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公开(公告)号：KR101144199B1
公开(公告)日：2012-05-10
申请号：KR1020100012980
申请日：2010-02-11
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A biomarker for detecting persistent organic pollutants-specific exposure is provided to monitor and determine four kinds of pollutants. CONSTITUTION: A DNA microarray chip for detecting four kinds of persistent organic pollutants-specific exposure contains entire of nucleic acid sequence of Genebank NM_001040078(LGALS9C, similar to galectin 9 short isoform) and Genebank NM_016438(HIGD1B, HIG1 domain family, member 1B) genes, oligonucleotide, or complementary strand molecule thereof. The four kinds are chlordane, hexachlorobenzene, mirex, and toxaphene.
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公开(公告)号：KR101062784B1
公开(公告)日：2011-09-07
申请号：KR1020100012523
申请日：2010-02-10
Applicant: 한국과학기술연구원
Abstract: 본 발명은 잔류성 유기오염물질 중의 하나인 미렉스(mirex) 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 미렉스에 의해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 미렉스에 특이적 노출 여부를 확인하는 방법에 관한 것이며, 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 미렉스의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 미렉스에 의해 특이적으로 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
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公开(公告)号：KR1020110093129A
公开(公告)日：2011-08-18
申请号：KR1020100012980
申请日：2010-02-11
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6837 , C12Q1/6844 , C12Q1/6888 , C12Q2600/158 , G01N33/5067
Abstract: PURPOSE: A biomarker for detecting persistent organic pollutants-specific exposure is provided to monitor and determine four kinds of pollutants. CONSTITUTION: A DNA microarray chip for detecting four kinds of persistent organic pollutants-specific exposure contains entire of nucleic acid sequence of Genebank NM_001040078(LGALS9C, similar to galectin 9 short isoform) and Genebank NM_016438(HIGD1B, HIG1 domain family, member 1B) genes, oligonucleotide, or complementary strand molecule thereof. The four kinds are chlordane, hexachlorobenzene, mirex, and toxaphene.
Abstract translation: 目的：提供一种用于检测持久性有机污染物特异性暴露的生物标志物，以监测和确定四种污染物。 构成：用于检测四种持久性有机污染物特异性暴露的DNA微阵列芯片含有Genebank NM_001040078（LGALS9C，类似于galectin 9短异构体）和Genebank NM_016438（HIGD1B，HIG1结构域家族，成员1B）基因的全部核酸序列 ，寡核苷酸或其互补链分子。 四种是氯丹，六氯苯，灭蚁灵和毒杀芬。
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公开(公告)号：KR100985090B1
公开(公告)日：2010-10-04
申请号：KR1020080047946
申请日：2008-05-23
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q2600/142 , C12Q2600/158
Abstract: The present invention relates to a method for confirming the exposure on chrysene using a DNA fragment whose expression is increased or decreased specifically when it is exposed to chrysene. The method of the present invention is effective in determination of risk by chrysene and monitoring the chrysene exposure, so that it can be effectively used as a tool for examining the mechanism of chrysene induced toxicity.
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公开(公告)号：KR1020100018237A
公开(公告)日：2010-02-17
申请号：KR1020080076917
申请日：2008-08-06
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6876 , C12Q2600/158 , C40B40/06
Abstract: PURPOSE: A biomarker for confirming phenanthrene exposure is provided to monitor and determine phenanthrene pollution and to find a toxicity mechanism. CONSTITUTION: A biomarker for confirming phenanthrene contains a gene of which an expression is increased or decreased due to exposure to phenanthrene. A DNA microarray chip for confirming phenanthrene exposure contains oligotide having an entire or partial gene sequence or its complementary oligonuecleotide. A method for detecting the biomarker for confirming phenanthrene exposure comprises: a step of isolating RNA from a test person's somatic cells as an experimental group and normal somatic cells as a control group; a step of synthesizing cDNA of the experimental group and control group and labeling with different fluorescences; a step of hybridizing the labeled cDNA on a microarray chip; a step of analyzing DNA microarray chip; and a step of comparing expression level between control group and experimental group.
Abstract translation: 目的：提供一种用于确定菲暴露的生物标志物，用于监测和确定菲污染并找到毒性机制。 构成：用于确认菲的生物标志物含有由于暴露于菲而表达增加或减少的基因。 用于确认菲暴露的DNA微阵列芯片含有具有全部或部分基因序列的寡核苷酸或其互补寡核苷酸。 用于检测用于确认菲暴露的生物标志物的方法包括：将作为实验组和正常体细胞作为对照组的来自测试人体细胞的RNA分离的步骤; 合成实验组和对照组的cDNA并用不同荧光标记的步骤; 将标记的cDNA杂交到微阵列芯片上的步骤; 分析DNA微阵列芯片的步骤; 比较对照组和实验组之间的表达水平比较。
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Patent Agency Ranking
公开(公告)号：KR1020100013851A
公开(公告)日：2010-02-10
申请号：KR1020080075566
申请日：2008-08-01
Applicant: 한국과학기술연구원
CPC classification number: C40B40/06 , C12Q1/6837 , C12Q2600/142
Abstract: PURPOSE: A biomarker for detecting exposure to polycyclic aromatic hydrocarbons(PAHs) is provided to usefully monitor and determine contamination of PAHs and figure toxicity mechanism caused by the PAHs. CONSTITUTION: A biomarker for detecting exposure to polycyclic aromatic hydrocarbon(PAHs) contains a gene of which expression is decreased or increased by the exposure of the PAHs. A DNA microarray chip for detecting the exposure to the PAHs comprises oligonucleotide including entire or partial biomarker gene sequence and its complementary oligonucleotide. A method for detecting the exposure of the PAHs using the biomarker comprises: a step of isolating RNA from sample-derived human somatic cells as an experimental group and normal somatic cells as a control group; a step of synthesizing RNA to cDNA and labeling the experimental group and control group with different colors; a step of hybridizing the cDNA on the DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing the expression level between the experimental group and control group.
Abstract translation: 目的：提供一种检测多环芳烃（PAHs）暴露的生物标志物，有效监测和确定PAHs引起的多环芳烃污染物和毒性机制。 构成：用于检测多环芳烃（PAH）暴露的生物标志物含有通过多环芳烃的暴露降低或增加表达的基因。 用于检测对PAH的暴露的DNA微阵列芯片包括包含全部或部分生物标记基因序列及其互补寡核苷酸的寡核苷酸。 使用生物标志物检测多环芳烃暴露的方法包括：将来自样品衍生的人体细胞的RNA作为实验组和作为对照组的正常体细胞分离的步骤; 将RNA合成cDNA的步骤，并对不同颜色的实验组和对照组进行标记; 在DNA微阵列芯片上杂交cDNA的步骤; 分析DNA微阵列芯片的步骤; 并比较实验组与对照组之间的表达水平。
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公开(公告)号：KR1020090121842A
公开(公告)日：2009-11-26
申请号：KR1020080047946
申请日：2008-05-23
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q2600/142 , C12Q2600/158 , C40B40/06
Abstract: PURPOSE: A biomarker for confirming exposure to chrysene is provided to monitor and determine chrysene contamination and study of toxic mechanism by the chrysene. CONSTITUTION: A method for detecting biomarker for confirming exposure of chrysene comprises: a step of isolating RNA from human somatic cell of sample as experimental group and somatic cell of control group; a step of synthesizing cDNA then hybridizing the experimental group and control group with different fluorescences; a step of hybridizing the cDNA on a DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing the expression level of biomarker between control group and experimental group.
Abstract translation: 目的：确定暴露于中国的生物标志物，用于监测和确定中华人民共和国污染物，并研究其毒性机制。 构成：检测生物标志物用于确认暴露的生物标志物的方法包括：将样品的人体细胞的RNA分离为对照组的实验组和体细胞的步骤; 合成cDNA的步骤，然后用实验组和对照组与不同的荧光素杂交; 将cDNA杂交到DNA微阵列芯片上的步骤; 分析DNA微阵列芯片的步骤; 比较对照组和实验组生物标志物的表达水平。
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公开(公告)号：KR101758701B1
公开(公告)日：2017-07-17
申请号：KR1020160029879
申请日：2016-03-11
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: 본발명은마이크로 RNA를이용한톨루엔노출여부확인용바이오마커및 이를이용한확인방법에관한것으로, 보다상세하게는톨루엔노출에의해엑소좀유래마이크로 RNA와세포주유래마이크로 RNA에서공통적으로발현수준이 1.5배이상과발현되는마이크로 RNA 81종과 0.66배이하저발현되는마이크로 RNA 6종을선별함으로써, 총 87종의마이크로 RNA를바이오마커로이용하여톨루엔의모니터링및 위해성을판정하는데유용하게사용할수 있으며, 톨루엔에의해야기되는독성작용기작을규명하는도구로이용할수 있음을알 수있다.
Abstract translation: 本发明涉及一种验证方法，使用生物标记物和该确认是否甲苯曝光使用微RNA，更具体地涉及表达的外定位衍生的微RNA的公共电平，并通过甲苯曝光从微RNA衍生的细胞系是至少1.5倍 通过选择81种过表达微小RNA和小于0.66倍的微RNA 6种低表达的，并且可以是确定由在生物标志物共87种使用微RNA监测和甲苯的风险是有用的，由甲苯 它可以被用作识别造成的毒性作用机制的工具。
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公开(公告)号：KR101735417B1
公开(公告)日：2017-05-15
申请号：KR1020140168698
申请日：2014-11-28
Applicant: 한국과학기술연구원
Abstract: 본발명은환경중에서노출되는휘발성유기화합물중의하나인헥산알(Hexanal)의노출여부확인용바이오마커및 이를이용한확인방법에관한것으로, 구체적으로헥산알에의해발현변화를일으키는특이적인마이크로 RNA 및이를이용하여헥산알에대한노출여부를확인하는방법에관한것이다. 본발명의바이오마커는마이크로 RNA 마이크로어레이칩을통하여선별된반응마이크로 RNA이며, 이를이용하여헥산알의오염을모니터링및 판정하는데유용하게사용될수 있으며, 헥산알에의해유발되는독성작용기작을규명하는도구로이용될수 있다.
Abstract translation: 本发明涉及的己醛曝光的一个检查是否生物标志物和使用其的被暴露在环境中，特定微小RNA和特异性是由己醛引起的表达的变化的挥发性有机化合物的（己醛）验证方法 该方法包括以下步骤： 本发明的生物标志物通过微RNA微阵列所选择的反应微RNA，使用它们可以在监测有用和确定的己醛的污染，工具来识别其通过己醛引起的毒性更小的官能团 它可以作为一个。
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60.发明公开디젤 배기 미립자(diesel exhaust particle) 노출에 따른 염증반응 관련 유전자 발현 여부 확인용 바이오마커 및 이를 이용한 확인 방법 有权用于鉴定与暴露于柴油机废气颗粒后的炎症反应有关的基因的柴油废气颗粒生物标志物和使用相同的鉴定方法
公开(公告)号：KR1020170022361A
公开(公告)日：2017-03-02
申请号：KR1020150117209
申请日：2015-08-20
Applicant: 한국과학기술연구원
Abstract: 본발명은환경중 노출되는미세입자중의하나인디젤배기미립자(diesel exhaust particle) 노출여부확인용염증반응관련바이오마커및 이를이용한확인방법에관한것으로, 구체적으로디젤배기미립자의노출에의해유전자발현이증가또는감소하는염증반응관련바이오마커및 이를이용한디젤배기미립자에특이적노출여부를확인하는방법에관한것이며, 본발명의바이오마커는 DNA 마이크로어레이칩을통하여선별된염증반응관련유전자들을바이오마커로이용하여환경시료에서디젤배기미립자의오염을모니터링및 판정하는데유용하게사용될수 있으며, 디젤배기미립자에의해특이적으로유발되는독성작용기작을규명하는도구로이용될수 있다.
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