Abstract:
본 발명은 실크단백질을 이용한 치주 골조직유도 재생용 차폐막 및 그 제조방법에 관한 것이다. 본 발명의 치주 골조직유도 재생용 차폐막의 제조방법은 실크 단백질로부터 세리신을 제거하여 실크 피브로인을 얻는 단계; 상기 실크 피브로인을 염을 포함한 에탄올수용액으로 용해시켜 실크 피브로인 염 에탄올수용액을 제조하는 단계; 상기 실크 피브로인 염 에탄올수용액으로부터 염과 에탄올을 제거하여 실크 피브로인 수용액을 제조하는 단계;및 상기 실크 피브로인 수용액을 평평한 용기에 붓고 건조하는 단계;를 포함한다. 본 발명에 의해, 상처 부위의 밀착성이 우수할 뿐만 아니라 골조직 재생성도 우수하고 생체내 생분해성을 갖는 치주 골조직유도 재생용 차폐막 및 그 제조방법이 제공된다.
Abstract:
PURPOSE: An injection method of cordyceps spawns to silkworm pupae, and a cultivation method of cordyceps using the same are provided to improve the productivity of the cordyceps. CONSTITUTION: An injection method of cordyceps spawns to silkworm pupae comprises the following steps: storing cordyceps liquid spawns in a container; inserting silkworm pupae into the cordyceps liquid spawns, and soaking for 0.5-3 minutes; and sealing the container, and impregnating the cordyceps liquid spawns into the silkworm pupae with the pressure of 0.5 ~ 2kg per square centimeter.
Abstract translation:目的:采用冬虫夏草注射法产蚕蛹,并提供冬虫夏草的培养方法,以提高冬虫夏草的生产率。 构成:冬虫夏草诱导蚕蛹的注射方法包括以下步骤:将虫草液体产卵储存在容器中; 将蚕蛹插入冬虫夏草液中,浸泡0.5-3分钟; 并密封容器,并以0.5〜2kg / cm 2的压力将冬虫夏草液体产卵浸入蚕蛹中。
Abstract:
PURPOSE: A porous 3-dimensional scaffold is provided to improve the effect of tissue cells attached to the surface of the scaffold penetrating inside the scaffold, thereby improving recovery effectiveness. CONSTITUTION: A manufacturing method of a porous 3-dimensional scaffold comprises: a step of manufacturing agarose gel through hydrogelation of agarose to liquid state after preparing agarose which is pore introducing material; a step of press-molding by putting the agarose gel into a mold with base material of biomaterial forming scaffold; a step of quenching and freeze-drying the molded product , and fixing the product by ethanol; and a step of manufacturing a 3-deminsional scaffold with improved porosity and air-permeability by removing agarose by soaking the product to water with the temperature of 50 °C or more.
Abstract:
PURPOSE: A three dimensional scaffold using low molecular silk fibroin and agarose gel is provided to develop artificial bone or a material for developing tissue regeneration. CONSTITUTION: A three dimensional scaffold contains 0.01-0.05 wt% of low molecular silk fibroin and 1.0 wt% of agarose gel. A method for manufacturing the three dimensional scaffold comprises: a step of preparing agarose and gelling in a liquid state to prepare agarose gel; a step of adding low molecular silk fibroin powder and mixing; and a step of injecting the mixture into a frame and freeze-drying.