公开(公告)号：US10889863B2

公开(公告)日：2021-01-12

申请号：US16102583

申请日：2018-08-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/68 ,  C12Q1/70 ,  C12Q1/6883 ,  C12Q1/6851

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US10603650B2

公开(公告)日：2020-03-31

申请号：US16222865

申请日：2018-12-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Sameer Walavalkar ,  Axel Scherer

IPC: B01J19/00 ,  G01N21/65 ,  C12Q1/689

Abstract: Methods and systems for nanopillar sensors are described. Nanopillars can be defined on a substrate, and metal deposited on the nanopillars. A thermal treatment can reflow the metal on the nanopillars forming metallic bulbs on the top end of the nanopillars. These structures can have enhanced optical detection when functionalized with biological agents, or can detect gases, particles and liquids through interaction with the metal layer on the nanopillars.

公开(公告)号：US10509018B2

公开(公告)日：2019-12-17

申请号：US14928631

申请日：2015-10-30

Applicant: California Institute of Technology

Inventor： Stephen R. Quake ,  Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B01D57/02 ,  B01D61/18 ,  B01D61/28 ,  B01D63/08 ,  B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01F13/10 ,  B01L3/00 ,  F04B43/04 ,  F16K99/00 ,  G01N27/447 ,  G01N33/50 ,  C12M3/06 ,  C12M1/34 ,  G01N33/569 ,  B01F7/00

Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

公开(公告)号：US10366856B2

公开(公告)日：2019-07-30

申请号：US15442408

申请日：2017-02-24

Applicant: California Institute of Technology

Inventor： Axel Scherer ,  William M. Jones ,  Danil M. Lukin ,  Sameer Walavalkar ,  Chieh-feng Chang

IPC: H01J19/24 ,  H01J9/02 ,  H01J21/02

Abstract: Nanoscale field-emission devices are presented, wherein the devices include at least a pair of electrodes separated by a gap through which field emission of electrons from one electrode to the other occurs. The gap is dimensioned such that only a low voltage is required to induce field emission. As a result, the emitted electrons energy that is below the ionization potential of the gas or gasses that reside within the gap. In some embodiments, the gap is small enough that the distance between the electrodes is shorter than the mean-free path of electrons in air at atmospheric pressure. As a result, the field-emission devices do not require a vacuum environment for operation.

公开(公告)号：US20190117071A1

公开(公告)日：2019-04-25

申请号：US16204942

申请日：2018-11-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Axel Scherer ,  Muhammad Mujeeb-U-Rahman ,  Meisam Honavar Nazari ,  Muhammad Musab Jilani

IPC: A61B5/00 ,  A61B5/145 ,  G16H40/67

Abstract: A sensing device allows detection of biological quantities in ways that are minimally invasive. Micrometer or nanometer sized needles allow sensing of bodily fluids in a minimally invasive method. The device comprises electronics and power harvesting. Antennas or coils allow communication and power harvesting from an external device, which can be attached to smartphones to allow operation of a camera and camera light for biosensing.

公开(公告)号：US10006855B2

公开(公告)日：2018-06-26

申请号：US15359296

申请日：2016-11-22

Applicant: California Institute of Technology

Inventor： Axel Scherer ,  Frank T. Hartley

IPC: G01N21/35 ,  G01N21/3577 ,  G01J3/42 ,  G01J3/02 ,  G01J3/18 ,  G01J3/36 ,  G01J3/12

CPC classification number: G01N21/35 ,  G01J3/0208 ,  G01J3/0256 ,  G01J3/108 ,  G01J3/18 ,  G01J3/36 ,  G01J3/42 ,  G01J2003/1213 ,  G01N21/3563 ,  G01N21/3577 ,  G01N2021/3148 ,  G01N2201/0245 ,  G01N2201/0638

Abstract: MIR spectroscopy systems comprising hierarchical spectral dispersion that enables fine spectral resolution and high sensitivity spectroscopy are disclosed. Hierarchical spectral dispersion is derived by employing at least two diffractive lens arrays, located on either side of a test sample, each receiving input radiation having an input spectral range and distributing the input radiation into a plurality of output signals, each having a fraction of the spectral range of the input radiation. As a result, the signal multiplication factor of the two arrays is multiplied in a manner that mitigates the propagation of wavelength harmonics through the system. In some embodiments, an emitter array comprising a plurality of spectrally selective emitters provides the input MIR radiation to a spectroscopy system. In some embodiments, spectrally selective detectors are used to detect narrow spectral components in the radiation after they have passed through the test sample.

公开(公告)号：US09960238B2

公开(公告)日：2018-05-01

申请号：US14720429

申请日：2015-05-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  SANOFI

Inventor： Aditya Rajagopal ,  Chieh-feng Chang ,  Oliver Plettenburg ,  Stefan Petry ,  Axel Scherer ,  Charles L. Tschirhart

IPC: H01L29/00 ,  H01L29/41 ,  H01L29/73 ,  H01L29/78 ,  B82Y15/00 ,  H01L29/732 ,  H01L29/06 ,  G01N27/414 ,  H01L29/45 ,  H01L29/49 ,  H01L29/735

CPC classification number: H01L29/413 ,  B82Y15/00 ,  G01N27/4145 ,  G01N27/4146 ,  H01L29/0676 ,  H01L29/45 ,  H01L29/49 ,  H01L29/73 ,  H01L29/732 ,  H01L29/735 ,  H01L29/78

Abstract: Systems and methods for molecular sensing are described. Molecular sensors are described which are based on field-effect or bipolar junction transistors. These transistors have a nanopillar with a functionalized layer contacted to either the base or the gate electrode. The functional layer can bind molecules, which causes an electrical signal in the sensor.

公开(公告)号：US20170122939A1

公开(公告)日：2017-05-04

申请号：US15232651

申请日：2016-08-09

Applicant: California Institute of Technology

Inventor： Sameer Sudhir Walavalkar ,  Andrew Peter Homyk ,  William Maxwell Jones ,  Axel Scherer ,  Scott Fraser ,  Thai Viet Truong

IPC: G01N33/543 ,  C23C14/00 ,  C23C14/34 ,  C23C14/18 ,  C23C14/02 ,  C23F4/00

CPC classification number: C23C14/0021 ,  C23C14/021 ,  C23C14/025 ,  C23C14/185 ,  C23C14/3464 ,  C23F4/00 ,  G01N21/554 ,  G01N21/6458 ,  G01N21/648 ,  G01N21/78

Abstract: Devices, systems, and methods for detection of an analyte in a sample are disclosed. In some embodiments, an optical sensor can include a metallic layer and a plurality of dielectric pillars extending through the metallic layer. A plurality of regions of concentrated light can be supported in proximity to the ends of the plurality of dielectric pillars when a surface of the metallic layer is illuminated. Concentrated light within one or more of these regions can interact with an analyte molecule, allowing for detection of the analyte.

公开(公告)号：US09618477B2

公开(公告)日：2017-04-11

申请号：US13967108

申请日：2013-08-14

Applicant: California Institute of Technology

Inventor： Sameer Walavalkar ,  Axel Scherer ,  Thomas A. Tombrello ,  Aditya Rajagopal ,  Andrew P. Homyk ,  Erika Garcia

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487 ,  B82Y30/00

CPC classification number: G01N27/447 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Methods and devices for sequencing nucleic acids are disclosed herein. Devices are also provided herein for measuring DNA with nano-pores sized to allow DNA to pass through the nano-pore. The capacitance can be measured for the DNA molecule passing through the nano-pore. The capacitance measurements can be correlated to determine the sequence of base pairs passing through the nano-pore to sequence the DNA.

公开(公告)号：US09606060B2

公开(公告)日：2017-03-28

申请号：US13740112

申请日：2013-01-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Samson Chen ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6408

Abstract: A device and method are described in which the lifetime of a fluorescent species or fluorophores is detected in the absence of any optical filter. Based on the measured fluorescent lifetimes, molecules or compounds attached to a fluorophores such as small organic molecules, polymers, peptides, saccharides and nucleic acids can be identified or assayed.