중 ,  박해철 ,  김진희

IPC: A61K8/49 ,  A61K31/47 ,  A61K8/02 ,  A61Q19/02

公开(公告)号：KR1020160126204A

公开(公告)日：2016-11-02

申请号：KR1020150056985

申请日：2015-04-23

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 김영옥 ,  남보혜 ,  김동균 ,  안철민 ,  이종석 ,  김우중

IPC: C12N1/20 ,  C12P17/12 ,  B01D15/08 ,  C07D215/233

CPC classification number: B01D15/08 ,  C07D215/233 ,  C12N1/20 ,  C12P17/12

Abstract: 본발명은붉은멍게내장시료로부터분리된항균활성물질생산능이우수한미생물인신규슈도알테르모나스속 M2 균주, 상기균주로부터생산되는항균활성물질을제공한다.

Abstract translation: 本发明提供：一种新颖的拟前共生单胞菌 M2菌株，其是具有优异的抗菌材料生产能力并从卤虫的肠样品中分离的微生物; 由该菌株产生的基于假基的化合物; 和含有假戊基化合物作为活性成分的皮肤增白剂。

公开(公告)号：KR101618220B1

公开(公告)日：2016-05-18

申请号：KR1020140020365

申请日：2014-02-21

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 김영옥 ,  남보혜 ,  안철민 ,  이상준 ,  김동균 ,  지영주

IPC: C12N1/20 ,  C12R1/07

Abstract: 항균활성및 프로바이오틱스활성을갖는신규바실러스속균주가제공된다. 본발명의균주는다양한어류병원성세균뿐만아니라, 화장품표준균주및 치아우식균에대한광범위한항균활성을가지고있을뿐만아니라, 프로테아제, 아밀라제, 셀룰라제, 자일라제등 사료의소화흡수에도움을주는유용한효소를분비하는등 프로바이오틱스로서기능을하기때문에, 어류및 갑각류의사료첨가제는물론, 항균제와식품및 화장품분야의보존용첨가제로유용하게사용될수 있다.

公开(公告)号：KR101395942B1

公开(公告)日：2014-05-15

申请号：KR1020130030496

申请日：2013-03-21

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 남보혜 ,  김영옥 ,  김동균 ,  지영주 ,  이상준 ,  안철민

IPC: C07K14/46 ,  C12N15/12 ,  C12N15/63 ,  A61K38/17

CPC classification number: Y02A50/473

Abstract: The present invention relates to antimicrobial peptides, and provides an antibiotic peptide having a length of more than 12 amino acids selected from the amino acid sequence of SEQ ID: 3 and comprising the amino acid sequences of SEQ ID: 21 and uses thereof. According to an aspect of the present invention, a polynucleotide encoding an antimicrobial peptide according to an embodiment of the present invention is provided. In addition, a vector comprising a polynucleotide according to an embodiment of the present invention is provided.

Abstract translation: 本发明涉及抗微生物肽，并提供长度多于12个氨基酸的抗生素肽，其选自SEQ ID：3的氨基酸序列并且包含SEQ ID：21的氨基酸序列及其用途。 根据本发明的一个方面，提供了编码本发明实施方案的抗微生物肽的多核苷酸。 此外，提供了包含根据本发明实施方案的多核苷酸的载体。

公开(公告)号：KR1020090055457A

公开(公告)日：2009-06-02

申请号：KR1020080035162

申请日：2008-04-16

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 이상준 ,  김영옥 ,  남보혜

IPC: C12N15/64 ,  C12N15/09 ,  C12N15/10

CPC classification number: C12N15/62 ,  C12N15/625

Abstract: A method for producing a water soluble recombinant fusion protein from an exogenous gene and collecting a protein having an original amino terminal is provided to prevent insoluble precipitation of the recombinant protein and improve the secretion efficiency of the recombinant protein to cytoplasm outside or periplasm. A method for improving the secretion efficiency of a foreign protein comprises: a step of constructing a leader sequence containing N-region of the signal sequence and/or leader sequence of foreign protein, of which pI value is 9.90-11.28; a step of adjusting the distance of amino acid which makes an impact on pI value in the leader sequence; a step of constructing the leaser sequence, distance adjusting site, restriction enzyme recognition site and a polynucleotide coding fusion protein containing foreign protein; a step of inserting the constructed gene into an expression vector to produce a recombinant expression vector; a step of transforming a host cell with the recombinant expression vector; and a step of screening a transformant having high water-soluble expression.

Abstract translation: 提供从外源基因产生水溶性重组融合蛋白并收集具有原始氨基末端的蛋白质的方法，以防止重组蛋白的不溶性沉淀，并提高重组蛋白在细胞质外周或周质中的分泌效率。 提高外源蛋白质分泌效率的方法包括：构建含有pI值为9.90-11.28的信号序列的N-区和/或外来蛋白的前导序列的前导序列的步骤; 调整导致序列中对pI值产生影响的氨基酸的距离的步骤; 构建leaser序列，距离调整位点，限制酶识别位点和含有外源蛋白的多核苷酸编码融合蛋白的步骤; 将构建的基因插入表达载体以产生重组表达载体的步骤; 用重组表达载体转化宿主细胞的步骤; 以及筛选具有高水溶性表达的转化体的步骤。

公开(公告)号：KR1020060111144A

公开(公告)日：2006-10-26

申请号：KR1020050033562

申请日：2005-04-22

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 강정하 ,  남보혜 ,  이상준 ,  김봉석 ,  김종현 ,  김경길

IPC: C12Q1/68 ,  C12N15/12

CPC classification number: C12Q1/6844 ,  C12N15/11 ,  C12Q1/70 ,  C12Q2521/101 ,  C12Q2600/158

Abstract: A method for discrimination of individuals infected with lymphocystis disease virus(LCDV) or Paralichthys olivaceus individuals having resistance against lymphocystis disease virus is provided to produce of healthy individuals without virus infection, reduce lymphocystis disease occurrence rate by treating lymphocystis disease at an early stage, and selectively produce the excellent Paralichthys olivaceus individuals. A genetic marker for discrimination of fish individuals infected with lymphocystis disease virus has the nucleotide sequence of SEQ ID NO:10, wherein the genetic marker is amplified by a pair of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, specific to NIF/NLI interacting factor gene; and the fish is Paralichthys olivaceus. The method for discrimination of fish individuals infected with lymphocystis disease virus comprises the steps of: (i) extracting the DNA sample of fish to be discriminated; (ii) amplifying the DNA sample with the primer pair of SEQ ID NO:1 and SEQ ID NO:2; and (iii) analyzing the amplification of the NIF/NLI interacting factor gene that is specific to the infection of lymphocystis disease virus.

Abstract translation: 提供一种鉴别感染淋巴囊病病毒（LCDV）或具有抗淋巴囊病病毒的Paralichthys olivaceus个体的个体的方法，以产生没有病毒感染的健康个体，通过早期治疗淋巴囊肿病减少淋巴囊肿病发生率，以及 选择性地产生优良的Paralichthys olivaceus个体。 用于鉴定感染淋巴囊病病毒的鱼类个体的遗传标记具有SEQ ID NO：10的核苷酸序列，其中遗传标记通过具有SEQ ID NO：1和SEQ ID NO：1的核苷酸序列的一对引物扩增。 2，特异性NIF / NLI相互作用因子基因; 鱼是Paralichthys olivaceus。 用于鉴别感染淋巴囊病病毒的鱼类个体的方法包括以下步骤：（i）提取待鉴别的鱼的DNA样品; （ii）用SEQ ID NO：1和SEQ ID NO：2的引物对扩增DNA样品; 和（iii）分析淋巴囊性疾病病毒感染特异的NIF / NLI相互作用因子基因的扩增。