公开(公告)号：US10509018B2

公开(公告)日：2019-12-17

申请号：US14928631

申请日：2015-10-30

Applicant: California Institute of Technology

Inventor： Stephen R. Quake ,  Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B01D57/02 ,  B01D61/18 ,  B01D61/28 ,  B01D63/08 ,  B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01F13/10 ,  B01L3/00 ,  F04B43/04 ,  F16K99/00 ,  G01N27/447 ,  G01N33/50 ,  C12M3/06 ,  C12M1/34 ,  G01N33/569 ,  B01F7/00

Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

公开(公告)号：US20160123958A1

公开(公告)日：2016-05-05

申请号：US14928631

申请日：2015-10-30

Applicant: California Institute of Technology

Inventor： Stephen R. Quake ,  Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer

IPC: G01N33/50 ,  B01L3/00

Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

Abstract translation: 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。

公开(公告)号：US09176137B2

公开(公告)日：2015-11-03

申请号：US14216740

申请日：2014-03-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Stephen R. Quake ,  Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/569 ,  B01D57/02 ,  B01D61/18 ,  B01D61/28 ,  B01D63/08 ,  B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01F13/10 ,  F04B43/04 ,  F16K99/00 ,  G01N27/447 ,  G01N33/50 ,  C12M3/06 ,  C12M1/34 ,  B01F7/00 ,  B01L3/00

CPC classification number: G01N33/00 ,  B01D57/02 ,  B01D61/18 ,  B01D61/28 ,  B01D63/081 ,  B01D63/088 ,  B01D2313/08 ,  B01D2313/90 ,  B01F5/0646 ,  B01F5/0647 ,  B01F7/00 ,  B01F11/0071 ,  B01F13/0059 ,  B01F13/1013 ,  B01F13/1022 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/082 ,  C12M23/16 ,  C12M41/46 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N15/06 ,  G01N27/44743 ,  G01N27/44791 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/56966 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.

Abstract translation: 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。

公开(公告)号：US20150183633A1

公开(公告)日：2015-07-02

申请号：US14503252

申请日：2014-09-30

Applicant: California Institute of Technology

Inventor： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

IPC: B81B3/00

CPC classification number: B32B3/00 ,  B01J2219/00355 ,  B01J2219/00378 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00439 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0688 ,  B32B2037/1081 ,  B81B3/0032 ,  B81B3/0051 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  C12Q1/6874 ,  F04B43/043 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/2224 ,  Y10T137/87716 ,  Y10T137/87804 ,  Y10T137/87812 ,  Y10T137/87893 ,  Y10T156/10 ,  Y10T156/1002 ,  Y10T156/1064 ,  Y10T428/24479 ,  Y10T428/2457 ,  Y10T428/24612 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

公开(公告)号：US20140322489A1

公开(公告)日：2014-10-30

申请号：US14218872

申请日：2014-03-18

Applicant: California Institute of Technology

Inventor： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

IPC: F16K13/00

CPC classification number: B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。