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公开(公告)号：US20220187191A1
公开(公告)日：2022-06-16
申请号：US17686229
申请日：2022-03-03
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique
Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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公开(公告)号：US20220152606A1
公开(公告)日：2022-05-19
申请号：US17590543
申请日：2022-02-01
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Swati Ranade , Vishal Sharma , Austin Payne , Sam Tuck
Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).
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公开(公告)号：US11273439B2
公开(公告)日：2022-03-15
申请号：US16906337
申请日：2020-06-19
Applicant: Bio-Rad Laboratories, Inc
Inventor： Kalyan Handique , Swati Ranade , Vishal Sharma , Austin Payne , Sam Tuck
Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).
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公开(公告)号：US20210283609A1
公开(公告)日：2021-09-16
申请号：US17333337
申请日：2021-05-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Priyadarshini Gogoi , Saedeh Javdani Sepehri , Kyle Gleason
IPC: B01L3/00 , G01N15/10 , G01N15/14 , C12M1/00 , C12Q1/6834 , C12Q1/6841 , G01N33/574
Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
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公开(公告)号：US10975422B2
公开(公告)日：2021-04-13
申请号：US16049057
申请日：2018-07-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Priyadarshini Gogoi , Saedeh Sepehri Javdani , Yi Zhou
IPC: C12Q1/68 , G01N33/487 , G01N33/574 , C12Q1/6841 , C12Q1/6844 , C12Q1/686 , G01N27/447
Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.
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Patent Agency Ranking
公开(公告)号：US20250020574A1
公开(公告)日：2025-01-16
申请号：US18887450
申请日：2024-09-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Austin Payne , Vishal Sharma , Kyle Gleason , Priyadarshini Gogoi , Karthik Ganesan , Brian Boniface , Will Chow
IPC: G01N15/14 , B01L3/00 , B01L3/02 , C12M1/00 , G01N1/20 , G01N1/28 , G01N1/40 , G01N15/01 , G01N15/10 , G01N15/1433 , G01N15/149 , G01N35/00 , G01N35/10 , G06V10/141 , G06V10/145 , G06V10/147 , G06V20/69
Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
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公开(公告)号：US12162015B2
公开(公告)日：2024-12-10
申请号：US17499454
申请日：2021-10-12
Applicant: Bio-Rad Europe GmbH , Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Ronald Lebofsky , Emilie Celine Claudie Macke , Rebecca Dievart , Christophe Quiring , Sophie Pierre
IPC: B01L3/00
Abstract: A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.
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公开(公告)号：US20240307880A1
公开(公告)日：2024-09-19
申请号：US18674938
申请日：2024-05-27
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , Kyle Gleason , Austin Payne , Priyadarshini Gogoi , Christopher Siemer , Yi Zhou , Saedeh Javdani Sepehri
CPC classification number: B01L3/502761 , B01L3/502715 , B01L3/527 , B01L3/5635 , B01L7/00 , G01N35/0098 , B01L2200/027 , B01L2200/0647 , B01L2200/0652 , B01L2200/0684 , B01L2300/06 , B01L2300/0816 , B01L2300/0832 , B01L2300/0864 , B01L2300/1822 , B01L2300/1827 , B01L2300/185 , B01L2400/0487 , B01L2400/0683
Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.
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公开(公告)号：US20240060872A1
公开(公告)日：2024-02-22
申请号：US18384323
申请日：2023-10-26
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique
CPC classification number: G01N15/1484 , C12M47/04 , B01L3/502715 , B01L3/502761 , B01L3/021 , G01N1/28 , G01N1/405 , B01L3/502746 , G01N1/20 , G01N1/40 , B01L2300/0636 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0877 , G01N1/4077 , G01N2015/149 , G01N2035/00158
Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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公开(公告)号：US11814671B2
公开(公告)日：2023-11-14
申请号：US17170576
申请日：2021-02-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kalyan Handique , John Stark , Yadwinder Deol , Vishal Sharma
IPC: C12Q1/6811 , C12Q1/6874 , C12N15/10
CPC classification number: C12Q1/6811 , C12N15/1065 , C12Q1/6874 , C12Q2563/179 , C12Q2565/629 , C12Q2600/178
Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.
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