公开(公告)号：KR1020150087542A

公开(公告)日：2015-07-30

申请号：KR1020140007628

申请日：2014-01-22

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  정현숙 ,  이준호

IPC: C07K7/08 ,  A61K38/10 ,  A61P31/10

CPC classification number: Y02A50/473 ,  Y02A50/481

Abstract: 본발명은솔잎으로부터분리된신규한항생펩타이드 PN-5 및이의용도에관한것으로, 구체적으로솔잎에서분리한서열번호 1로기재되는아미노산서열을가지는신규한펩타이드가병원성균주및 식중독균주에대하여현저한항진균효과를나타낸고, 세포독성이없는것을확인함으로써, 상기솔잎으로부터분리된신규한항생펩타이드 PN-5는항생용약학적조성물, 화장료조성물, 농약, 건강식품, 방부용조성물및 의약의품으로유용하게사용할수 있다.

Abstract translation: 本发明涉及从松针分离的新型抗微生物剂PN-5肽及其用途。 更具体地说，本发明证实具有SEQ ID NO：1所示的氨基酸序列的新型肽显示对致病菌株和食物中毒致病菌株的显着的抗真菌作用，并且不具有细胞毒性。 因此，从松针分离的新型抗微生物肽可以用作抗微生物剂和药物组合物，化妆品组合物，农药，膳食补充剂，防腐剂组合物和准。

公开(公告)号：KR1020130100575A

公开(公告)日：2013-09-11

申请号：KR1020120021908

申请日：2012-03-02

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  박성철

IPC: A61K38/17 ,  C07K14/47 ,  A01N63/02 ,  A61P31/04

CPC classification number: Y02A50/473 ,  Y02A50/481 ,  A61K38/1709 ,  A01N63/02 ,  A23V2200/10 ,  A61K8/981 ,  A61K2800/524 ,  A61Q17/005 ,  C07K14/47

Abstract: PURPOSE: A protein with antibacterial activity isolated from human amniotic fluid is provided to show antibacterial activity against harmful bacteria and to be used as a cosmetic product and a pharmaceutical preservative. CONSTITUTION: An antibacterial and antiseptic composition contains a protein of sequence number 1 with antibacterial activity as an active ingredient. The protein is derived from human amniotic fluid. The protein has antibacterial activity against one selected among Staphylococcus aureus, Listeria monocytogenes, Staphylococcus epidermidis, Escherichia coli, Proteus vulgaris, and Salmonella typhimurium. An agrochemical formulation contains the proteins as an active ingredient. A cosmetic composition with antibacterial activity contains the proteins as an active ingredient.

Abstract translation: 目的：提供从人类羊水中分离的具有抗菌活性的蛋白质，以显示对有害细菌的抗菌活性，并用作化妆品和药物防腐剂。 构成：抗菌消毒组合物含有具有抗菌活性的活性成分的序列号1的蛋白质。 蛋白质来源于人类羊水。 该蛋白质对金黄色葡萄球菌，单核细胞增多性李斯特菌，表皮葡萄球菌，大肠杆菌，普通变形杆菌和鼠伤寒沙门氏菌中的一种具有抗菌活性。 农药制剂含有蛋白质作为活性成分。 具有抗菌活性的化妆品组合物含有蛋白质作为活性成分。

公开(公告)号：KR1020130080172A

公开(公告)日：2013-07-12

申请号：KR1020120000952

申请日：2012-01-04

Applicant: 조선대학교산학협력단

Inventor： 박윤경

IPC: C07K14/415 ,  C07K1/16 ,  A61K38/16 ,  A61P31/10

Abstract: PURPOSE: A novel antifungal peptide isolated from Solanum tuberosum L cv. Jopung is provided to have an antifungal activity against Candida albicans and to be used in pharmaceutical compositions, cosmetic compositions, agrochemicals, preservatives for foods, cosmetics, and pharmaceutical products. CONSTITUTION: An antifungal peptide has an amino acid sequence of sequence number 1 and is isolated from Solanum tuberosum L cv. Jopung. The molecular weight of the antifungal peptide is 1.0831 kDa. A method for preparing the peptide comprises the steps of: freeze-drying and pulverizing bulbs of Solanum tuberosum L cv. Jopung; extracting the pulverized bulbs and preparing an extract; centrifuging the extract and collecting a supernatant fraction; and isolating and purifying the supernatant fraction by chromatography. An antifungal pharmaceutical composition contains the peptide as an active ingredient.

Abstract translation: 目的：从Solanum tuberosum L cv。中分离出一种新颖的抗真菌肽。 提供Jopung具有抗白色念珠菌的抗真菌活性，并用于药物组合物，化妆品组合物，农业化学品，食品防腐剂，化妆品和药品中。 构成：抗真菌肽具有序列号1的氨基酸序列，并从马铃薯（Solanum tuberosum L cv。）中分离。 Jopung。 抗真菌肽的分子量为1.0831kDa。 制备肽的方法包括以下步骤：冷冻干燥和粉碎马铃薯薯片的鳞茎。 Jopung; 提取粉碎的球茎并制备提取物; 离心提取物并收集上清液部分; 并通过色谱分离和纯化上清液级分。 抗真菌药物组合物含有肽作为活性成分。

公开(公告)号：KR101256943B1

公开(公告)日：2013-04-26

申请号：KR1020100080012

申请日：2010-08-18

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  김시욱 ,  박성철

IPC: C07K7/08 ,  C07K14/435 ,  A01N63/02

Abstract: 본 발명은 적조 방제 활성을 갖는 항적조 펩타이드 P5에 관한 것으로서, 보다 구체적으로 본 발명에 의한 항적조 펩타이드는 적조 유발 조류에 대하여 탁월한 항적조 활성을 나타내고, 유익한 조류에는 독성이 없으며, 자연 분해가 가능하므로 해양 생태계에 안전하고 유용하게 사용될 수 있다.

公开(公告)号：KR1020120138432A

公开(公告)日：2012-12-26

申请号：KR1020110057907

申请日：2011-06-15

Applicant: 조선대학교산학협력단 ,  강원대학교산학협력단 ,  주식회사 바이오리더스 ,  국민대학교산학협력단

Inventor： 박윤경 ,  채병조 ,  홍승표 ,  성문희 ,  함경수

IPC: A23K20/195 ,  A23K1/18 ,  C07K19/00

CPC classification number: A23K20/147 ,  A23K50/60 ,  A23K50/75 ,  C07K19/00

Abstract: PURPOSE: A feed additive containing P5 peptide for replacing antibiotics, and a producing method thereof are provided to use the feed additive for weaned piglets or meat chicken. CONSTITUTION: A feed additive containing P5 peptide for replacing antibiotics contains 0.30-0.50 parts of antimicrobial P5 peptide by weight, 3,300-3,500 kcal/kg of energy, 21.0-23.0 parts of crude protein by weight, and 1.0-1.6 parts of lysine by weight. The feed additive is for feed of weaned piglets. 0.30-0.50 parts of feed additive by weight is mixed with the total amount of feed. [Reference numerals] (AA) Peptide; (BB) Amino acid sequence; (CC) Property; (DD) Parent peptide; (EE) CA-MA: C-terminal removal (HSAKKF); (FF) P1:N-terminal addition; (GG) P1:C-terminal addition; (HH) CA-MA: C-terminal removal and substitution; (II) CA-MA: substitution

Abstract translation: 目的：提供含有用于替代抗生素的P5肽的饲料添加剂及其制备方法，用于断奶仔猪或肉鸡的饲料添加剂。 构成：含有用于替代抗生素的P5肽的饲料添加剂含有重量为0.30-0.50份抗微生物P5肽，3,300-3,500千卡/ kg能量，粗蛋白质重量为21.0-23.0份，赖氨酸为1.0-1.6份 重量。 饲料添加剂用于饲喂断奶仔猪。 将0.30-0.50份饲料添加剂与饲料总量混合。 （AA）肽; （BB）氨基酸序列; （CC）财产; （DD）亲本肽; （EE）CA-MA：C-末端去除（HSAKKF）; （FF）P1：N-末端加成; （GG）P1：C-末端加成; （HH）CA-MA：C-末端去除和取代; （二）CA-MA：取代

公开(公告)号：KR1020100094155A

公开(公告)日：2010-08-26

申请号：KR1020090013448

申请日：2009-02-18

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  함경수 ,  김시욱 ,  박성철

IPC: A01N63/02 ,  A01P21/00

CPC classification number: A01N63/02 ,  C07K1/10 ,  C07K1/14 ,  C07K14/46

Abstract: PURPOSE: A peptide for preventing red tide is provided to ensure excellent anti-tidal activity without cytotoxicity and enable natural decomposition. CONSTITUTION: A peptide for preventing red tide has an amino acid which is substituted from positive charge to negative charge in a helix peptide with antibiotic activity. The amino acid with positive charge is a hydrophilic part. A peptide of helix structure with antibiotic activity is an amino acid of sequence number 1. A peptide for preventing red tide is an amino acid of sequence number 2. A composition for preventing red tide contains a peptide of sequence number 2 as an active ingredient. The composition has prevention activity to Alexandrium sp., Alexandrium catanella, Skeletonema costatum, or Prorocentrum minimum.

Abstract translation: 目的：提供一种防止红潮的肽，以确保优异的抗潮汐活性而无细胞毒性，并能自然分解。 构成：用于预防红潮的肽具有在具有抗生素活性的螺旋肽中具有由正电荷取代为负电荷的氨基酸。 具有正电荷的氨基酸是亲水部分。 具有抗生素活性的螺旋结构的肽是序列号1的氨基酸。用于预防红潮的肽是序列号2的氨基酸。用于预防红潮的组合物含有序列号2的肽作为活性成分。 该组合物具有对亚历山大藻，亚历山大藻，克氏骨髓或最小起搏的预防活性。

公开(公告)号：KR100935031B1

公开(公告)日：2010-01-06

申请号：KR1020070088127

申请日：2007-08-31

Applicant: 조선대학교산학협력단

Inventor： 함경수 ,  박윤경 ,  박성철 ,  김미현

IPC: C07K14/195 ,  C07K14/205

Abstract: 본 발명은 헬리코박터 파일로리균의 리보좀 단백질 L1 유래의 유사체 펩타이드로부터 풀림구조를 절단한 양이온 증가 항생 펩타이드 및 항균 및 항진균용 조성물에 관한 것으로, 서열번호 2로 기재되는 아미노산 서열을 가지는 HPA3의 N-말단의 1번부터 4번까지 풀림구조를 절단하고 9번째와 13번째 아미노산인 글루탐산과 세린을 리신으로 치환시킴으로써 풀림구조를 절단시켜 펩타이드의 나선구조만 남기고 양이온 성질을 증가시킴으로써 서열번호 3으로 기재된 항생 펩타이드 및 그를 포함하는 조성물에 관한 것이다. 구체적으로 본 발명의 항생 펩타이드는 헬리코박터 파일로리(
Helicobacter

pylori ) 균의 리보좀 단백질 L1(ribosomal protein L1, RPL1) 아미노말단 부위에서, 양친화성 (amphipathic)과 항생 활성을 갖는 HP(2-20) 펩타이드의 소수성 부위를 증가시킨 HPA3로부터, N-말단의 1번부터 4번까지 풀림구조를 절단하고 5번째와 9번째 아미노산인 글루탐산과 세린을 리신으로 치환시킴으로써 세포의 막에 잘 결합하도록 하는 구조를 갖게 됨으로 탁월한 항균 및 항진균 활성을 나타내고 세포 독성이 없으므로 인체에 안전한 항생제로 유용하게 사용될 수 있다.
헬리코박터 파일로리균, 리보좀 단백질 L1, 항생 펩타이드, 나선 구조, 양이 온

公开(公告)号：KR1020090022608A

公开(公告)日：2009-03-04

申请号：KR1020070088127

申请日：2007-08-31

Applicant: 조선대학교산학협력단

Inventor： 함경수 ,  박윤경 ,  박성철 ,  김미현

IPC: C07K14/195 ,  C07K14/205

Abstract: A cationic increment antibiotic peptide produced by cleaving a non-native structure of an analogs peptide derived from ribosomal protein l1 of helicobacter pylori is provided to improve cationic properties while leaving a helical structure of an analogs peptide derived from ribosomal protein l1 of helicobacter pylori. A method for producing a cationic increment antibiotic peptide comprises the following steps of: cleaving 1st~4th amino acids of HPA3(Helicobacter pylori Analogue 3) having an amino acid sequence indicated as sequence number 2; and substituting a positive charge amino acid for the 9th amino acid of glutamic acid and the 13th amino acid of serine. The positive charge amino acid is selected from the group consisting of lysine, arginine, and histidine.

Abstract translation: 提供通过切割衍生自幽门螺杆菌的核糖体蛋白质11的类似物肽的非天然结构产生的阳离子增量抗生素肽，以改善阳离子性质，同时留下衍生自幽门螺杆菌的核糖体蛋白质11的类似物肽的螺旋结构。 制备阳离子增量抗生素肽的方法包括以下步骤：切割具有序列号2表示的氨基酸序列的HPA3（幽门螺杆菌模拟物3）的第1-4位氨基酸; 用正电荷氨基酸取代谷氨酸的第9个氨基酸和丝氨酸的第13个氨基酸。 正电荷氨基酸选自赖氨酸，精氨酸和组氨酸。

公开(公告)号：KR1020080044689A

公开(公告)日：2008-05-21

申请号：KR1020060114038

申请日：2006-11-17

Applicant: 조선대학교산학협력단

Inventor： 함경수 ,  박윤경 ,  박성철

IPC: C12N1/20

Abstract: A novel peptide isolated from a novel Bacillus subtilis S1 is provided to show excellent antibiotic effect on harmful fungi and bacteria, thereby being usefully used as an antibacterial and anti-fungal pharmaceutical composition, a pesticide, or a preservative of food, cosmetics or medicines. A Bacillus subtilis S1 having the antibiotics activity is deposited as a deposition no. KCTC 10888BP. A method for purifying a peptide from the Bacillus subtilis comprises the steps of: (a) culturing the Bacillus subtilis S1; (b) after filtering a culture solution obtained from the step(a), loading the filtrate into RP-HPLC(reverse phase-high performance liquid chromatography); (c) eluting proteins to a column of the step(b) using an eluent with gradually increased acetonitrile concentration; and (d) recovering a peak fraction from the eluent and investigating the antibacterial activity thereof. A peptide purified by the method and showing anti-bacterial and anti-fungal activities is characterized in that it is secreted from the Bacillus subtilis S1, has a molecular weight of 1.4-1.6 KDa, and shows antibacterial activity on Rhizoctonia solani and Colletotrichum coccodes. An anti-bacterial and anti-fungal pharmaceutical composition, a biological pesticide or a preservative of foods, cosmetics, or medicines comprises the peptide as an effective ingredient. Further, the concentration of the acetonitrile increases from 5% to 65%.

Abstract translation: 提供从新型枯草芽孢杆菌S1分离的新肽，以显示对有害真菌和细菌的优异的抗生素效果，从而有效地用作食品，化妆品或药物的抗菌和抗真菌药物组合物，农药或防腐剂。 将具有抗生素活性的枯草芽孢杆菌S1沉积为沉积物。 KCTC 10888BP。 从枯草芽孢杆菌纯化肽的方法包括以下步骤：（a）培养枯草芽孢杆菌S1; （b）过滤由步骤（a）得到的培养液后，将滤液装入RP-HPLC（反相高效液相色谱法）中; （c）使用具有逐渐增加的乙腈浓度的洗脱液将蛋白质洗脱到步骤（b）的柱中; 和（d）从洗脱液中回收峰分数并研究其抗菌活性。 通过该方法纯化并显示抗菌和抗真菌活性的肽的特征在于其从枯草芽孢杆菌S1分泌，分子量为1.4-1.6KDa，并且对丝核菌（Rhizoctonia solani）和Colletotrichum coccodes显示出抗菌活性。 抗菌和抗真菌药物组合物，生物杀虫剂或食品，化妆品或药物的防腐剂包括该肽作为有效成分。 此外，乙腈的浓度从5％增加至65％。

公开(公告)号：KR102039400B1

公开(公告)日：2019-11-01

申请号：KR1020180098568

申请日：2018-08-23

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  박종관

IPC: C07K14/435 ,  A01N63/00 ,  A23K20/147 ,  A23K20/195 ,  A23L3/3463 ,  A23L33/18 ,  A61K8/64 ,  A61P31/04 ,  A61K38/00