公开(公告)号：KR1020140105980A

公开(公告)日：2014-09-03

申请号：KR1020130019851

申请日：2013-02-25

Applicant: 충남대학교산학협력단

Inventor： 이창수 ,  정헌호

IPC: C07K14/47 ,  G01N33/50 ,  G01N33/48 ,  G01N33/58

Abstract: The present invention relates to a method for simply analyzing a sugar chain on the surface of a cell in high sensitivity only by fixing, on a substrate without additionally labelling or transforming, a live cell or a tissue cell and, more specifically, to a method for analyzing a sugar chain on the surface of a cell which is characterized by comprising the steps of: (A) fixing a cell on the surface of a substrate; (B) treating lectin binding with biotin to the cell fixed in the (A) step; (C) treating a quantum dot binding with a living particle specifically binding with the biotin on the surface after treating lectin; and (D) detecting phosphor of the quantum dot.

Abstract translation: 本发明涉及一种仅通过固定在底物上而不附加标记或转化活细胞或组织细胞的方式简单地分析细胞表面上的糖链的方法，更具体地说，涉及一种方法 用于分析细胞表面上的糖链，其特征在于包括以下步骤：（A）将细胞固定在基底表面上; （B）将与生物素结合的凝集素处理至（A）步骤中固定的细胞; （C）处理凝集素后处理与表面特异性结合生物素的活性粒子结合的量子点; 和（D）检测量子点的荧光体。

公开(公告)号：KR1020140076212A

公开(公告)日：2014-06-20

申请号：KR1020120144523

申请日：2012-12-12

Applicant: 충남대학교산학협력단 ,  한국해양과학기술원

Inventor： 이창수 ,  정헌호 ,  장성찬 ,  이경은 ,  홍순규

IPC: G01N35/08 ,  C12M1/38 ,  C12Q1/02

Abstract: The present invention relates to a microfluidic device in which a concentration gradient and a temperature gradient are simultaneously created in a detection channel of a microchannel and, more specifically, to a microfluidic device comprising a microfluidic channel for a sample and a pair of microfluidic channels for tempreature adjustment. The microfluidic channel for a sample includes: two inlets; a gradient forming region for separating each fluid which is injected into the inlets, into multiple microchannels having a different concentration gradient; a detection channel for joining and moving the fluid separated into the multiple microchannels after passing the gradient forming region; and an outlet for discharging the fluid passed the detection channel. The pair of microfluidic channels for temperature adjustment, wherein each channel is formed in parallel on both sides of the detection channel of the microfluidic channel for a sample by being spaced out in a prefixed interval with the detection channel, and includes: an inlet; an outlet; and a microchannel through which a fluid injected into the inlet moved to the outlet, such that heating mediums having a different temperature are circulated.

Abstract translation: 本发明涉及一种微流体装置，其中在微通道的检测通道中同时产生浓度梯度和温度梯度，更具体地说，涉及包含用于样品的微流体通道和一对微流体通道的微流体装置， 温度调整 样品的微流体通道包括：两个入口; 用于将注入入口的每个流体分离成具有不同浓度梯度的多个微通道的梯度形成区域; 用于在通过所述梯度形成区域之后接合并移动分离成多个微通道的流体的检测通道; 以及用于排出流过检测通道的流体的出口。 一对用于温度调节的微流体通道，其中通过与检测通道间隔开的预定间隔，每个通道在用于样品的微流体通道的检测通道的两侧上平行地形成，并且包括：入口; 一个出口 以及将注入入口的流体移动到出口的微通道，使得具有不同温度的加热介质循环。

公开(公告)号：KR101393396B1

公开(公告)日：2014-05-12

申请号：KR1020130064711

申请日：2013-06-05

Applicant: 충남대학교산학협력단

Inventor： 이창수 ,  정헌호

IPC: G01N33/48 ,  C12Q1/02 ,  G01N35/08

Abstract: The present invention relates to a microfluidic device for analyzing the chemotaxis of bacteria and a chemotaxis analyzing method using the same and, more specifically, to a microfluidic device which is formed by attaching a mold (61) including a microfluidic channel to a substrate (51) by comprising: a first microfluidic channel (12) which is connected to a first discharge hole (13) and a first accommodating part (11) which accommodates first solution; a second microfluidic channel (22) which is connected to a second discharge hole (23) and a second accommodating part (21) which accommodates second solution; and a detection channel (31) in which the first microfluidic channel (12) and the second microfluidic channel (22) cross. Between the detection channel (31) and the second microfluidic channel (22), a pair of air channels (43) capable of accommodating air in the upper part is arranged across a crossing channel (42) of 1-10 μm in a width direction and a diffusion channel (41) is formed to have a depth of 1-10 μm which is shallower than 1/3 of the depth of the second microfluidic channel.

Abstract translation: 本发明涉及用于分析细菌趋化性的微流体装置和使用其的趋化性分析方法，更具体地说，涉及通过将包含微流体通道的模具（61）附着到基底（51）而形成的微流体装置 ）包括：第一微流体通道（12），其连接到第一排出孔（13）和容纳第一溶液的第一容纳部分（11）; 连接到第二排出孔（23）的第二微流体通道（22）和容纳第二溶液的第二容纳部分（21） 和第一微流体通道（12）和第二微流体通道（22）交叉的检测通道（31）。 在检测通道（31）和第二微流体通道（22）之间，在宽度方向上跨越1-10μm的交叉通道（42）布置能够容纳上部空气的一对空气通道（43） 并且扩散通道（41）形成为具有比第二微流体通道的深度的1/3浅的1-10μm的深度。

公开(公告)号：KR101480927B1

公开(公告)日：2015-01-13

申请号：KR1020130019851

申请日：2013-02-25

Applicant: 충남대학교산학협력단

Inventor： 이창수 ,  정헌호

IPC: C07K14/47 ,  G01N33/50 ,  G01N33/48 ,  G01N33/58

Abstract: 본 발명은 살아있는 세포 또는 조직세포를 별도로 표지하거나 변형하지 않고 기판에 고정시키는 것만으로 간편하고 높은 감도로 세포 표면의 당사슬을 분석할 수 있는 방법에 관한 것르로, 보다 상세하게는 (A) 기판의 표면에 세포를 고정화하는 단계; (B) (A)단계에서 고정화된 세포에 바이오틴이 결합된 렉틴을 처리하는 단계; (C) 렉틴의 처리 후 표면에 바이오틴과 특이결합하는 생체분자가 결합된 양자점을 처리하는 단계; 및 (D) 상기 양자점의 형광을 검출하는 단계; 를 포함하여 이루어지는 것을 특징으로 하는 세포 표면의 당사슬 분석 방법에 관한 것이다.

公开(公告)号：KR101421249B1

公开(公告)日：2014-07-18

申请号：KR1020120144523

申请日：2012-12-12

Applicant: 충남대학교산학협력단 ,  한국해양과학기술원

Inventor： 이창수 ,  정헌호 ,  장성찬 ,  이경은 ,  홍순규

IPC: G01N35/08 ,  C12M1/38 ,  C12Q1/02

Abstract: 본 발명은 미세유로의 검출채널에 농도구배 및 온도구배가 동시에 생성되는 미세유체장비에 관한 것으로, 보다 상세하게는 본 발명은 두 개의 주입구; 상기 주입구로 주입된 각 유체를 서로 다른 농도구배를 갖는 복수개의 미세채널로 분리하는 구배형성영역; 구배형성영역을 통과하여 복수개의 미세채널로 분리된 유체가 합류하여 이동되는 검출채널; 및 검출채널을 통과한 유체가 배출되는 배출구;를 포함하는 시료용 미세유로와 상기 시료용 미세유로의 검출채널의 양측면에 검출채널과 소정 간격을 두고 평행하게 각각 형성되며, 주입구; 배출구; 및 주입구로 주입된 유체가 배출구로 이동되는 미세유로로 구성되어 각각 다른 온도의 열매가 순환되는 한쌍의 온도조절용 미세유로;를 포함하여 구성되는 미세유체장비에 관한 것이다.

公开(公告)号：KR101244285B1

公开(公告)日：2013-03-18

申请号：KR1020110139102

申请日：2011-12-21

Applicant: 충남대학교산학협력단

Inventor： 이창수 ,  진시형 ,  정헌호

IPC: B81B1/00 ,  G01N33/48 ,  G01N35/08 ,  B81B7/00

Abstract: PURPOSE: A droplet generating micro fluid chip, a droplet reacting micro fluid chip, and a multi-droplet reacting analyzer is provided to collect multiple generation unit elements and reacting unit elements, thereby examining and analyzing various reactions based on a micro droplet by one operation. CONSTITUTION: A droplet reacting micro fluid chip comprises a main channel(210), a reaction channel array(220), and an operation channel array(230). The reaction channel array is composed of a plurality of unit reaction channels branched from the main channel. The operation channel array is arranged in one side of the unit reaction channel, and a passage of the operation channel is separated from the unit reaction channel with a separation film. Flows of fluid inside the plurality of unit reaction channels are simultaneously controlled by the unit operation channels, thereby being integrated into one fluid flow. Three unit operation channels are arranged in each unit reaction channel. A first operation channel is utilized for a droplet trap valve, and second and third operation channels are utilized for a valve adding waves to droplets.

Abstract translation: 目的：提供液滴生成微流体芯片，液滴反应微流体芯片和多液滴反应分析仪，以收集多代单元元素和反应单元元素，从而通过一次操作检测和分析基于微滴的各种反应 。 构成：液滴反应微流体芯片包括主通道（210），反应通道阵列（220）和操作通道阵列（230）。 反应通道阵列由从主通道分支的多个单元反应通道组成。 操作通道阵列布置在单元反应通道的一侧，并且通过分离膜将操作通道的通道与单元反应通道分离。 多个单元反应通道内的流体流由单元操作通道同时控制，从而被集成到一个流体流中。 在每个单元反应通道中安排三个单元操作通道。 第一操作通道用于液滴捕集阀，第二和第三操作通道用于向液滴中加入波的阀。