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    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질
    51.
    发明授权
    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질 有权
    使用丝朊蛋白和纯化蛋白质的蛋白质的质量纯化方法

    公开(公告)号：KR101346057B1

    公开(公告)日：2013-12-31

    申请号：KR1020120068585

    申请日：2012-06-26

    Applicant: 대한민국(농촌진흥청장) ,  차의과학대학교 산학협력단

    Inventor： 이희삼 ,  조유영 ,  권해용 ,  남성희 ,  류강선 ,  강필돈 ,  박광영 ,  김미자 ,  최용수 ,  김호진 ,  황정욱

    IPC: C07K1/14 ,  C07K14/435 ,  C12N1/04

    Abstract: The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount

    Abstract translation: 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括：冷冻干燥蚕的第一步骤，制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步，使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明，蚕血淋巴可应用于细胞冷冻保存剂，细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的，因此本发明有助于增加蚕食农民的收入。 （参考号）（AA）丝腺去除;（BB）成熟蚕;（CC）活体重3.13g;（DD）离心;（EE）快速冷冻;（FF）成熟蚕冷冻590 （GG）粉碎/选择;（HH）血淋巴380mg;（II）溶解（蒸馏水）;（JJ）热处理后63mg;（KK）30k 70k分离;（LL）最终检索量

    귀뚜라미 추출물을 함유하는 간보호 효과, 강장효과,알코올해독, 과산화지질 생성 억제용 약학적 조성물
    55.
    发明授权
    귀뚜라미 추출물을 함유하는 간보호 효과, 강장효과,알코올해독, 과산화지질 생성 억제용 약학적 조성물 有权
    衍生自蟋蟀的药物组合物，发挥肝保护，抗疲劳，增​​强乙醇降解的活性，抑制脂质过氧化的形成

    公开(公告)号：KR100498809B1

    公开(公告)日：2005-07-01

    申请号：KR1020030094612

    申请日：2003-12-22

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 안미영 ,  류강선 ,  이용우 ,  김영식 ,  이용기 ,  이희삼 ,  김은선 ,  김익수

    IPC: A61K35/64

    Abstract: 본 발명은 귀뚜라미 추출물 함유 간보호 효과, 강장피로회복, 알콜해독, 과산화지질 생성억제 용도 의약품 조성물에 관한 것으로서,
    본발명에 따른 귀뚜라미 추출물은 급성간염 유발시 GOT, GPT, LDH 등 간 혈청 효소를 기존의 실리마린 대비 116%이상 회복하고, 과산화지질의 생성를 정상군 대비 140% 억제 효과를 포함한다. 또한 알콜 섭취 시 귀뚜라미 엑기스를 1시간 전에 복용하였을 때 혈청 중의 에탄올 함량이 114% 이하 감소 및 귀뚜라미 수용성 추출 분획에서는 혈청 내 알콜탈수소효소의 높은 증가(216% 이상)와 알코올 추출 분획에 있어서 미토콘드리아 내 아세트 알데하이드 탈수소효소의 높은 증가(491%)을 특징으로 한다.

    흰점박이꽃무지 추출물 및 이의 용도
    56.
    发明公开
    흰점박이꽃무지 추출물 및 이의 용도 有权
    PROTAETIA BREVITARSIS提取及其使用

    公开(公告)号：KR1020030001659A

    公开(公告)日：2003-01-08

    申请号：KR1020010036463

    申请日：2001-06-26

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이희삼 ,  김선여 ,  류강선 ,  김진원 ,  오영륜 ,  여주홍

    IPC: A61K35/64 ,  A23L1/30

    CPC classification number: A23L33/10 ,  A61K35/63

    Abstract: PURPOSE: An extract of Protaetia brevitarsis which has almost the same liver protecting effect as silymarin as a conventional liver disease treating agent is provided which can be effectively used as an effective ingredient for a liver disease treating agent or health supplementary agent. CONSTITUTION: Distilled water is added to larvae powder of Protaetia brevitarsis and extracted and the extract is passed through a dialysis membrane to produce a Protaetia brevitarsis extract having a molecular weight of 1,000 or less. The extract is formulated to be administered in a dose of 1 to 2,500mg/kg/day, preferably 10 to 500mg/kg/day and can recover the liver effectively when hepatotoxicity is induced by drugs such as carbon dioxide.

    Abstract translation: 目的：提供与常规肝脏疾病治疗剂几乎与水飞蓟素相同的肝保护作用的红曲霉提取物，其可以有效地用作肝脏疾病治疗剂或健康补充剂的有效成分。 构成：将蒸馏水加入到短白茅的幼虫粉末中，萃取，将提取物通过透析膜，制成分子量为1000以下的短叶提取物。 将提取物配制成以1-2500mg / kg /天，优选10-500mg / kg /天的剂量施用，并且当药物如二氧化碳诱导肝毒性时，可以有效地回收肝脏。

    기능성 항당뇨음료
    57.
    发明授权
    기능성 항당뇨음료 有权
    功能性抗菌饮料

    公开(公告)号：KR100354151B1

    公开(公告)日：2002-09-28

    申请号：KR1019990061216

    申请日：1999-12-23

    Applicant: 최진호 ,  대한민국(농촌진흥청장)

    Inventor： 최진호 ,  류강선 ,  이회삼 ,  김대익 ,  박수현 ,  김동우

    IPC: A23L2/38

    CPC classification number: A61K36/9068 ,  A61K35/63 ,  A61K36/54 ,  A61K2300/00

    Abstract: 본발명은기능성항당뇨음료에관한것으로당뇨병발병을예방하고합병증을치료할수 있는누에가루를메탄올로추출한누에주출물(MLE)을사용한것이다. 본발명은 5령 3일의누에를동결건조하고, 이것을다시메탄올로추출하여감압농축한다음, 다시동결건조한생리활성성분이완전히보존된누에추출물 (SWE) 0.01∼15.0중량부에이취제거제로서생강추출물 0.001∼1.0중량부및 계피추출물 0.001∼2.50중량부를첨가하여이취를순화하고, 식미및 기호강화제로서구연산 0.001∼1.0중량부, 비타민 C 0.001∼1.0중량부를첨가하고, 여기에항피로제로서타우린 0.01∼10.0중량부를첨가ㆍ혼합한다음, 1,000×g에서 10분간원심분리하여상층액을사용하여전체 100ml되도록한 기능성항당뇨음료이다.

    뽕잎을 주재로 한 아이스크림류의 제조방법
    58.
    发明公开
    뽕잎을 주재로 한 아이스크림류의 제조방법 有权
    使用大豆叶的冰淇淋的制备方法

    公开(公告)号：KR1020010016895A

    公开(公告)日：2001-03-05

    申请号：KR1019990032108

    申请日：1999-08-05

    Applicant: 대한민국(농촌진흥청장) ,  홍영식품 주식회사

    Inventor： 류강선 ,  김현복 ,  김선여 ,  이희삼 ,  이용기 ,  이용우 ,  임수호 ,  정운영

    IPC: A23G9/04

    CPC classification number: A23G9/42

    Abstract: PURPOSE: A method for preparing a kind of ice cream containing powdered mulberry leaves and optionally milk fat is provided to serve low-caloric ice cream products having unique flavor, color and texture and preventing geriatric diseases. CONSTITUTION: The ice cream product containing milk fat is prepared by the steps of: (i) dipping mulberry leaves into an aqueous solution of sodium bicarbonate for 5sec and pulverizing the leaves to fine powder of 100-300mesh; (ii) leaving mulberry leaves in an airtight space filled with nitrogen gas, drying and washing the leaves before grinding the processed leaves to fine powder and adding to the mulberry leaf powder above in a ratio of 1:1; (iii) mixing 18-35wt% of raw cream, 7-11wt% of powdered nonfat milk, 4-7wt% of ice sugar, 0-3wt% of hydrous crystalline dextrose, 0.2-0.6wt% of emulsion stabilizer, 0-0.2wt% of vanilla, 0-0.05wt% of refined salt, 9-13wt% of refined sugar and refined water at 40-90deg.C for 10-60min; (iv) homogenizing and sterilizing the mixture at 70-100deg.C for 10-60sec before cooling and adding to 0.1-5wt% of the powdered mulberry leaves; and then (v) aging the mixed mulberry leaf powder at 1-10deg.C for 1-20hr and increasing the quantity of the aged to 50-150 times and frozen mixture before packing the resulting product. The ice cream not containing milk fat comprises 0.1-5wt% of the powdered mulberry leaves, 9-12wt% of powdered nonfat milk, 9.5-12% of coconut hardened oil, 4-8wt% of ice sugar, 0-4wt% of hydrous crystalline dextrose, 0.2-0.6wt% of emulsion stabilizer, 0-0.2wt% of vanilla, 0-0.05wt% of refined salt, and refined water. The emulsion stabilizer includes 22.5% of guar gum, 19.5% of locust bean gum, 4.5% of carrageenan, 3.5% of Tamarind gum and 50% of Glycerin fatty acid ester.

    Abstract translation: 目的：提供一种制备含有桑叶和任选的乳脂的冰淇淋的方法，用于提供具有独特风味，色泽和质感的低热量冰淇淋产品，并防止老年病。 构成：含有乳脂的冰淇淋产品是通过以下步骤制备的：（i）将桑叶浸入碳酸氢钠水溶液中5秒钟，粉碎至100-300目的细粉; （ii）将桑叶留在装有氮气的气密空间中，干燥和洗涤叶子，然后将加工的叶子研磨成细粉末，并以1:1的比例加入到上述桑叶粉末中; （iii）混合18-35重量％的生奶油，7-11重量％的粉状脱脂牛奶，4-7重量％的冰糖，0-3重量％的含水结晶葡萄糖，0.2-0.6重量％的乳化稳定剂，0-0.2 重量百分比的香草，0-0.05重量％的精制盐，9-13重量％的精制糖和精制水在40-90℃下进行10-60分钟; （iv）将混合物在70-100℃均化灭菌10-60秒，然后冷却，加入0.1-5重量％的桑叶粉末; 然后（v）将混合的桑叶粉末在1-10℃下老化1-20小时，并将包装所得产物的老化量增加到50-150倍，冷冻混合物。 不含乳脂的冰淇淋含有0.1-5重量％的桑叶粉，9-12重量％粉末脱脂奶，9.5-12％椰子硬化油，4-8重量％冰糖，0-4重量％含水量 结晶葡萄糖，0.2-0.6重量％的乳化稳定剂，0-0.2重量％的香草，0-0.05重量％的精制盐和精制水。 乳化稳定剂包括22.5％的瓜尔胶，19.5％的刺槐豆胶，4.5％的角叉菜胶，3.5％的罗望子胶和50％的甘油脂肪酸酯。

    누에를 이용한 골다공증 예방 및 개선용 식품 조성물 및 이를 함유하는 건강보조식품
    59.
    发明公开
    누에를 이용한 골다공증 예방 및 개선용 식품 조성물 및 이를 함유하는 건강보조식품 有权
    食品组合物，用于预防和改善使用丝状蛋白的食管和包括其的食物

    公开(公告)号：KR1020130141964A

    公开(公告)日：2013-12-27

    申请号：KR1020120065132

    申请日：2012-06-18

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 류강선 ,  이희삼 ,  강필돈 ,  김기영 ,  김미자

    IPC: A23L33/00 ,  A23L3/40 ,  A23L2/38 ,  A23P1/02

    CPC classification number: A23L33/10 ,  A23L2/38 ,  A23L33/17 ,  A23P10/20 ,  A23P10/28 ,  A23V2002/00 ,  A23V2200/306

    Abstract: The present invention relates to a food composition for prevention and improvement of osteoporosis using a silkworm and a food containing the same. The food composition for prevention and improvement of osteoporosis includes power of female silkworms, and the species of the silkworm is a yellow-green silkworm. According to the present invention, the usability of a female silkworm is improved and a composition for prevention and improvement of osteoporosis using a silkworm which has a superior prevention and improvement effect on osteoporosis and a functional food containing the same are provided.

    Abstract translation: 本发明涉及使用蚕和含有蚕的食品预防和改善骨质疏松症的食品组合物。 用于预防和改善骨质疏松症的食物成分包括女性蚕的力量，蚕的种类是黄绿蚕。 根据本发明，提高了女性蚕的使用性，并且提供了使用对骨质疏松症具有优异的预防和改善作用的蚕的骨质疏松症的预防和改善的组合物以及含有该成分的功能性食品。

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