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    MICROFLUIDIC NUCLEIC ACID ANALYSIS
    5.
    发明申请
    MICROFLUIDIC NUCLEIC ACID ANALYSIS 审中-公开

    公开(公告)号：US20200147608A1

    公开(公告)日：2020-05-14

    申请号：US16396137

    申请日：2019-04-26

    Applicant: California Institute of Technology

    Inventor： Jong Wook Hong ,  Vincent Studer ,  W. French Anderson ,  Stephen R. Quake ,  Jared Leadbetter

    IPC: B01L3/00 ,  C12Q1/6806

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. Individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. Cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    Microfluidic nucleic acid analysis
    7.
    发明授权
    Microfluidic nucleic acid analysis 有权
    微流控核酸分析

    公开(公告)号：US09579650B2

    公开(公告)日：2017-02-28

    申请号：US14494284

    申请日：2014-09-23

    Applicant: California Institute of Technology

    Inventor： Jong Wook Hong ,  Vincent Studer ,  W. French Anderson ,  Stephen R. Quake ,  Jared Leadbetter

    IPC: C12Q1/68 ,  B01L3/00

    CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    Abstract translation: 来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。 根据本发明的一个实施方案，可通过稀释，分选和/或分割在微流体室中分离单个或小组的细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化得到的核酸。 随后洗脱纯化的核酸之后可以连接和细胞转化，全部在相同的微流体芯片内。 在一个具体应用中，细胞分离，裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

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