Abstract:
PURPOSE: A microreactor and a microreactor system including the same are provided to prevent leakage of a leakage when the catalyst fixed in a carrier is filled therein and to increase a specific surface area of the catalyst. CONSTITUTION: A microreactor comprises a reaction solution inlet port, a microchannel, which is a region where an injected reactant reacts, and a reaction solution vent for ejecting the reaction solution that passed the microchannel. At least one microcolumn is formed in the microchannel. A height of the microcolumn is lower than a depth of the microchannel. A width of the microcolumn is the same with a width of the microchannel.
Abstract:
본 발명은 (A) 소정의 형상과 크기를 가진 마이크로몰드가 소정의 패턴으로 형성된 복제몰드에 실란화합물과, 산 또는 염기 및 알콜을 포함하는 실란혼합물을 충진하는 단계; (B) 상기 실란혼합물이 충진된 복제몰드에 C8~C16의 알칸을 가하는 단계; (C) 상기 (B) 단계에서 C8~C16의 알칸을 가한 복제몰드를 30~80℃로 가열하여 마이크로 입자를 경화하는 단계; 및 (D) 상기 경화된 마이크로 입자를 회수하고 세척하는 단계; 를 포함하여 제조되는 것을 특징으로 하는 면역분석용 마이크로 입자 및 이를 이용한 면역측정 방법에 관한 것이다.
Abstract:
PURPOSE: A droplet generating micro fluid chip, a droplet reacting micro fluid chip, and a multi-droplet reacting analyzer is provided to collect multiple generation unit elements and reacting unit elements, thereby examining and analyzing various reactions based on a micro droplet by one operation. CONSTITUTION: A droplet reacting micro fluid chip comprises a main channel(210), a reaction channel array(220), and an operation channel array(230). The reaction channel array is composed of a plurality of unit reaction channels branched from the main channel. The operation channel array is arranged in one side of the unit reaction channel, and a passage of the operation channel is separated from the unit reaction channel with a separation film. Flows of fluid inside the plurality of unit reaction channels are simultaneously controlled by the unit operation channels, thereby being integrated into one fluid flow. Three unit operation channels are arranged in each unit reaction channel. A first operation channel is utilized for a droplet trap valve, and second and third operation channels are utilized for a valve adding waves to droplets.
Abstract:
PURPOSE: A selective surface modification method of a microfluidic chip channel is provided to modify a surface without defects by simple and mild condition, and to selectively control the wettability of the microfluidic chip channel. CONSTITUTION: A selective surface modification method of a microfluidic chip channel comprises a first surface modification step filling trialkoxysilylalkyl methacrylate solution into the microfluidic chip channel; a step of filling the first surface modified microfluidic chip with solution with photoinitiator, and absorbing the photo initiator into pores of the microfluidic chip channel; a step of filling acrylic acid solution into a channel of a microfluidic chip irradiating ultraviolet ray to region for surface modification; and a step of filling the secondary surface treated microfluidic chip channel with acrylic acid solution comprising the photoinitiator, and thirdly surface treating by irradiating the region for surface modification with ultraviolet ray.
Abstract:
PURPOSE: A method for preparing DNA-conjugated hydrogel microparticles is provided to obtain the microparticles of uniform shape and size and have microparticles of various shapes. CONSTITUTION: A method for preparing DNA-conjugated hydrogel microparticles comprises: a step of preparing a replica mold formed in a predetermined pattern; a step of filling a composition containing acrylate group-modified probe DNA and photoinitiator in the replica mold; and a step of irradiating UV ray to the replica mold for photopolymerization. The replica mold is PDMS(polydimethylsiloxane) or polyisobutylene. The composition additionally contains polyethylene glycol or porogen.
Abstract:
본 발명은 선택적 단백질 고정화방법에 관한 것으로서, 보다 상세하게는 고분자 전해질을 이용한 다층박막 증착(Layer-by-layer deposition, LBL)의 표면 처리를 통하여 얻어진 표면 위에 정전기적 인력에 의한 선택적 단백질 고정화방법 및 상기 방법으로 항원 단백질을 고정화함으로써 항원-항체 반응을 유도할 수 있는 단백질 패터닝을 수행하는 방법에 관한 것이다. 본 발명의 단백질 고정화 방법은 단백질 활성 유지에 적합한 단백질의 3차원 입체구조가 유지되기에 유리한 조건이 되어 단백질 칩 내에서 탐침으로서의 효율을 높이고, 확산에 의한 단백질의 고정화가 아니므로 고분자 박막의 두께가 증가하여도 단백질 패턴의 크기가 동일하게 유지되고, 그 결과 동일 조건에서 형광세기가 항상 일정하므로 신호 세기에 대한 오차범위가 좁아져 보다 정확한 진단을 가능하게 한다. 따라서, 본 발명은 진단을 위한 단백질 칩의 제작에 사용될 수 있을 뿐만 아니라 미생물 칩, 세포 칩, 조직(tissue)칩을 제작하기 위한 미생물이나 세포의 고정화를 유도하는 단백질의 고정화시에 응용될 수 있다. 단백질 패터닝, 고분자 전해질, 다층박막 증착, 마이크로컨택트 프린팅,
Abstract:
PURPOSE: A manufacturing method for polymeric micro beads including micro-fluidic chip by increasing irradiation time of ultraviolet ray is provided to produce monomer in a micro-fluidic chip by extending irradiation time of ultraviolet ray on photocurable polymer droplets. CONSTITUTION: A manufacturing method for polymeric micro beads comprises a micro-fluidic chip(100) and a water tub(5). The water tub comprises a monomer inlet port(1b), a continuous phase inlet port(1a), a junction(2a) of monomer and continuous phase, a micro channel(2), and an outlet(2b). Photocurable polymer droplets are formed by passing the junction of monomer and continuous phase though the monomer. The polymeric micro beads are manufactured by curing the photocurable polymer droplets through an ultraviolet irradiation unit(6) on a real time basis.